TRAF6_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRAF6_RAT
• UniProt AC: B5DF45
• Protein Name: TNF receptor-associated factor 6
• Gene Name: Traf6
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 530
• Subcellular Localization: Cytoplasm . Cytoplasm, cell cortex . Nucleus . Lipid droplet . RSAD2/viperin recruits it to the lipid droplet.
• Protein Description: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Mediates activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production..
• Protein Sequence: MSLLNCENSCASSQSSSDCCAAMANSCSAAMKDDSVSGCVSTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACITKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCVQKMELRHLEDHQVHCEFALVICPQCQRFFQKCQINKHIIEDCPRRQVSCVNCAVPMPYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPVPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLSLRPCDASSPSRGCRPEDPNYEETVKQLEGRLVRQDHQIRELTAKMETQSMHVSELKRTIRSLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAVIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
283	Phosphorylation	AVHNVNLSLRPCDAS HHHHCCCCCCCCCCC	19.68	27097102
290	Phosphorylation	SLRPCDASSPSRGCR CCCCCCCCCCCCCCC	29.54	28432305
291	Phosphorylation	LRPCDASSPSRGCRP CCCCCCCCCCCCCCC	28.72	28432305
293	Phosphorylation	PCDASSPSRGCRPED CCCCCCCCCCCCCCC	43.16	28432305

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Traf6	B5DF45	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
63	K	ubiquitylation	LRRC19 induces 'Lys-63' ubiquitination (By similarity).	-
63	K	ubiquitylation	This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules.	-
124	K	Sumoylation	Sumoylated on Lys-124, Lys-142 and Lys-461 with SUMO1.	-
124	K	ubiquitylation	Polyubiquitinated on Lys-124 by TRAF3IP2; after cell stimulation with IL17A (By similarity).	-
142	K	Sumoylation	Sumoylated on Lys-124, Lys-142 and Lys-461 with SUMO1.	-
461	K	Sumoylation	Sumoylated on Lys-124, Lys-142 and Lys-461 with SUMO1.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRAF6_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NUMBL_RAT	Numbl	physical	20079715
NRIF1_RAT	Zfp110	physical	16252010
SQSTM_RAT	Sqstm1	physical	16252010
IKKB_RAT	Ikbkb	physical	16079148
KPCG_RAT	Prkcg	physical	16079148
KHDR1_RAT	Khdrbs1	physical	16079148
TAGL_RAT	Tagln	physical	26291555

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.