MOCS3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MOCS3_HUMAN
• UniProt AC: O95396
• Protein Name: Adenylyltransferase and sulfurtransferase MOCS3 {ECO:0000255|HAMAP-Rule:MF_03049}
• Gene Name: MOCS3 {ECO:0000255|HAMAP-Rule:MF_03049}
• Organism: Homo sapiens (Human).
• Sequence Length: 460
• Subcellular Localization: Cytoplasm .
• Protein Description: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions..
• Protein Sequence: MASREEVLALQAEVAQREEELNSLKQKLASALLAEQEPQPERLVPVSPLPPKAALSRDEILRYSRQLVLPELGVHGQLRLGTACVLIVGCGGLGCPLAQYLAAAGVGRLGLVDYDVVEMSNLARQVLHGEALAGQAKAFSAAASLRRLNSAVECVPYTQALTPATALDLVRRYDVVADCSDNVPTRYLVNDACVLAGRPLVSASALRFEGQITVYHYDGGPCYRCIFPQPPPAETVTNCADGGVLGVVTGVLGCLQALEVLKIAAGLGPSYSGSLLLFDALRGHFRSIRLRSRRLDCAACGERPTVTDLLDYEAFCGSSATDKCRSLQLLSPEERVSVTDYKRLLDSGAFHLLLDVRPQVEVDICRLPHALHIPLKHLERRDAESLKLLKEAIWEEKQGTQEGAAVPIYVICKLGNDSQKAVKILQSLSAAQELDPLTVRDVVGGLMAWAAKIDGTFPQY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Ubiquitination	EEELNSLKQKLASAL HHHHHHHHHHHHHHH	45.25	22817900
27	Ubiquitination	ELNSLKQKLASALLA HHHHHHHHHHHHHHH	44.42	21906983
47	Phosphorylation	PERLVPVSPLPPKAA CCCEEECCCCCCCCC	18.04	25159151
52	Ubiquitination	PVSPLPPKAALSRDE ECCCCCCCCCCCHHH	44.33	27667366
63	Phosphorylation	SRDEILRYSRQLVLP CHHHHHHHHHHHHCH	12.54	27174698
64	Phosphorylation	RDEILRYSRQLVLPE HHHHHHHHHHHHCHH	13.71	27174698
137	Ubiquitination	EALAGQAKAFSAAAS HHHHHHHHHHHHHHH	41.92	22817900
144	Phosphorylation	KAFSAAASLRRLNSA HHHHHHHHHHHHHHC	20.06	28857561
150	Phosphorylation	ASLRRLNSAVECVPY HHHHHHHHCCCCCCC	37.98	28857561
187	Phosphorylation	SDNVPTRYLVNDACV CCCCCCCCEECCHHH	19.92	-
312	Phosphorylation	TVTDLLDYEAFCGSS CHHHHCCHHHHCCCC	14.77	27642862
326	Phosphorylation	SATDKCRSLQLLSPE CHHHHHHHCCCCCHH	29.91	27499020
331	Phosphorylation	CRSLQLLSPEERVSV HHHCCCCCHHHCCCH	38.57	21815630
342	Ubiquitination	RVSVTDYKRLLDSGA CCCHHCHHHHHHCCC	39.10	22817900
376	Ubiquitination	HALHIPLKHLERRDA CHHCCCHHHHHHCCH	40.57	-
387	Ubiquitination	RRDAESLKLLKEAIW HCCHHHHHHHHHHHH	62.14	22817900
390	Ubiquitination	AESLKLLKEAIWEEK HHHHHHHHHHHHHHH	56.38	22817900
397	Ubiquitination	KEAIWEEKQGTQEGA HHHHHHHHCCCCCCC	42.38	29967540
400	Phosphorylation	IWEEKQGTQEGAAVP HHHHHCCCCCCCEEE	22.43	29759185
413	Ubiquitination	VPIYVICKLGNDSQK EEEEEEEECCCCHHH	48.59	-
420	Ubiquitination	KLGNDSQKAVKILQS ECCCCHHHHHHHHHH	60.13	29967540
423	Ubiquitination	NDSQKAVKILQSLSA CCHHHHHHHHHHCCH	42.43	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MOCS3_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MOCS3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MOCS3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KLC2_HUMAN	KLC2	physical	28514442
FXL18_HUMAN	FBXL18	physical	28514442
HERC4_HUMAN	HERC4	physical	28514442
DYHC2_HUMAN	DYNC2H1	physical	28514442
SACS_HUMAN	SACS	physical	28514442
HSP76_HUMAN	HSPA6	physical	28514442
VPS16_HUMAN	VPS16	physical	28514442
LIN9_HUMAN	LIN9	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.