NCK2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NCK2_MOUSE
• UniProt AC: O55033
• Protein Name: Cytoplasmic protein NCK2
• Gene Name: Nck2
• Organism: Mus musculus (Mouse).
• Sequence Length: 380
• Subcellular Localization: Cytoplasm. Endoplasmic reticulum.
• Protein Description: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling (By similarity)..
• Protein Sequence: MTEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKPSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSFNGQIGWFPSNYVLEEADEAAAEAPSFLSLRRGTALSNGQGARVLHVVQTLYPFSSVTEEELSFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHTPQISYTGPSASGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGRNKHFKVQLVDSVYCIGQRRFHSMDELVEHYKKAPIFTSEHGEKLYLVRALQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MTEEVIVIA ------CCEEEEEEE	40.63	-
35	Phosphorylation	RLWLLDDSKTWWRVR EEEEECCCCCHHHHC	31.48	26745281
37	Phosphorylation	WLLDDSKTWWRVRNA EEECCCCCHHHHCCC	33.92	22817900
50	Phosphorylation	NAANRTGYVPSNYVE CCCCCCCCCCCHHHH	14.47	25177544
55	Phosphorylation	TGYVPSNYVERKNSL CCCCCCHHHHHCCCC	14.18	29514104
61	Phosphorylation	NYVERKNSLKKGSLV HHHHHCCCCCCCCHH	44.60	23737553
66	Phosphorylation	KNSLKKGSLVKNLKD CCCCCCCCHHHHHHH	38.58	25338131
72	Ubiquitination	GSLVKNLKDTLGLGK CCHHHHHHHHCCCCC	59.05	22790023
74	Phosphorylation	LVKNLKDTLGLGKTR HHHHHHHHCCCCCCC	22.57	22817900
90	Phosphorylation	KPSARDASPTPSTDA CCCCCCCCCCCCCCC	33.33	25521595
92	Phosphorylation	SARDASPTPSTDAEY CCCCCCCCCCCCCCC	27.63	27742792
94	Phosphorylation	RDASPTPSTDAEYPA CCCCCCCCCCCCCCC	41.20	27087446
95	Phosphorylation	DASPTPSTDAEYPAN CCCCCCCCCCCCCCC	40.19	21082442
99	Phosphorylation	TPSTDAEYPANGSGA CCCCCCCCCCCCCCC	14.90	25619855
104	Phosphorylation	AEYPANGSGADRIYD CCCCCCCCCCCCEEE	30.52	25619855
110	Phosphorylation	GSGADRIYDLNIPAF CCCCCCEEECCCCCE	18.44	18515860
139	Phosphorylation	LVKGSRVTVMEKCSD EECCCEEEEEEECCC	17.02	19854140
152	Phosphorylation	SDGWWRGSFNGQIGW CCCEEECCCCCEEEE	13.63	27180971
181	Phosphorylation	AEAPSFLSLRRGTAL HHCCCCCEECCCCCC	20.16	-
186	Phosphorylation	FLSLRRGTALSNGQG CCEECCCCCCCCCCC	23.71	29514104
189	Phosphorylation	LRRGTALSNGQGARV ECCCCCCCCCCCCEE	36.13	26824392
251	Phosphorylation	VGLVPKNYVVVLSDG CEEECCCEEEEECCC	10.76	29514104
316	Phosphorylation	LIRDSESSPSDFSVS EEECCCCCCCCEEEE	25.11	26370283
342	Phosphorylation	VQLVDSVYCIGQRRF EEEECCEEECCCCCC	5.15	22345495
351	Phosphorylation	IGQRRFHSMDELVEH CCCCCCCCHHHHHHH	25.80	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NCK2_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NCK2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NCK2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ABI1_MOUSE	Abi1	physical	11557983
AXIN1_MOUSE	Axin1	physical	11557983
DYN1_MOUSE	Dnm1	physical	11557983
HNRPK_MOUSE	Hnrnpk	physical	11557983
SRBS1_MOUSE	Sorbs1	physical	11557983

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain.";
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
J. Proteome Res. 7:311-318(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-342, AND MASSSPECTROMETRY.
PubMed: 18034455