KHDR1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KHDR1_RAT
• UniProt AC: Q91V33
• Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• Gene Name: Khdrbs1 {ECO:0000312|RGD:621459}
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 443
• Subcellular Localization: Nucleus . Cytoplasm . Membrane . Predominantly located in the nucleus but also located partially in the cytoplasm.
• Protein Description: Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. May not be involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species. RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner. In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1. Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (By similarity)..
• Protein Sequence: MQRRDDPAARLTRSSGRSCSKDPSGAHPSVRLTPSRPSPLPHRSRGGGGGPRGGARASPATQPPPLLPPSNPGPDATVVGSAPTPLLPPSATAAAKMEPENKYLPELMAEKDSLDPSFTHAMQLLSVEIEKIQKGESKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPSRGRGVSVRGRGAAPPPPPVPRGRGVGPPRGALVRGTPVRGSITRGATVTRGVPPPPTVRGAPTPRARTAGIQRIPLPPTPAPETYEDYGYDDSYAEQSYEGYEGYYSQSQGESEYYDYGHGELQDSYEAYGQDDWNGTRPSLKAPPARPVKGAYREHPYGRY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Phosphorylation	DDPAARLTRSSGRSC CCHHHHHHHCCCCCC	23.97	18779572
14	Phosphorylation	PAARLTRSSGRSCSK HHHHHHHCCCCCCCC	31.73	27097102
15	Phosphorylation	AARLTRSSGRSCSKD HHHHHHCCCCCCCCC	34.63	27097102
18	Phosphorylation	LTRSSGRSCSKDPSG HHHCCCCCCCCCCCC	26.69	28432305
20	Phosphorylation	RSSGRSCSKDPSGAH HCCCCCCCCCCCCCC	41.14	27097102
21	Acetylation	SSGRSCSKDPSGAHP CCCCCCCCCCCCCCC	77.36	-
24	Phosphorylation	RSCSKDPSGAHPSVR CCCCCCCCCCCCCCC	59.76	27097102
29	Phosphorylation	DPSGAHPSVRLTPSR CCCCCCCCCCCCCCC	15.25	25403869
33	Phosphorylation	AHPSVRLTPSRPSPL CCCCCCCCCCCCCCC	14.51	27097102
35	Phosphorylation	PSVRLTPSRPSPLPH CCCCCCCCCCCCCCC	52.14	27097102
38	Phosphorylation	RLTPSRPSPLPHRSR CCCCCCCCCCCCCCC	37.65	27097102
45	Asymmetric dimethylarginine	SPLPHRSRGGGGGPR CCCCCCCCCCCCCCC	47.97	-
45	Methylation	SPLPHRSRGGGGGPR CCCCCCCCCCCCCCC	47.97	-
52	Asymmetric dimethylarginine	RGGGGGPRGGARASP CCCCCCCCCCCCCCC	60.49	-
52	Methylation	RGGGGGPRGGARASP CCCCCCCCCCCCCCC	60.49	-
58	Phosphorylation	PRGGARASPATQPPP CCCCCCCCCCCCCCC	14.98	29779826
61	Phosphorylation	GARASPATQPPPLLP CCCCCCCCCCCCCCC	45.02	27097102
70	Phosphorylation	PPPLLPPSNPGPDAT CCCCCCCCCCCCCCE	54.43	27097102
77	Phosphorylation	SNPGPDATVVGSAPT CCCCCCCEEECCCCC	24.09	27097102
81	Phosphorylation	PDATVVGSAPTPLLP CCCEEECCCCCCCCC	21.33	25575281
84	Phosphorylation	TVVGSAPTPLLPPSA EEECCCCCCCCCCCC	26.70	-
96	Acetylation	PSATAAAKMEPENKY CCCCHHHHCCCCHHC	38.78	22902405
113	Phosphorylation	ELMAEKDSLDPSFTH HHHHCCCCCCCHHHH	45.82	-
137	Phosphorylation	EKIQKGESKKDDEEN HHHHCCCCCCCCCCC	54.39	28432305
150	Phosphorylation	ENYLDLFSHKNMKLK CCHHHHHHHCCCCCH	40.63	23984901
165	Acetylation	ERVLIPVKQYPKFNF HEEEEEHHHCCCCCE	38.47	13582855
169	Acetylation	IPVKQYPKFNFVGKI EEHHHCCCCCEEHHH	48.26	22902405
175	Acetylation	PKFNFVGKILGPQGN CCCCEEHHHHCCCCH	29.47	22902405
183	Phosphorylation	ILGPQGNTIKRLQEE HHCCCCHHHHHHHHH	34.40	23984901
202	Phosphorylation	ISVLGKGSMRDKAKE EEEECCCHHHHHHHH	17.71	23984901
282	Methylation	NGVPEPSRGRGVSVR CCCCCCCCCCCCCCC	49.93	-
284	Methylation	VPEPSRGRGVSVRGR CCCCCCCCCCCCCCC	41.03	-
291	Methylation	RGVSVRGRGAAPPPP CCCCCCCCCCCCCCC	22.48	-
304	Asymmetric dimethylarginine	PPPVPRGRGVGPPRG CCCCCCCCCCCCCCC	37.59	-
304	Methylation	PPPVPRGRGVGPPRG CCCCCCCCCCCCCCC	37.59	-
310	Methylation	GRGVGPPRGALVRGT CCCCCCCCCCEECCC	46.26	-
315	Methylation	PPRGALVRGTPVRGS CCCCCEECCCCCCCC	44.08	-
320	Methylation	LVRGTPVRGSITRGA EECCCCCCCCEECCC	34.05	-
325	Methylation	PVRGSITRGATVTRG CCCCCEECCCEEECC	30.95	13582883
331	Asymmetric dimethylarginine	TRGATVTRGVPPPPT ECCCEEECCCCCCCC	39.51	-
331	Methylation	TRGATVTRGVPPPPT ECCCEEECCCCCCCC	39.51	26494635
340	Methylation	VPPPPTVRGAPTPRA CCCCCCCCCCCCCCC	37.73	-
346	Methylation	VRGAPTPRARTAGIQ CCCCCCCCCCCCCCC	38.90	-
387	Phosphorylation	YEGYEGYYSQSQGES CCCCCCCCCCCCCCC	15.87	-
390	Phosphorylation	YEGYYSQSQGESEYY CCCCCCCCCCCCEEE	33.92	-
435	Phosphorylation	ARPVKGAYREHPYGR CCCCCCCCCCCCCCC	25.79	-
440	Phosphorylation	GAYREHPYGRY---- CCCCCCCCCCC----	19.01	-
443	Phosphorylation	REHPYGRY------- CCCCCCCC-------	20.67	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
84	T	Phosphorylation	Kinase	MAPK1	P63086	Uniprot
435	Y	Phosphorylation	Kinase	PTK6	-	Uniprot
440	Y	Phosphorylation	Kinase	PTK6	-	Uniprot
443	Y	Phosphorylation	Kinase	PTK6	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KHDR1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KHDR1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KCAB2_RAT	Kcnab2	physical	15062979

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.