NHLC2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NHLC2_HUMAN
• UniProt AC: Q8NBF2
• Protein Name: NHL repeat-containing protein 2
• Gene Name: NHLRC2
• Organism: Homo sapiens (Human).
• Sequence Length: 726
• Protein Sequence: MAAPGGRGRSLSGLLPAQTSLEYALLDAVTQQEKDSLVYQYLQKVDGWEQDLSVPEFPEGLEWLNTEEPISVYKDLCGKIVVLDFFTYCCINCIHLLPDLHALEHTYSDKDGLLIIGVHSAKFPNEKVLDNIKSAVLRYNITHPMVNDADASLWQELEVSCWPTLVILGPRGNMLFSLIGEGHKDKLFLYTSIALKYYKDRGQIRDNKIGIKLYKDSLPPSPLLFPGKVTVDQVTDRLVIADTGHHRILVVWKNGQIQYSIGGPNPGRKDGIFSESTFNSPQGVAIMNNIIYVADTENHLIRKIDLEAEKVSTVAGIGIQGTDKEGGAKGEQQPISSPWDVVFGTSGSEVQRGDILWIAMAGTHQIWALLLDSGKLPKKNELTKGTCLRFAGSGNEENRNNAYPHKAGFAQPSGLSLASEDPWSCLFVADSESSTVRTVSLKDGAVKHLVGGERDPMNLFAFGDVDGVGINAKLQHPLGVTWDKKRNLLYVADSYNHKIKVVDPKTKNCTTLAGTGDTNNVTSSSFTESTFNEPGGLCIGENGELLYVADTNNHQIKVMDLETKMVSVLPIFRSENAVVDGPFLVEKQKTLPKLPKSAPSIRLSPVTACAGQTLQFKLRLDLPSGSKLTEGVSSCWFLTAEGNEWLLQGQIAAGDIENISSQPTISLQIPDDCLSLEAIVSVSVFLYYCSADSSACMMKAILFSQPLQITDTQQGCIAPVELRYVF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Methylation	-MAAPGGRGRSLSGL -CCCCCCCCCCCCCC	43.18	115484981
10	Phosphorylation	APGGRGRSLSGLLPA CCCCCCCCCCCCCCC	29.70	30278072
12	Phosphorylation	GGRGRSLSGLLPAQT CCCCCCCCCCCCCCC	28.74	30278072
19	Phosphorylation	SGLLPAQTSLEYALL CCCCCCCCHHHHHHH	37.07	23663014
20	Phosphorylation	GLLPAQTSLEYALLD CCCCCCCHHHHHHHH	13.71	23663014
23	Phosphorylation	PAQTSLEYALLDAVT CCCCHHHHHHHHHHH	14.08	23663014
39	Phosphorylation	QEKDSLVYQYLQKVD HHHHHHHHHHHHHCC	9.20	28796482
41	Phosphorylation	KDSLVYQYLQKVDGW HHHHHHHHHHHCCCC	8.07	28796482
127	Ubiquitination	SAKFPNEKVLDNIKS ECCCCCHHHHHHHHH	55.53	-
133	Ubiquitination	EKVLDNIKSAVLRYN HHHHHHHHHHHHHCC	38.32	21906983
133 (in isoform 1)	Ubiquitination	-	38.32	21906983
190	Phosphorylation	HKDKLFLYTSIALKY CCCCEEEEHHHHHHH	7.36	-
198	Phosphorylation	TSIALKYYKDRGQIR HHHHHHHHHHCCCCC	12.58	-
208	Ubiquitination	RGQIRDNKIGIKLYK CCCCCCCCCCEEEEC	46.74	-
212	Ubiquitination	RDNKIGIKLYKDSLP CCCCCCEEEECCCCC	40.32	-
215	Ubiquitination	KIGIKLYKDSLPPSP CCCEEEECCCCCCCC	51.80	-
221	Phosphorylation	YKDSLPPSPLLFPGK ECCCCCCCCCCCCCE	26.66	25850435
237	Methylation	TVDQVTDRLVIADTG EEEECCCEEEEECCC	22.84	115484989
303	Ubiquitination	TENHLIRKIDLEAEK CCCCEEEECCCCCEE	33.31	-
324	Ubiquitination	IGIQGTDKEGGAKGE EECCCCCCCCCCCCC	59.08	-
329 (in isoform 1)	Ubiquitination	-	67.28	21906983
329	Ubiquitination	TDKEGGAKGEQQPIS CCCCCCCCCCCCCCC	67.28	2190698
384	Ubiquitination	PKKNELTKGTCLRFA CCCCCCCCCCEEEEC	65.92	-
393	Phosphorylation	TCLRFAGSGNEENRN CEEEECCCCCHHCCC	34.71	28152594
403	Phosphorylation	EENRNNAYPHKAGFA HHCCCCCCCCCCCCC	14.16	27273156
406	Ubiquitination	RNNAYPHKAGFAQPS CCCCCCCCCCCCCCC	45.78	-
442	Ubiquitination	TVRTVSLKDGAVKHL EEEEEECCCCCEEEE	46.76	-
447	Ubiquitination	SLKDGAVKHLVGGER ECCCCCEEEECCCCC	30.59	-
505	2-Hydroxyisobutyrylation	KIKVVDPKTKNCTTL EEEEECCCCCCCEEE	68.61	-
567	Phosphorylation	DLETKMVSVLPIFRS ECCCCEEEEEEEECC	17.90	21406692
587	Ubiquitination	DGPFLVEKQKTLPKL ECCEEEEECCCCCCC	50.01	-
590	Phosphorylation	FLVEKQKTLPKLPKS EEEEECCCCCCCCCC	45.88	28060719
596	Ubiquitination	KTLPKLPKSAPSIRL CCCCCCCCCCCCCEE	70.46	-
600	Phosphorylation	KLPKSAPSIRLSPVT CCCCCCCCCEECCCE	21.76	27251275
604	Phosphorylation	SAPSIRLSPVTACAG CCCCCEECCCEECCC	13.89	27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NHLC2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NHLC2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NHLC2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BACH_HUMAN	ACOT7	physical	22863883
TKFC_HUMAN	DAK	physical	22863883
DPP3_HUMAN	DPP3	physical	22863883
PEPD_HUMAN	PEPD	physical	22863883
NHLC2_HUMAN	NHLRC2	physical	26472760
RB6I2_HUMAN	ERC1	physical	26472760

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.";
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.;
J. Proteome Res. 6:4150-4162(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-590, AND MASSSPECTROMETRY.
PubMed: 17924679

"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer.";
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.;
Cell 131:1190-1203(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-403, AND MASSSPECTROMETRY.
PubMed: 18083107