dbPTM

REF2P_DROME - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	REF2P_DROME
UniProt AC	P14199
Protein Name	Protein ref(2)P
Gene Name	ref(2)P
Organism	Drosophila melanogaster (Fruit fly).
Sequence Length	599
Subcellular Localization	Nucleus . Cytoplasm .
Protein Description	Required for selective autophagy activation by ubiquitinated proteins. [PubMed: 22622177 Implicated in sigma rhabdovirus multiplication and necessary for male fertility. Involved in activating transcription of Drs]
Protein Sequence	MPEKLLKITYQGAGPQKKINAYLRMPSQNYTILRREIELYLFQERQLPKCDVRTFWIDADKDEIEIVNQNDYEIFLAKCESNMHVQVAPLAPVEEPKATKQEGSSANAEAPSVDDPSNFTIHDAVECDGCGLAPLIGFRYKCVQCSNYDLCQKCELAHKHPEHLMLRMPTNNGPGMVDAWFTGPGLGRRSGRRSRGHCPFQETNQADPAGEPARDSRRERRQARRHAGVLTQFVEMMTNLPLNTTTATAPAEPQKPKAAEQTESPPQAEPTVTAEKAAESEAKPTEPKKVNTDQSVPRTEDPVTTPRSTQPTTPVINLDNISQIVPPEYMSAGIEILNNFSEMFSKIIDTTEGGDSGIFAPSTTPSAENKKPEEQGQSSGQSGASSANQSAVPSAAPSANQSNVPSANQSATPSISGSIPDAQLETEPLNPKPSETTTETEQERRRSDSLDPEWQLIDNAYSANNSNLINLDTTNPTAAPQEPVRDFGQLGELLRQHMNEEARVEQASANTQTAQVDTVSTSTSTTSVTTNSVGTSPAAPDDKRTVPVYHTDESINKSIHAMMAMGFSNEGAWLTQLLESVQGNISAALDVMNVSQNRN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
146	Phosphorylation	RYKCVQCSNYDLCQK EEEEEECCCHHHHHH	22.72	25749252
194	Phosphorylation	GRRSGRRSRGHCPFQ CCCCCCCCCCCCCCC	41.05	19429919
262	Phosphorylation	KPKAAEQTESPPQAE CCCHHHCCCCCCCCC	29.90	23607784
264	Phosphorylation	KAAEQTESPPQAEPT CHHHCCCCCCCCCCC	45.31	23607784
305	Phosphorylation	RTEDPVTTPRSTQPT CCCCCCCCCCCCCCC	19.55	22817900
350	Phosphorylation	MFSKIIDTTEGGDSG HHHHHHHCCCCCCCC	19.15	19429919
351	Phosphorylation	FSKIIDTTEGGDSGI HHHHHHCCCCCCCCC	28.96	19429919
356	Phosphorylation	DTTEGGDSGIFAPST HCCCCCCCCCCCCCC	36.74	19429919
447	Phosphorylation	TEQERRRSDSLDPEW HHHHHHHHCCCCHHH	29.73	22817900
554	Phosphorylation	PVYHTDESINKSIHA CCEECCHHHHHHHHH	33.98	27794539

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of REF2P_DROME !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of REF2P_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of REF2P_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ACT5_DROME	Act87E	physical	14605208
Y678_DROME	CG2678	physical	14605208
DYL1_DROME	ctp	physical	14605208
MBL_DROME	mbl	physical	14605208
DEFI8_DROME	CG11534	physical	22036573
PSA71_DROME	Prosalpha4	physical	22036573
YPL1_DROME	CG15309	physical	22036573
PSB3_DROME	Prosbeta3	physical	22036573
PSA6_DROME	Prosalpha1	physical	22036573
REF2P_DROME	ref(2)P	physical	25931115

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of REF2P_DROME

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphoproteome analysis of Drosophila melanogaster embryos."; Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.; J. Proteome Res. 7:1675-1682(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-447, AND MASSSPECTROMETRY.	18327897 [Abstract]