MFN1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MFN1_HUMAN
• UniProt AC: Q8IWA4
• Protein Name: Mitofusin-1
• Gene Name: MFN1
• Organism: Homo sapiens (Human).
• Sequence Length: 741
• Subcellular Localization: Mitochondrion outer membrane ; Multi-pass membrane protein .; Isoform 2: Cytoplasm .
• Protein Description: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. [PubMed: 12475957]
• Protein Sequence: MAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATVKNIMDSVNLAAEDKRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MAEPVSPLKHFVL --CCCCCCHHHHHHH	34.92	29255136
9	Ubiquitination	AEPVSPLKHFVLAKK CCCCCHHHHHHHHHH	38.08	22817900
9 (in isoform 2)	Ubiquitination	-	38.08	21890473
9 (in isoform 3)	Ubiquitination	-	38.08	21890473
15	Ubiquitination	LKHFVLAKKAITAIF HHHHHHHHHHHHHHH	38.28	29967540
17	Ubiquitination	HFVLAKKAITAIFDQ HHHHHHHHHHHHHHH	12.03	27667366
30	Phosphorylation	DQLLEFVTEGSHFVE HHHHHHHHHCCCCCE	39.69	29978859
33	Phosphorylation	LEFVTEGSHFVEATY HHHHHHCCCCCEEEC	13.91	29978859
39	Phosphorylation	GSHFVEATYKNPELD CCCCCEEECCCCCHH	22.86	29978859
40	Phosphorylation	SHFVEATYKNPELDR CCCCEEECCCCCHHC	18.80	29978859
47	Methylation	YKNPELDRIATEDDL CCCCCHHCCCCHHHH	32.82	115483199
61	Ubiquitination	LVEMQGYKDKLSIIG HHHCCCCHHHHHHHH	56.03	23000965
61 (in isoform 2)	Ubiquitination	-	56.03	21890473
61 (in isoform 3)	Ubiquitination	-	56.03	21890473
63	Ubiquitination	EMQGYKDKLSIIGEV HCCCCHHHHHHHHHH	39.73	23000965
63 (in isoform 2)	Ubiquitination	-	39.73	21890473
63 (in isoform 3)	Ubiquitination	-	39.73	21890473
77	Ubiquitination	VLSRRHMKVAFFGRT HHHHCCCEEEEEECC	25.04	-
82	Ubiquitination	HMKVAFFGRTSSGKS CCEEEEEECCCCCCC	26.04	21890473
86	Phosphorylation	AFFGRTSSGKSSVIN EEEECCCCCCCHHHH	50.02	-
89 (in isoform 1)	Ubiquitination	-	33.10	21890473
91 (in isoform 1)	Ubiquitination	-	5.16	21890473
122	Ubiquitination	VEGTDGDKAYLMTEG EECCCCCEEEEEECC	44.45	29967540
133	Ubiquitination	MTEGSDEKKSVKTVN EECCCCCHHHHHHHH	56.17	21906983
133 (in isoform 2)	Ubiquitination	-	56.17	21890473
133 (in isoform 3)	Ubiquitination	-	56.17	21890473
135	Phosphorylation	EGSDEKKSVKTVNQL CCCCCHHHHHHHHHH	39.66	21406692
138	Phosphorylation	DEKKSVKTVNQLAHA CCHHHHHHHHHHHHH	23.64	21406692
139	Ubiquitination	EKKSVKTVNQLAHAL CHHHHHHHHHHHHHH	3.40	23503661
150	Ubiquitination	AHALHMDKDLKAGCL HHHHHCCCCCCCCCE	58.34	23000965
150 (in isoform 2)	Ubiquitination	-	58.34	21890473
150 (in isoform 3)	Ubiquitination	-	58.34	21890473
153	Ubiquitination	LHMDKDLKAGCLVRV HHCCCCCCCCCEEEE	53.52	23000965
153 (in isoform 2)	Ubiquitination	-	53.52	21890473
153 (in isoform 3)	Ubiquitination	-	53.52	21890473
161 (in isoform 1)	Ubiquitination	-	6.91	21890473
164	Ubiquitination	LVRVFWPKAKCALLR EEEECCCHHHHHHHC	51.58	27667366
166	Ubiquitination	RVFWPKAKCALLRDD EECCCHHHHHHHCCC	26.60	29967540
178 (in isoform 1)	Ubiquitination	-	44.98	21890473
181 (in isoform 1)	Ubiquitination	-	38.20	21890473
187	Ubiquitination	PGTDVTTELDSWIDK CCCCCHHHHHHHHHH	41.01	22817900
189	Ubiquitination	TDVTTELDSWIDKFC CCCHHHHHHHHHHHC	35.38	22817900
198	Ubiquitination	WIDKFCLDADVFVLV HHHHHCCCCCEEEEE	41.73	23503661
218	Ubiquitination	TLMNTEKHFFHKVNE CCCCCHHHHHHHHHH	25.11	23503661
222	Ubiquitination	TEKHFFHKVNERLSK CHHHHHHHHHHHCCC	41.41	29967540
229	Ubiquitination	KVNERLSKPNIFILN HHHHHCCCCCEEEEC	46.83	23000965
229 (in isoform 2)	Ubiquitination	-	46.83	21890473
229 (in isoform 3)	Ubiquitination	-	46.83	21890473
248	Phosphorylation	ASASEPEYMEDVRRQ CCCCCCHHHHHHHHH	19.27	27642862
248	Ubiquitination	ASASEPEYMEDVRRQ CCCCCCHHHHHHHHH	19.27	21890473
249	Ubiquitination	SASEPEYMEDVRRQH CCCCCHHHHHHHHHH	3.19	22505724
250	Ubiquitination	ASEPEYMEDVRRQHM CCCCHHHHHHHHHHH	53.07	22505724
252	Ubiquitination	EPEYMEDVRRQHMER CCHHHHHHHHHHHHH	3.28	23503661
257 (in isoform 1)	Ubiquitination	-	2.35	21890473
286	Ubiquitination	RIFFVSAKEVLSARK CEEEEEHHHHHHHHH	40.63	23503661
286 (in isoform 3)	Ubiquitination	-	40.63	-
292	Ubiquitination	AKEVLSARKQKAQGM HHHHHHHHHHHHCCC	38.26	21963094
295	Ubiquitination	VLSARKQKAQGMPES HHHHHHHHHCCCCHH	45.79	22817900
334	Ubiquitination	CISQSAVKTKFEQHT HHCCHHHHHHHHHHH	45.79	22817900
336	Ubiquitination	SQSAVKTKFEQHTIR CCHHHHHHHHHHHHH	40.98	22817900
344	Ubiquitination	FEQHTIRAKQILATV HHHHHHHHHHHHHHH	12.31	23000965
345	Ubiquitination	EQHTIRAKQILATVK HHHHHHHHHHHHHHH	28.20	23503661
345 (in isoform 3)	Ubiquitination	-	28.20	-
352	Ubiquitination	KQILATVKNIMDSVN HHHHHHHHHHHHHCC	36.08	23000965
353	Ubiquitination	QILATVKNIMDSVNL HHHHHHHHHHHHCCH	30.40	21890473
357	Phosphorylation	TVKNIMDSVNLAAED HHHHHHHHCCHHHHH	8.77	27794612
365	Ubiquitination	VNLAAEDKRHYSVEE CCHHHHHHCCCCHHH	32.36	21906983
365 (in isoform 2)	Ubiquitination	-	32.36	21890473
365 (in isoform 3)	Ubiquitination	-	32.36	21890473
368	Phosphorylation	AAEDKRHYSVEERED HHHHHCCCCHHHCHH	20.60	27642862
391	Phosphorylation	RNQMNLLTLDVKKKI HHHCHHHHHHHHHHH	24.87	29083192
393 (in isoform 1)	Ubiquitination	-	44.98	21890473
395	Ubiquitination	NLLTLDVKKKIKEVT HHHHHHHHHHHHHHH	48.27	21906983
395 (in isoform 3)	Ubiquitination	-	48.27	21890473
396	Ubiquitination	LLTLDVKKKIKEVTE HHHHHHHHHHHHHHH	60.63	22505724
396 (in isoform 3)	Ubiquitination	-	60.63	-
397	Ubiquitination	LTLDVKKKIKEVTEE HHHHHHHHHHHHHHH	53.98	22505724
399	Malonylation	LDVKKKIKEVTEEVA HHHHHHHHHHHHHHH	55.82	30639696
399	Ubiquitination	LDVKKKIKEVTEEVA HHHHHHHHHHHHHHH	55.82	33845483
399 (in isoform 3)	Ubiquitination	-	55.82	-
408	Ubiquitination	VTEEVANKVSCAMTD HHHHHHHHHHHHHCH	26.28	29967540
423 (in isoform 1)	Ubiquitination	-	3.45	21890473
439	Ubiquitination	HPNPDVLKIYKSELN CCCHHHHHHHHHHHH	43.78	21906983
439 (in isoform 3)	Ubiquitination	-	43.78	21890473
442	Ubiquitination	PDVLKIYKSELNKHI HHHHHHHHHHHHHHC	40.71	27667366
447	Ubiquitination	IYKSELNKHIEDGMG HHHHHHHHHCCCCCC	59.59	21906983
467 (in isoform 1)	Ubiquitination	-	12.29	21890473
475 (in isoform 1)	Ubiquitination	-	35.80	21890473
481	Ubiquitination	QEIIENLKPLLPAGI HHHHHHCHHHCCCCH	45.24	29967540
491	Ubiquitination	LPAGIQDKLHTLIPC CCCCHHHHHHHHEEC	26.28	23000965
496	Ubiquitination	QDKLHTLIPCKKFDL HHHHHHHEECCCCCC	3.85	21963094
499	Ubiquitination	LHTLIPCKKFDLSYN HHHHEECCCCCCCCC	51.63	23000965
500	Ubiquitination	HTLIPCKKFDLSYNL HHHEECCCCCCCCCC	51.32	23000965
506	Ubiquitination	KKFDLSYNLNYHKLC CCCCCCCCCCHHHHC	20.59	21963094
523	Ubiquitination	FQEDIVFRFSLGWSS CCCCCHHEHHHCHHH	13.66	21963094
528 (in isoform 1)	Ubiquitination	-	7.05	21890473
542	Ubiquitination	FLGPRNAQRVLLGLS HHCCHHHHHHHHCCC	39.29	21963094
542 (in isoform 3)	Ubiquitination	-	39.29	21890473
553	Ubiquitination	LGLSEPIFQLPRSLA HCCCCCHHHCCHHHC	9.99	23000965
560	Ubiquitination	FQLPRSLASTPTAPT HHCCHHHCCCCCCCC	16.77	23503661
566	Ubiquitination	LASTPTAPTTPATPD HCCCCCCCCCCCCCC	39.00	23503661
571	Ubiquitination	TAPTTPATPDNASQE CCCCCCCCCCCCCHH	32.17	23503661
584	Ubiquitination	QEELMITLVTGLASV HHHHHHHHHHHHHHC	1.94	23503661
596 (in isoform 3)	Ubiquitination	-	17.57	-
602 (in isoform 3)	Ubiquitination	-	3.73	-
607 (in isoform 3)	Ubiquitination	-	6.85	21890473
620 (in isoform 3)	Ubiquitination	-	10.80	21890473
638	Ubiquitination	LSWTTHAKERAFKQQ HCCCHHHHHHHHHHH	39.88	22817900
643	Ubiquitination	HAKERAFKQQFVNYA HHHHHHHHHHHHHHH	42.05	21963094
649	Phosphorylation	FKQQFVNYATEKLRM HHHHHHHHHHHHHHH	15.03	25690035
651	Phosphorylation	QQFVNYATEKLRMIV HHHHHHHHHHHHHHH	24.24	25690035
653	Ubiquitination	FVNYATEKLRMIVSS HHHHHHHHHHHHHHC	36.72	21906983
670	Ubiquitination	ANCSHQVKQQIATTF CCCCHHHHHHHHHHH	29.89	21963094
681 (in isoform 1)	Ubiquitination	-	1.90	21890473
687	Phosphorylation	LCQQVDITQKQLEEE HHHHCCCCHHHHHHH	25.83	-
689	Ubiquitination	QQVDITQKQLEEEIA HHCCCCHHHHHHHHH	48.47	21963094
700	Ubiquitination	EEIARLPKEIDQLEK HHHHHCHHHHHHHHH	72.41	23000965
707	Ubiquitination	KEIDQLEKIQNNSKL HHHHHHHHHHHCHHH	59.18	23503661
713	Ubiquitination	EKIQNNSKLLRNKAV HHHHHCHHHHHHHHH	54.38	23503661
718	Ubiquitination	NSKLLRNKAVQLENE CHHHHHHHHHHHHHH	43.42	21906983
731	Ubiquitination	NELENFTKQFLPSSN HHHHHHHHHHCCCCC	34.61	29967540
746 (in isoform 1)	Ubiquitination	-		21890473
759 (in isoform 1)	Ubiquitination	-		21890473

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	PRKN	O60260	PMID:20871098
-	K	Ubiquitination	E3 ubiquitin ligase	MARCHF5	Q9NX47	PMID:20103533
-	K	Ubiquitination	E3 ubiquitin ligase	FZR1	Q9UM11	PMID:23045728
-	K	Ubiquitination	E3 ubiquitin ligase	AMFR	Q9UKV5	PMID:23427266

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MFN1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MFN1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CCNB1_HUMAN	CCNB1	physical	23253261
MARH5_HUMAN	MARCH5	physical	24722297
S2538_HUMAN	SLC25A38	physical	26813789
MFN2_HUMAN	MFN2	physical	28514442
FLNC_HUMAN	FLNC	physical	28514442
MGRN1_HUMAN	MGRN1	physical	27713096
MFN1_HUMAN	MFN1	physical	27713096

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.