AGO4_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AGO4_ARATH
• UniProt AC: Q9ZVD5
• Protein Name: Protein argonaute 4
• Gene Name: AGO4
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 924
• Subcellular Localization: Nucleus, nucleolus . Nucleus, nucleoplasm . Nucleus, Cajal body . Also located in AB-bodies that are distincts from the Cajal bodies and immediately adjacent to the condensed 45S ribosomal DNA (rDNA) loci (PubMed:18266474). Colocalizes with AGO4 and
• Protein Description: Together with RDM3, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci. [PubMed: 21738482 Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be not required for CpG methylation. Required for the production and maintenance of retrotransposon SN1 and Copia and ribosomal 5S 25 nucleotide siRNAs specialized in gene silencing at chromatin level. Involved in de novo methylation of FWA gene and required for the maintenance of RNA-directed DNA methylation (RdDM) triggered by inverted repeat transgenes. Interacts with miRNA miR390 and miR172, targeting respectively TAS3 and AP2 mRNAs, and mediates cleavage of miRNA targets. Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO4 function in RNA silencing. Required for resistance to the bacterial pathogen P.syringae. Works independently of the RdDM pathway in mediating resistance to P.syringae. RdDM is involved in viral genome methylation as an epigenetic defense against geminiviruses]
• Protein Sequence: MDSTNGNGADLESANGANGSGVTEALPPPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLTNHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGEKTLFTYGALPSNKMDFSVVLEEVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKNFRVEISYAAKIPLQALANAMRGQESENSQEAIRVLDIILRQHAARQGCLLVRQSFFHNDPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPYSIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTVADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEKSRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETFPRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEEGNQFRRAPPMIRVENMFKDIQSKLPGVPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVTQCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSPGQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKKNGTEDDGIIKELLVDFYTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHHTKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADELQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPISVAQLPRLKDNVANSMFFC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
166	Phosphorylation	TRANGNGSPNGNESP CCCCCCCCCCCCCCC	21.13	23776212
172	Phosphorylation	GSPNGNESPSDGDRK CCCCCCCCCCCCCHH	33.43	23776212
174	Phosphorylation	PNGNESPSDGDRKRL CCCCCCCCCCCHHHC	64.60	23776212
636	Phosphorylation	AKLGGLNSMLSVERT HHCCCHHHHCCCCCC	26.58	30589143
890	Phosphorylation	FMKFEDQSETSSSHG CEECCCCCCCCCCCC	55.98	23776212
892	Phosphorylation	KFEDQSETSSSHGGI ECCCCCCCCCCCCCC	38.87	23776212
893	Phosphorylation	FEDQSETSSSHGGIT CCCCCCCCCCCCCCC	26.28	23776212
894	Phosphorylation	EDQSETSSSHGGITA CCCCCCCCCCCCCCC	33.50	23776212
895	Phosphorylation	DQSETSSSHGGITAP CCCCCCCCCCCCCCC	26.39	23776212
900	Phosphorylation	SSSHGGITAPGPISV CCCCCCCCCCCCCCH	30.03	23776212

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AGO4_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AGO4_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AGO4_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RDM1_ARATH	RDM1	physical	20410883
NRPE1_ARATH	NRPD1B	physical	19410546

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.