MITF_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MITF_MOUSE
• UniProt AC: Q08874
• Protein Name: Microphthalmia-associated transcription factor
• Gene Name: Mitf
• Organism: Mus musculus (Mouse).
• Sequence Length: 526
• Subcellular Localization: Nucleus .
• Protein Description: Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). [PubMed: 23207919 Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1 Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.]
• Protein Sequence: MQSESGIVADFEVGEEFHEEPKTYYELKSQPLKSSSSAEHSGASKPPLSSSTMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVAVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRHQVKQYLSTTLANKHASQVLSSPCPNQPGDHAMPPVPGSSAPNSPMAMLTLNSNCEKEAFYKFEEQSRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYSNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKDLENRQKKLEHANRHLLLRVQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQELVQHQADLTCTTTLDLTDGTITFTNNLGTMPESSPAYSIPRKMGSNLEDILMDDALSPVGVTDPLLSSVSPGASKTSSRRSSMSAEETEHAC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7 (in isoform 9)	Phosphorylation	-	5.90	27180971
7 (in isoform 8)	Phosphorylation	-	5.90	27180971
146	Phosphorylation	VKQYLSTTLANKHAS HHHHHHHHHHHHHHH	22.09	29514104
175	Phosphorylation	AMPPVPGSSAPNSPM CCCCCCCCCCCCCCC	19.77	25338131
180	Phosphorylation	PGSSAPNSPMAMLTL CCCCCCCCCCEEEEC	18.26	22817900
280	Phosphorylation	PGLTISNSCPANLPN CCCEECCCCCCCCCC	18.06	-
289	Sumoylation	PANLPNIKRELTACI CCCCCCHHCCEEEEE	46.60	-
336	Phosphorylation	DRIKELGTLIPKSND HHHHHHHHCCCCCCC	33.85	-
357	Phosphorylation	KGTILKASVDYIRKL HHHHHHHHHHHHHHH	17.59	-
360	Phosphorylation	ILKASVDYIRKLQRE HHHHHHHHHHHHHHH	10.64	-
405	Phosphorylation	QARAHGLSLIPSTGL HHHHCCCCCCCCCCC	28.92	22817900
409	Phosphorylation	HGLSLIPSTGLCSPD CCCCCCCCCCCCCHH	28.28	25159016
410	Phosphorylation	GLSLIPSTGLCSPDL CCCCCCCCCCCCHHH	28.90	25159016
414	Phosphorylation	IPSTGLCSPDLVNRI CCCCCCCCHHHHHHH	26.87	26824392
423	Sumoylation	DLVNRIIKQEPVLEN HHHHHHHHCCHHHHH	46.36	-
491	Phosphorylation	ILMDDALSPVGVTDP HHCCCCCCCCCCCCH	21.18	27180971
501	Phosphorylation	GVTDPLLSSVSPGAS CCCCHHHHCCCCCCC	36.05	20531401
502	Phosphorylation	VTDPLLSSVSPGASK CCCHHHHCCCCCCCC	26.95	20531401
504	Phosphorylation	DPLLSSVSPGASKTS CHHHHCCCCCCCCCC	21.40	27180971
508	Phosphorylation	SSVSPGASKTSSRRS HCCCCCCCCCCCCCC	42.24	20531401
510	Phosphorylation	VSPGASKTSSRRSSM CCCCCCCCCCCCCCC	29.46	23375375
511	Phosphorylation	SPGASKTSSRRSSMS CCCCCCCCCCCCCCC	26.11	23375375
515	Phosphorylation	SKTSSRRSSMSAEET CCCCCCCCCCCHHHH	29.32	29899451
516	Phosphorylation	KTSSRRSSMSAEETE CCCCCCCCCCHHHHC	18.23	30635358
518	Phosphorylation	SSRRSSMSAEETEHA CCCCCCCCHHHHCCC	34.18	27742792
522	Phosphorylation	SSMSAEETEHAC--- CCCCHHHHCCCC---	25.28	27742792

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
173	S	Phosphorylation	Kinase	MARK3	Q03141	PSP
180	S	Phosphorylation	Kinase	MAPK	-	Uniprot
307	S	Phosphorylation	Kinase	P38A	Q16539	PSP
307	S	Phosphorylation	Kinase	MAPK14	P47811	GPS
405	S	Phosphorylation	Kinase	GSK3	-	Uniprot
409	S	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
409	S	Phosphorylation	Kinase	P90RSK	P18653	PSP
516	S	Phosphorylation	Kinase	RPS6KA1	P18653	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
180	S	Phosphorylation	Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (By similarity).	-
180	S	Phosphorylation	Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome.	-
180	S	ubiquitylation	Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (By similarity).	-
180	S	ubiquitylation	Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome.	-
405	S	Phosphorylation	Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter (By similarity).	-
516	S	Phosphorylation	Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (By similarity).	-
516	S	ubiquitylation	Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MITF_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PAX6_MOUSE	Pax6	physical	20211142
PIAS3_MOUSE	Pias3	physical	20211142
PAX3_MOUSE	Pax3	genetic	15729346
HDAC7_MOUSE	Hdac7	physical	21324898
PSDE_MOUSE	Psmd14	physical	20058232

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-414, AND MASSSPECTROMETRY.
PubMed: 19367708