NRPE1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NRPE1_ARATH
• UniProt AC: Q5D869
• Protein Name: DNA-directed RNA polymerase V subunit 1
• Gene Name: NRPE1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 1976
• Subcellular Localization: Nucleus, nucleolus . Clustered within heterochromatic regions and colocalized with RDR2, DCL3, NRPD4, AGO4, and siRNAs within the nucleolus. Recruited to chromatin via its interaction with SUVH2.
• Protein Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase V involved in RNA-directed DNA methylation-dependent (RdDM) silencing of endogenous repeated sequences, including transposable elements. Also required for full erasure of methylation when the RNA trigger is withdrawn. Seems also involved in the synthesis of short-interfering RNAs (siRNA). Essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production. Involved in the maintenance of post-transcriptional RNA silencing..
• Protein Sequence: MEEESTSEILDGEIVGITFALASHHEICIQSISESAINHPSQLTNAFLGLPLEFGKCESCGATEPDKCEGHFGYIQLPVPIYHPAHVNELKQMLSLLCLKCLKIKKAKGTSGGLADRLLGVCCEEASQISIKDRASDGASYLELKLPSRSRLQPGCWNFLERYGYRYGSDYTRPLLAREVKEILRRIPEESRKKLTAKGHIPQEGYILEYLPVPPNCLSVPEASDGFSTMSVDPSRIELKDVLKKVIAIKSSRSGETNFESHKAEASEMFRVVDTYLQVRGTAKAARNIDMRYGVSKISDSSSSKAWTEKMRTLFIRKGSGFSSRSVITGDAYRHVNEVGIPIEIAQRITFEERVSVHNRGYLQKLVDDKLCLSYTQGSTTYSLRDGSKGHTELKPGQVVHRRVMDGDVVFINRPPTTHKHSLQALRVYVHEDNTVKINPLMCSPLSADFDGDCVHLFYPQSLSAKAEVMELFSVEKQLLSSHTGQLILQMGSDSLLSLRVMLERVFLDKATAQQLAMYGSLSLPPPALRKSSKSGPAWTVFQILQLAFPERLSCKGDRFLVDGSDLLKFDFGVDAMGSIINEIVTSIFLEKGPKETLGFFDSLQPLLMESLFAEGFSLSLEDLSMSRADMDVIHNLIIREISPMVSRLRLSYRDELQLENSIHKVKEVAANFMLKSYSIRNLIDIKSNSAITKLVQQTGFLGLQLSDKKKFYTKTLVEDMAIFCKRKYGRISSSGDFGIVKGCFFHGLDPYEEMAHSIAAREVIVRSSRGLAEPGTLFKNLMAVLRDIVITNDGTVRNTCSNSVIQFKYGVDSERGHQGLFEAGEPVGVLAATAMSNPAYKAVLDSSPNSNSSWELMKEVLLCKVNFQNTTNDRRVILYLNECHCGKRFCQENAACTVRNKLNKVSLKDTAVEFLVEYRKQPTISEIFGIDSCLHGHIHLNKTLLQDWNISMQDIHQKCEDVINSLGQKKKKKATDDFKRTSLSVSECCSFRDPCGSKGSDMPCLTFSYNATDPDLERTLDVLCNTVYPVLLEIVIKGDSRICSANIIWNSSDMTTWIRNRHASRRGEWVLDVTVEKSAVKQSGDAWRVVIDSCLSVLHLIDTKRSIPYSVKQVQELLGLSCAFEQAVQRLSASVRMVSKGVLKEHIILLANNMTCSGTMLGFNSGGYKALTRSLNIKAPFTEATLIAPRKCFEKAAEKCHTDSLSTVVGSCSWGKRVDVGTGSQFELLWNQKETGLDDKEETDVYSFLQMVISTTNADAFVSSPGFDVTEEEMAEWAESPERDSALGEPKFEDSADFQNLHDEGKPSGANWEKSSSWDNGCSGGSEWGVSKSTGGEANPESNWEKTTNVEKEDAWSSWNTRKDAQESSKSDSGGAWGIKTKDADADTTPNWETSPAPKDSIVPENNEPTSDVWGHKSVSDKSWDKKNWGTESAPAAWGSTDAAVWGSSDKKNSETESDAAAWGSRDKNNSDVGSGAGVLGPWNKKSSETESNGATWGSSDKTKSGAAAWNSWDKKNIETDSEPAAWGSQGKKNSETESGPAAWGAWDKKKSETEPGPAGWGMGDKKNSETELGPAAMGNWDKKKSDTKSGPAAWGSTDAAAWGSSDKNNSETESDAAAWGSRNKKTSEIESGAGAWGSWGQPSPTAEDKDTNEDDRNPWVSLKETKSREKDDKERSQWGNPAKKFPSSGGWSNGGGADWKGNRNHTPRPPRSEDNLAPMFTATRQRLDSFTSEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTKPRPSGNRDRNNQDATPPGEEQSQPPNQSIGNGGDDFQTQTQSQSPSQTRAQSPSQAQAQSPSQTQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSPSQTQTQSPSQTQAQAQSPSSQSPSQTQT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
733	Phosphorylation	KRKYGRISSSGDFGI HHHHCCCCCCCCCCE	19.65	25561503
734	Phosphorylation	RKYGRISSSGDFGIV HHHCCCCCCCCCCEE	35.65	25561503
735	Phosphorylation	KYGRISSSGDFGIVK HHCCCCCCCCCCEEE	35.27	25561503
768	Phosphorylation	AREVIVRSSRGLAEP HHHHHHHHCCCCCCC	17.31	19880383
769	Phosphorylation	REVIVRSSRGLAEPG HHHHHHHCCCCCCCC	21.58	19880383
800	Phosphorylation	NDGTVRNTCSNSVIQ CCCCCCCCCCCCEEE	13.52	27531888
814	Phosphorylation	QFKYGVDSERGHQGL EEEECCCCCCCCCCH	27.13	27531888
1396	Phosphorylation	TTPNWETSPAPKDSI CCCCCCCCCCCCCCC	13.93	25561503
1570	Phosphorylation	GMGDKKNSETELGPA CCCCCCCCCCCCCCC	55.57	25561503

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NRPE1_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NRPE1_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NRPE1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AGO4_ARATH	AGO4	physical	17938239
AGO2_HUMAN	AGO2	physical	17938239
AGO4_ARATH	AGO4	physical	16839879
AGO4_ARATH	AGO4	physical	19410546
NRPBC_ARATH	NRPB12	physical	25561521
RPE5A_ARATH	NRPE5	physical	25561521
NRPB3_ARATH	NRPB3	physical	25561521
NRPBB_ARATH	NRPB11	physical	25561521
RPD3B_ARATH	NRPE3B	physical	25561521
RPB9A_ARATH	NRPB9A	physical	25561521
RPB9B_ARATH	NRPB9B	physical	25561521
NRPD2_ARATH	NRPD2A	physical	25561521
NRPBA_ARATH	NRPB10	physical	25561521
RPE5C_ARATH	RPB5E	physical	25561521
RPD6A_ARATH	NRPB6A	physical	25561521
NRPD4_ARATH	NRPD4	physical	25561521
NRPE7_ARATH	NRPE7	physical	25561521
RPB8A_ARATH	NRPB8A	physical	25561521
RPB6B_ARATH	NRPB6B	physical	25561521
AGO4_ARATH	AGO4	physical	25561521
DMS3_ARATH	DMS3	physical	25561521
ROS1_ARATH	DML1	genetic	25420628

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.