dbPTM

NFM_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFM_HUMAN
UniProt AC	P07197
Protein Name	Neurofilament medium polypeptide
Gene Name	NEFM
Organism	Homo sapiens (Human).
Sequence Length	916
Subcellular Localization
Protein Description	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber..
Protein Sequence	MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSMLAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRPPITISSKIQKPKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKGSRKEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPADEKKGGDKSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSYTLDSLG ------CCCCCCCCC	27.16	-
2	Phosphorylation	------MSYTLDSLG ------CCCCCCCCC	27.16	25072903
3	Phosphorylation	-----MSYTLDSLGN -----CCCCCCCCCC	15.26	25072903
4	Phosphorylation	----MSYTLDSLGNP ----CCCCCCCCCCH	19.44	25072903
7	Phosphorylation	-MSYTLDSLGNPSAY -CCCCCCCCCCHHHH	41.09	25072903
12	Phosphorylation	LDSLGNPSAYRRVTE CCCCCCHHHHHHHHC	41.76	25072903
14	Phosphorylation	SLGNPSAYRRVTETR CCCCHHHHHHHHCCH	11.86	25072903
18	O-linked_Glycosylation	PSAYRRVTETRSSFS HHHHHHHHCCHHHCC	29.81	8702840
18	O-linked_Glycosylation	PSAYRRVTETRSSFS HHHHHHHHCCHHHCC	29.81	8702840
18	Phosphorylation	PSAYRRVTETRSSFS HHHHHHHHCCHHHCC	29.81	26270265
20	Phosphorylation	AYRRVTETRSSFSRV HHHHHHCCHHHCCCC	26.87	26270265
21	Methylation	YRRVTETRSSFSRVS HHHHHCCHHHCCCCC	24.87	115484943
22	Phosphorylation	RRVTETRSSFSRVSG HHHHCCHHHCCCCCC	43.06	26270265
23	Phosphorylation	RVTETRSSFSRVSGS HHHCCHHHCCCCCCC	24.77	28355574
25	Phosphorylation	TETRSSFSRVSGSPS HCCHHHCCCCCCCCC	33.45	26270265
26	Methylation	ETRSSFSRVSGSPSS CCHHHCCCCCCCCCC	25.30	97770723
28	O-linked_Glycosylation	RSSFSRVSGSPSSGF HHHCCCCCCCCCCCC	32.23	30379171
28	Phosphorylation	RSSFSRVSGSPSSGF HHHCCCCCCCCCCCC	32.23	28348404
30	Phosphorylation	SFSRVSGSPSSGFRS HCCCCCCCCCCCCCC	17.69	29666759
32	Phosphorylation	SRVSGSPSSGFRSQS CCCCCCCCCCCCCCC	44.80	25307156
33	O-linked_Glycosylation	RVSGSPSSGFRSQSW CCCCCCCCCCCCCCC	45.39	-
33	O-linked_Glycosylation	RVSGSPSSGFRSQSW CCCCCCCCCCCCCCC	45.39	8702840
33	Phosphorylation	RVSGSPSSGFRSQSW CCCCCCCCCCCCCCC	45.39	27732954
37	Phosphorylation	SPSSGFRSQSWSRGS CCCCCCCCCCCCCCC	26.72	27732954
39	Phosphorylation	SSGFRSQSWSRGSPS CCCCCCCCCCCCCCC	28.41	27732954
41	Phosphorylation	GFRSQSWSRGSPSTV CCCCCCCCCCCCCCC	31.82	27732954
42	Methylation	FRSQSWSRGSPSTVS CCCCCCCCCCCCCCC	43.27	16186007
44	Phosphorylation	SQSWSRGSPSTVSSS CCCCCCCCCCCCCHH	17.99	25307156
46	Phosphorylation	SWSRGSPSTVSSSYK CCCCCCCCCCCHHHH	43.04	27732954
47	O-linked_Glycosylation	WSRGSPSTVSSSYKR CCCCCCCCCCHHHHH	28.00	23301498
47	O-linked_Glycosylation	WSRGSPSTVSSSYKR CCCCCCCCCCHHHHH	28.00	8344946
47	Phosphorylation	WSRGSPSTVSSSYKR CCCCCCCCCCHHHHH	28.00	27251275
49	O-linked_Glycosylation	RGSPSTVSSSYKRSM CCCCCCCCHHHHHHC	17.41	30379171
49	Phosphorylation	RGSPSTVSSSYKRSM CCCCCCCCHHHHHHC	17.41	27732954
50	O-linked_Glycosylation	GSPSTVSSSYKRSML CCCCCCCHHHHHHCH	33.09	30379171
50	Phosphorylation	GSPSTVSSSYKRSML CCCCCCCHHHHHHCH	33.09	25307156
51	O-linked_Glycosylation	SPSTVSSSYKRSMLA CCCCCCHHHHHHCHH	27.45	30379171
51	Phosphorylation	SPSTVSSSYKRSMLA CCCCCCHHHHHHCHH	27.45	29449344
54	Methylation	TVSSSYKRSMLAPRL CCCHHHHHHCHHHHH	21.04	115367679
55	Phosphorylation	VSSSYKRSMLAPRLA CCHHHHHHCHHHHHH	17.62	24719451
73	Phosphorylation	AMLSSAESSLDFSQS HHHHHHHHHCCHHHC	35.60	-
99	Phosphorylation	GDYKLSRSNEKEQLQ CCCCCCCCCHHHHHC	46.06	24076635
111	Methylation	QLQGLNDRFAGYIEK HHCCCHHHHHHHHHH	23.42	115484919
118	Ubiquitination	RFAGYIEKVHYLEQQ HHHHHHHHHHHHHHH	25.42	21890473
118	Ubiquitination	RFAGYIEKVHYLEQQ HHHHHHHHHHHHHHH	25.42	21890473
175	Phosphorylation	KAQVQLDSDHLEEDI HHHHHCCCHHHHHHH	35.21	29449344
214	Phosphorylation	RKDIEEASLVKVELD HHHHHHHHCEEHHHH	36.65	24719451
226	Phosphorylation	ELDKKVQSLQDEVAF HHHHHHHHHHHHHHH	30.87	24076635
259	Ubiquitination	SHITVERKDYLKTDI HCCEEECHHHHHCHH	36.90	21890473
259	Ubiquitination	SHITVERKDYLKTDI HCCEEECHHHHHCHH	36.90	21890473
261	Phosphorylation	ITVERKDYLKTDIST CEEECHHHHHCHHHH	17.09	29083192
263	Ubiquitination	VERKDYLKTDISTAL EECHHHHHCHHHHHH	37.61	21890473
263	Ubiquitination	VERKDYLKTDISTAL EECHHHHHCHHHHHH	37.61	21890473
264	Phosphorylation	ERKDYLKTDISTALK ECHHHHHCHHHHHHH	35.65	29083192
267	Phosphorylation	DYLKTDISTALKEIR HHHHCHHHHHHHHHH	15.50	29083192
268	Phosphorylation	YLKTDISTALKEIRS HHHCHHHHHHHHHHH	36.02	29083192
271	Ubiquitination	TDISTALKEIRSQLE CHHHHHHHHHHHHHH	48.94	21890473
271	Ubiquitination	TDISTALKEIRSQLE CHHHHHHHHHHHHHH	48.94	21890473
279	Phosphorylation	EIRSQLESHSDQNMH HHHHHHHHCCCCCHH	36.56	29449344
281	Phosphorylation	RSQLESHSDQNMHQA HHHHHHCCCCCHHHH	50.61	29449344
296	Phosphorylation	EEWFKCRYAKLTEAA HHHHHHHHHHHHHHH	19.98	28331001
300	Phosphorylation	KCRYAKLTEAAEQNK HHHHHHHHHHHHHCH	23.91	28331001
320	Phosphorylation	AKEEIAEYRRQLQSK HHHHHHHHHHHHHHC	11.32	-
328	Phosphorylation	RRQLQSKSIELESVR HHHHHHCCCCHHHHH	26.92	24076635
333	Phosphorylation	SKSIELESVRGTKES HCCCCHHHHHCCHHH	29.73	-
346	Phosphorylation	ESLERQLSDIEERHN HHHHHHHHHHHHHHC	28.36	23822953
382	Methylation	WEMARHLREYQDLLN HHHHHHHHHHHHHHC	34.16	-
384	Phosphorylation	MARHLREYQDLLNVK HHHHHHHHHHHHCHH	10.82	21253578
401	Phosphorylation	LDIEIAAYRKLLEGE HHHHHHHHHHHHCCC	10.16	-
410	Phosphorylation	KLLEGEETRFSTFAG HHHCCCCCCCCCCCC	32.48	21815630
413	Phosphorylation	EGEETRFSTFAGSIT CCCCCCCCCCCCCCC	21.23	23684312
414	Phosphorylation	GEETRFSTFAGSITG CCCCCCCCCCCCCCC	18.13	23684312
418	Phosphorylation	RFSTFAGSITGPLYT CCCCCCCCCCCCCCC	17.34	23684312
420	Phosphorylation	STFAGSITGPLYTHR CCCCCCCCCCCCCCC	34.15	23684312
424	Phosphorylation	GSITGPLYTHRPPIT CCCCCCCCCCCCCEE	12.02	23684312
425	Phosphorylation	SITGPLYTHRPPITI CCCCCCCCCCCCEEE	21.06	23684312
431	O-linked_Glycosylation	YTHRPPITISSKIQK CCCCCCEEECCCCCC	22.15	8344946
431	O-linked_Glycosylation	YTHRPPITISSKIQK CCCCCCEEECCCCCC	22.15	8344946
431	Phosphorylation	YTHRPPITISSKIQK CCCCCCEEECCCCCC	22.15	23684312
433	Phosphorylation	HRPPITISSKIQKPK CCCCEEECCCCCCCC	19.54	23684312
434	Phosphorylation	RPPITISSKIQKPKV CCCEEECCCCCCCCC	29.90	23684312
445	Ubiquitination	KPKVEAPKLKVQHKF CCCCCCCCCHHCHHH	68.91	-
451	Ubiquitination	PKLKVQHKFVEEIIE CCCHHCHHHHHHHHH	33.73	21890473
451	Ubiquitination	PKLKVQHKFVEEIIE CCCHHCHHHHHHHHH	33.73	21890473
460	Phosphorylation	VEEIIEETKVEDEKS HHHHHHHHCCCCCHH	27.83	30576142
467	Phosphorylation	TKVEDEKSEMEEALT HCCCCCHHHHHHHHH	39.88	20068231
474	Phosphorylation	SEMEEALTAITEELA HHHHHHHHHHHHHHH	24.06	27732954
477	Phosphorylation	EEALTAITEELAVSM HHHHHHHHHHHHHHH	22.42	27732954
483	Phosphorylation	ITEELAVSMKEEKKE HHHHHHHHHHHHHHH	20.66	27732954
511	Phosphorylation	EEVAAKKSPVKATAP HHHHHHHCCCCCCCC	33.93	17287340
545	Phosphorylation	EEDEGAKSDQAEEGG HHCCCCCCCCHHCCC	34.13	18510355
553	Phosphorylation	DQAEEGGSEKEGSSE CCHHCCCCCCCCCCC	57.25	18510355
558	Phosphorylation	GGSEKEGSSEKEEGE CCCCCCCCCCCCCCC	36.09	25022875
559	Phosphorylation	GSEKEGSSEKEEGEQ CCCCCCCCCCCCCCC	64.64	25022875
571	Phosphorylation	GEQEEGETEAEAEGE CCCCCCCCHHHHHHH	51.88	26657352
592	Phosphorylation	EKKVEEKSEEVATKE HHHHHHHHHHHCCHH	42.12	29449344
615	Phosphorylation	EKPEKAKSPVPKSPV CCCHHCCCCCCCCCC	35.67	22617229
620	Phosphorylation	AKSPVPKSPVEEKGK CCCCCCCCCCHHCCC	28.27	22617229
628	Phosphorylation	PVEEKGKSPVPKSPV CCHHCCCCCCCCCCC	39.54	22617229
633	Phosphorylation	GKSPVPKSPVEEKGK CCCCCCCCCCHHCCC	28.27	22617229
641	Phosphorylation	PVEEKGKSPVPKSPV CCHHCCCCCCCCCCC	39.54	22617229
646	Phosphorylation	GKSPVPKSPVEEKGK CCCCCCCCCCHHCCC	28.27	22617229
653	Acetylation	SPVEEKGKSPVPKSP CCCHHCCCCCCCCCC	63.76	7675151
654	Phosphorylation	PVEEKGKSPVPKSPV CCHHCCCCCCCCCCC	39.54	22617229
659	Phosphorylation	GKSPVPKSPVEEKGK CCCCCCCCCCHHCCC	28.27	22617229
667	Phosphorylation	PVEEKGKSPVSKSPV CCHHCCCCCCCCCCH	38.92	25072903
670	Phosphorylation	EKGKSPVSKSPVEEK HCCCCCCCCCCHHHH	30.69	24972180
672	Phosphorylation	GKSPVSKSPVEEKAK CCCCCCCCCHHHHCC	27.03	22617229
680	Phosphorylation	PVEEKAKSPVPKSPV CHHHHCCCCCCCCHH	35.67	22617229
685	Phosphorylation	AKSPVPKSPVEEAKS CCCCCCCCHHHHHHH	28.27	22617229
693	Acetylation	PVEEAKSKAEVGKGE HHHHHHHHHCCCCCC	48.11	24232269
698	Acetylation	KSKAEVGKGEQKEEE HHHHCCCCCCHHHHH	65.53	24232281
736	Phosphorylation	PEKKKAESPVKEEAV CCHHHCCCCCCHHHH	39.59	22617229
748	Phosphorylation	EAVAEVVTITKSVKV HHHHHEEEEECEEEE	28.27	-
750	Phosphorylation	VAEVVTITKSVKVHL HHHEEEEECEEEEEE	14.11	-
751	Ubiquitination	AEVVTITKSVKVHLE HHEEEEECEEEEEEH	49.77	21890473
751	Ubiquitination	AEVVTITKSVKVHLE HHEEEEECEEEEEEH	49.77	21890473
783	Phosphorylation	KAGGEGGSEEEGSDK HCCCCCCCCCCCCCC	53.33	28857561
788	Phosphorylation	GGSEEEGSDKGAKGS CCCCCCCCCCCCCCC	39.15	17287340
821	Phosphorylation	QETKEKGSGREEEKG HHHHHCCCCCHHHCC	45.83	27251275
831	Phosphorylation	EEEKGVVTNGLDLSP HHHCCEECCCCCCCH	22.59	24117733
837	Phosphorylation	VTNGLDLSPADEKKG ECCCCCCCHHHHCCC	20.08	22617229
862	Phosphorylation	TKTVEKITSEGGDGA EEEEEEHHCCCCCCC	31.62	29449344
863	O-linked_Glycosylation	KTVEKITSEGGDGAT EEEEEHHCCCCCCCC	37.40	30379171
863	Phosphorylation	KTVEKITSEGGDGAT EEEEEHHCCCCCCCC	37.40	29449344
870	Phosphorylation	SEGGDGATKYITKSV CCCCCCCCEEEEEEE	29.88	29759185
871	Ubiquitination	EGGDGATKYITKSVT CCCCCCCEEEEEEEE	33.84	21890473
871	Ubiquitination	EGGDGATKYITKSVT CCCCCCCEEEEEEEE	33.84	21890473
872	Phosphorylation	GGDGATKYITKSVTV CCCCCCEEEEEEEEE	14.96	21406692
874	Phosphorylation	DGATKYITKSVTVTQ CCCCEEEEEEEEEEE	17.74	21406692
875	Ubiquitination	GATKYITKSVTVTQK CCCEEEEEEEEEEEE	33.11	21890473
875	Ubiquitination	GATKYITKSVTVTQK CCCEEEEEEEEEEEE	33.11	21890473
876	Phosphorylation	ATKYITKSVTVTQKV CCEEEEEEEEEEEEH	17.86	21406692
878	Phosphorylation	KYITKSVTVTQKVEE EEEEEEEEEEEEHHH	25.56	21406692
880	Phosphorylation	ITKSVTVTQKVEEHE EEEEEEEEEEHHHCH	17.20	21406692
904	Phosphorylation	TKKVEKVTSHAIVKE CCCCHHHCCCHHHHH	26.34	20873877
905	Phosphorylation	KKVEKVTSHAIVKEV CCCHHHCCCHHHHHH	17.57	20873877

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	STUB1	Q9UNE7	PMID:21219885

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFM_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFM_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CALD1_HUMAN	CALD1	physical	26496610
SSXT_HUMAN	SS18	physical	26496610
DNJA2_HUMAN	DNAJA2	physical	26496610
STML2_HUMAN	STOML2	physical	26496610
RPR1B_HUMAN	RPRD1B	physical	26496610
TDIF1_HUMAN	DNTTIP1	physical	26496610
DESM_HUMAN	DES	physical	28514442
AINX_HUMAN	INA	physical	28514442
NFL_HUMAN	NEFL	physical	28514442
NEST_HUMAN	NES	physical	28514442
FBXW5_HUMAN	FBXW5	physical	28514442
KIF7_HUMAN	KIF7	physical	28514442
GFAP_HUMAN	GFAP	physical	28514442
PLEC_HUMAN	PLEC	physical	28514442
CCHCR_HUMAN	CCHCR1	physical	28514442
GAN_HUMAN	GAN	physical	28514442
GOGA2_HUMAN	GOLGA2	physical	28514442

Drug and Disease Associations

Kegg Disease
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFM_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Global proteomic profiling of phosphopeptides using electron transferdissociation tandem mass spectrometry."; Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.; Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-511; SER-615; SER-620;SER-654; SER-659; SER-680; SER-685; SER-736; SER-783 AND SER-788, ANDMASS SPECTROMETRY.	17287340 [Abstract]
"Phosphoproteomic analysis of synaptosomes from human cerebralcortex."; DeGiorgis J.A., Jaffe H., Moreira J.E., Carlotti C.G. Jr., Leite J.P.,Pant H.C., Dosemeci A.; J. Proteome Res. 4:306-315(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-615; SER-620; SER-628;SER-633; SER-667; SER-672; SER-680; SER-685 AND SER-736, AND MASSSPECTROMETRY.	15822905 [Abstract]