MELK_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MELK_MOUSE
• UniProt AC: Q61846
• Protein Name: Maternal embryonic leucine zipper kinase
• Gene Name: Melk
• Organism: Mus musculus (Mouse).
• Sequence Length: 643
• Subcellular Localization: Cell membrane; Peripheral membrane protein.
• Protein Description: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis..
• Protein Sequence: MKDYDELLKYYELYETIGTGGFAKVKLACHVLTGEMVAIKIMDKNALGSDLPRVKTEIDALKSLRHQHICQLYHVLETKNKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQILSAVAYVHSQGYAHRDLKPENLLFDENHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMRGKYEVPKWLSPSSILLLQQMLQVDPKKRISMRNLLNHPWVMQDYSCPVEWQSKTPLTHLDEDCVTELSVHHRSSRQTMEDLISSWQYDHLTATYLLLLAKKARGKPARLQLLSFSCGTASTTPKSKNLSLEDMSTSDDNCVAGLIDYELCEDKLLAPKTPQVTKHLAESNHAASKSPAPGVRRAVANKLMDKENVCTPKSSVKNEEQFVFSEPKIPVSKNQYKREIPASPTRFPTPAKARAQCLREAPVRTPGNSAGADTLTTGVISPERRCRSMDVDLNQAHMEDTPKKKGTNVFGSLERGLDKVLTALTRNKKKGSARDGPRKRKLHYNVTTTRLVNPDQLLSEIMAILPKKNVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQRPDVVGIRRQRLKGDAWVYKRLVEDILSGCKM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MKDYDELLKYY ----CCCHHHHHHHH	13.05	22499769
49	Phosphorylation	MDKNALGSDLPRVKT CCCCCCCCCCCHHHH	36.50	20139300
56	Phosphorylation	SDLPRVKTEIDALKS CCCCHHHHHHHHHHH	35.00	-
73	Phosphorylation	HQHICQLYHVLETKN HHHHHHHHHHHHHCC	2.40	25168779
78	Phosphorylation	QLYHVLETKNKIFMV HHHHHHHHCCEEEEE	35.44	25168779
118	Phosphorylation	VVFRQILSAVAYVHS HHHHHHHHHHHHHHH	23.48	-
122	Phosphorylation	QILSAVAYVHSQGYA HHHHHHHHHHHCCCC	7.66	-
125	Phosphorylation	SAVAYVHSQGYAHRD HHHHHHHHCCCCCCC	18.43	-
128	Phosphorylation	AYVHSQGYAHRDLKP HHHHHCCCCCCCCCH	7.50	-
163	Phosphorylation	KPKGNKDYHLQTCCG CCCCCCCCCHHHHHH	13.16	-
167	Phosphorylation	NKDYHLQTCCGSLAY CCCCCHHHHHHHHHH	19.00	-
171	Phosphorylation	HLQTCCGSLAYAAPE CHHHHHHHHHHHCHH	8.91	-
253	Phosphorylation	VDPKKRISMRNLLNH CCCCCCCCHHHHHCC	18.74	-
336	Phosphorylation	PARLQLLSFSCGTAS CCEEEEEEEECCCCC	24.57	27149854
338	Phosphorylation	RLQLLSFSCGTASTT EEEEEEEECCCCCCC	14.30	27149854
341	Phosphorylation	LLSFSCGTASTTPKS EEEEECCCCCCCCCC	23.05	27149854
343	Phosphorylation	SFSCGTASTTPKSKN EEECCCCCCCCCCCC	32.43	27149854
344	Phosphorylation	FSCGTASTTPKSKNL EECCCCCCCCCCCCC	44.66	27149854
345	Phosphorylation	SCGTASTTPKSKNLS ECCCCCCCCCCCCCC	26.65	27149854
348	Phosphorylation	TASTTPKSKNLSLED CCCCCCCCCCCCHHH	28.16	24759943
352	Phosphorylation	TPKSKNLSLEDMSTS CCCCCCCCHHHCCCC	38.82	27087446
357	Phosphorylation	NLSLEDMSTSDDNCV CCCHHHCCCCCCCCH	36.76	25619855
358	Phosphorylation	LSLEDMSTSDDNCVA CCHHHCCCCCCCCHH	29.54	25619855
359	Phosphorylation	SLEDMSTSDDNCVAG CHHHCCCCCCCCHHH	35.34	25619855
370	Phosphorylation	CVAGLIDYELCEDKL CHHHHHCHHHHCCHH	12.10	-
382	Phosphorylation	DKLLAPKTPQVTKHL CHHCCCCCHHHHHHH	20.21	27600695
392	Phosphorylation	VTKHLAESNHAASKS HHHHHHHHCCCCCCC	28.45	28066266
397	Phosphorylation	AESNHAASKSPAPGV HHHCCCCCCCCCHHH	34.15	22345495
399	Phosphorylation	SNHAASKSPAPGVRR HCCCCCCCCCHHHHH	24.65	26824392
423	Phosphorylation	ENVCTPKSSVKNEEQ CCCCCCHHHCCCHHH	41.38	-
424	Phosphorylation	NVCTPKSSVKNEEQF CCCCCHHHCCCHHHE	43.14	19367708
434	Phosphorylation	NEEQFVFSEPKIPVS CHHHEECCCCCCCCC	47.92	26643407
452	Phosphorylation	YKREIPASPTRFPTP HCCCCCCCCCCCCCH	22.81	26824392
454	Phosphorylation	REIPASPTRFPTPAK CCCCCCCCCCCCHHH	42.31	24453211
458	Phosphorylation	ASPTRFPTPAKARAQ CCCCCCCCHHHHHHH	32.92	26745281
474	Phosphorylation	LREAPVRTPGNSAGA HHHCCCCCCCCCCCC	35.46	25619855
478	Phosphorylation	PVRTPGNSAGADTLT CCCCCCCCCCCCCCC	33.71	25619855
483	Phosphorylation	GNSAGADTLTTGVIS CCCCCCCCCCCCCCC	26.01	25619855
485	Phosphorylation	SAGADTLTTGVISPE CCCCCCCCCCCCCHH	24.48	25619855
486	Phosphorylation	AGADTLTTGVISPER CCCCCCCCCCCCHHH	32.34	25619855
490	Phosphorylation	TLTTGVISPERRCRS CCCCCCCCHHHHHCC	20.50	25338131
497	Phosphorylation	SPERRCRSMDVDLNQ CHHHHHCCCCCCCHH	23.98	26824392
510	Phosphorylation	NQAHMEDTPKKKGTN HHHHHCCCCCCCCCC	24.57	28066266
516	Phosphorylation	DTPKKKGTNVFGSLE CCCCCCCCCHHHHHH	36.77	25619855
521	Phosphorylation	KGTNVFGSLERGLDK CCCCHHHHHHHHHHH	18.65	27087446
531	Phosphorylation	RGLDKVLTALTRNKK HHHHHHHHHHHHCCC	23.70	-
553	Phosphorylation	PRKRKLHYNVTTTRL CCCCCEECEEEECCC	23.05	29514104
556	Phosphorylation	RKLHYNVTTTRLVNP CCEECEEEECCCCCH	20.86	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MELK_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
167	T	Phosphorylation	Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation.	-
171	S	Phosphorylation	Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MELK_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ZN622_HUMAN	ZNF622	physical	11802789
ZN622_HUMAN	ZNF622	physical	23747528
THIO_HUMAN	TXN	physical	23747528
THIO_MOUSE	Txn1	physical	23747528
UBC_HUMAN	UBC	physical	23747528
MDM2_HUMAN	MDM2	physical	23747528
SMAD3_HUMAN	SMAD3	physical	23747528
SMAD7_HUMAN	SMAD7	physical	23747528
M3K5_HUMAN	MAP3K5	physical	23747528
P53_HUMAN	TP53	physical	23747528

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.