ROS1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ROS1_ARATH
• UniProt AC: Q9SJQ6
• Protein Name: Protein ROS1
• Gene Name: ROS1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 1393
• Subcellular Localization: Nucleus . Nucleus, nucleolus . Co-localizes in nucleoplasmic foci with APE1L and ZDP, two components of the DNA demethylase machinery.
• Protein Description: Bifunctional DNA glycosylase/lyase, which excises 5-methylcytosine (5-meC) and 5-hydroxymethylcytosine (5-hmeC), leaving an apyrimidinic (AP) site that is subsequently incised by the lyase activity. [PubMed: 25240767 Generates 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) as a primary 5-meC excision intermediate]
• Protein Sequence: MEKQRREESSFQQPPWIPQTPMKPFSPICPYTVEDQYHSSQLEERRFVGNKDMSGLDHLSFGDLLALANTASLIFSGQTPIPTRNTEVMQKGTEEVESLSSVSNNVAEQILKTPEKPKRKKHRPKVRREAKPKREPKPRAPRKSVVTDGQESKTPKRKYVRKKVEVSKDQDATPVESSAAVETSTRPKRLCRRVLDFEAENGENQTNGDIREAGEMESALQEKQLDSGNQELKDCLLSAPSTPKRKRSQGKRKGVQPKKNGSNLEEVDISMAQAAKRRQGPTCCDMNLSGIQYDEQCDYQKMHWLYSPNLQQGGMRYDAICSKVFSGQQHNYVSAFHATCYSSTSQLSANRVLTVEERREGIFQGRQESELNVLSDKIDTPIKKKTTGHARFRNLSSMNKLVEVPEHLTSGYCSKPQQNNKILVDTRVTVSKKKPTKSEKSQTKQKNLLPNLCRFPPSFTGLSPDELWKRRNSIETISELLRLLDINREHSETALVPYTMNSQIVLFGGGAGAIVPVTPVKKPRPRPKVDLDDETDRVWKLLLENINSEGVDGSDEQKAKWWEEERNVFRGRADSFIARMHLVQGDRRFTPWKGSVVDSVVGVFLTQNVSDHLSSSAFMSLASQFPVPFVPSSNFDAGTSSMPSIQITYLDSEETMSSPPDHNHSSVTLKNTQPDEEKDYVPSNETSRSSSEIAISAHESVDKTTDSKEYVDSDRKGSSVEVDKTDEKCRVLNLFPSEDSALTCQHSMVSDAPQNTERAGSSSEIDLEGEYRTSFMKLLQGVQVSLEDSNQVSPNMSPGDCSSEIKGFQSMKEPTKSSVDSSEPGCCSQQDGDVLSCQKPTLKEKGKKVLKEEKKAFDWDCLRREAQARAGIREKTRSTMDTVDWKAIRAADVKEVAETIKSRGMNHKLAERIQGFLDRLVNDHGSIDLEWLRDVPPDKAKEYLLSFNGLGLKSVECVRLLTLHHLAFPVDTNVGRIAVRLGWVPLQPLPESLQLHLLEMYPMLESIQKYLWPRLCKLDQKTLYELHYQMITFGKVFCTKSKPNCNACPMKGECRHFASAFASARLALPSTEKGMGTPDKNPLPLHLPEPFQREQGSEVVQHSEPAKKVTCCEPIIEEPASPEPETAEVSIADIEEAFFEDPEEIPTIRLNMDAFTSNLKKIMEHNKELQDGNMSSALVALTAETASLPMPKLKNISQLRTEHRVYELPDEHPLLAQLEKREPDDPCSYLLAIWTPGETADSIQPSVSTCIFQANGMLCDEETCFSCNSIKETRSQIVRGTILIPCRTAMRGSFPLNGTYFQVNEVFADHASSLNPINVPRELIWELPRRTVYFGTSVPTIFKGLSTEKIQACFWKGYVCVRGFDRKTRGPKPLIARLHFPASKLKGQQANLA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
242	Phosphorylation	CLLSAPSTPKRKRSQ HHHHCCCCCCCCCCC	31.60	23572148
575	Phosphorylation	VFRGRADSFIARMHL HHHCCHHHHHHHHHH	19.98	19880383
687	Phosphorylation	YVPSNETSRSSSEIA CCCCCCCCCCCCCEE	24.80	25561503
707	Phosphorylation	SVDKTTDSKEYVDSD CCCCCCCCHHHCCCC	25.87	25561503
761	Phosphorylation	QNTERAGSSSEIDLE CCCCCCCCCCCCCCC	29.70	25561503
762	Phosphorylation	NTERAGSSSEIDLEG CCCCCCCCCCCCCCC	30.95	25561503
763	Phosphorylation	TERAGSSSEIDLEGE CCCCCCCCCCCCCCC	40.02	25561503
901	Ubiquitination	KEVAETIKSRGMNHK HHHHHHHHHCCCCHH	41.33	17272265

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ROS1_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ROS1_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ROS1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ZDP_ARATH	AT3G14890	physical	22325353
XRCC1_ARATH	ATXRCC1	physical	23316050

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Ubiquitylation

"Multidimensional protein identification technology (MudPIT) analysisof ubiquitinated proteins in plants.";
Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
Mol. Cell. Proteomics 6:601-610(2007).
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-901, AND MASSSPECTROMETRY.
PubMed: 17272265