MBD4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MBD4_HUMAN
• UniProt AC: O95243
• Protein Name: Methyl-CpG-binding domain protein 4
• Gene Name: MBD4
• Organism: Homo sapiens (Human).
• Sequence Length: 580
• Subcellular Localization: Nucleus.
• Protein Description: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein..
• Protein Sequence: MGTTGLESLSLGDRGAAPTVTSSERLVPDPPNDLRKEDVAMELERVGEDEEQMMIKRSSECNPLLQEPIASAQFGATAGTECRKSVPCGWERVVKQRLFGKTAGRFDVYFISPQGLKFRSKSSLANYLHKNGETSLKPEDFDFTVLSKRGIKSRYKDCSMAALTSHLQNQSNNSNWNLRTRSKCKKDVFMPPSSSSELQESRGLSNFTSTHLLLKEDEGVDDVNFRKVRKPKGKVTILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETLSVTSEENSLVKKKERSLSSGSNFCSEQKTSGIINKFCSAKDSEHNEKYEDTFLESEEIGTKVEVVERKEHLHTDILKRGSEMDNNCSPTRKDFTGEKIFQEDTIPRTQIERRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWKFLEKYPSAEVARTADWRDVSELLKPLGLYDLRAKTIVKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MGTTGLESLS -----CCCCCCCCCC	19.18	24719451
8	Phosphorylation	MGTTGLESLSLGDRG CCCCCCCCCCCCCCC	28.44	24719451
10	Phosphorylation	TTGLESLSLGDRGAA CCCCCCCCCCCCCCC	39.32	24719451
22	Phosphorylation	GAAPTVTSSERLVPD CCCCCCCCCCCCCCC	26.35	25627689
36	Ubiquitination	DPPNDLRKEDVAMEL CCCCCCCHHHHHHHH	66.61	29967540
41	Sulfoxidation	LRKEDVAMELERVGE CCHHHHHHHHHHHCC	6.41	21406390
56	Ubiquitination	DEEQMMIKRSSECNP CHHHHHHHHCCCCCH	28.47	-
56	Ubiquitination	DEEQMMIKRSSECNP CHHHHHHHHCCCCCH	28.47	-
85	Ubiquitination	AGTECRKSVPCGWER CCCCHHCCCCCCHHH	16.37	22817900
89	Ubiquitination	CRKSVPCGWERVVKQ HHCCCCCCHHHHHHH	24.18	21890473
109	Phosphorylation	TAGRFDVYFISPQGL CCCCEEEEEECCCCC	9.31	29396449
112	Phosphorylation	RFDVYFISPQGLKFR CEEEEEECCCCCCCC	10.98	29396449
117	Ubiquitination	FISPQGLKFRSKSSL EECCCCCCCCCHHHH	45.62	-
117	Ubiquitination	FISPQGLKFRSKSSL EECCCCCCCCCHHHH	45.62	-
122	Phosphorylation	GLKFRSKSSLANYLH CCCCCCHHHHHHHHH	31.65	28555341
137	Ubiquitination	KNGETSLKPEDFDFT HCCCCCCCHHCCCEE	46.24	29967540
148	Ubiquitination	FDFTVLSKRGIKSRY CCEEEEECCCCCHHH	50.94	29967540
162	Ubiquitination	YKDCSMAALTSHLQN HHHHHHHHHHHHHHH	11.61	22817900
165	Phosphorylation	CSMAALTSHLQNQSN HHHHHHHHHHHHCCC	24.05	19829383
166	Ubiquitination	SMAALTSHLQNQSNN HHHHHHHHHHHCCCC	26.63	21890473
166	Ubiquitination	SMAALTSHLQNQSNN HHHHHHHHHHHCCCC	26.63	21890473
186	Ubiquitination	RTRSKCKKDVFMPPS CCHHCCCCCCCCCCC	69.47	29967540
200	Ubiquitination	SSSSELQESRGLSNF CCHHHHHHHHCCCCC	55.84	29967540
208	Ubiquitination	SRGLSNFTSTHLLLK HHCCCCCCCHHEEEE	36.65	29967540
211	Ubiquitination	LSNFTSTHLLLKEDE CCCCCCHHEEEECCC	17.78	29967540
215	Ubiquitination	TSTHLLLKEDEGVDD CCHHEEEECCCCCCC	64.19	29967540
227	Ubiquitination	VDDVNFRKVRKPKGK CCCCCCEECCCCCCC	42.10	29967540
230	Ubiquitination	VNFRKVRKPKGKVTI CCCEECCCCCCCEEE	54.81	29967540
232	Acetylation	FRKVRKPKGKVTILK CEECCCCCCCEEEEE	74.52	7378483
234	Acetylation	KVRKPKGKVTILKGI ECCCCCCCEEEEECC	42.19	7378493
239	Sumoylation	KGKVTILKGIPIKKT CCCEEEEECCCCCCC	51.44	-
239	Acetylation	KGKVTILKGIPIKKT CCCEEEEECCCCCCC	51.44	25953088
239	Sumoylation	KGKVTILKGIPIKKT CCCEEEEECCCCCCC	51.44	-
239	Ubiquitination	KGKVTILKGIPIKKT CCCEEEEECCCCCCC	51.44	29967540
244	Ubiquitination	ILKGIPIKKTKKGCR EEECCCCCCCCCCCC	49.38	29967540
253	Phosphorylation	TKKGCRKSCSGFVQS CCCCCCHHCCCCCCC	8.31	21815630
255	Phosphorylation	KGCRKSCSGFVQSDS CCCCHHCCCCCCCCC	42.21	21815630
258	Ubiquitination	RKSCSGFVQSDSKRE CHHCCCCCCCCCHHH	6.42	29967540
262	Phosphorylation	SGFVQSDSKRESVCN CCCCCCCCHHHHHCC	38.57	19829383
266	Phosphorylation	QSDSKRESVCNKADA CCCCHHHHHCCHHHC	35.68	29978859
270	Sumoylation	KRESVCNKADAESEP HHHHHCCHHHCCCCC	42.38	-
270	Sumoylation	KRESVCNKADAESEP HHHHHCCHHHCCCCC	42.38	-
281	Acetylation	ESEPVAQKSQLDRTV CCCCCCCHHHCCCEE	30.36	25953088
281	Ubiquitination	ESEPVAQKSQLDRTV CCCCCCCHHHCCCEE	30.36	29967540
304	Phosphorylation	GETLSVTSEENSLVK CCEEEECHHHCHHHH	40.53	-
308	Phosphorylation	SVTSEENSLVKKKER EECHHHCHHHHHHHH	37.51	-
316	Phosphorylation	LVKKKERSLSSGSNF HHHHHHHHCCCCCCC	33.42	25159151
318	Phosphorylation	KKKERSLSSGSNFCS HHHHHHCCCCCCCCC	33.62	23401153
319	Phosphorylation	KKERSLSSGSNFCSE HHHHHCCCCCCCCCC	52.08	25159151
321	Phosphorylation	ERSLSSGSNFCSEQK HHHCCCCCCCCCCHH	28.78	25159151
325	Phosphorylation	SSGSNFCSEQKTSGI CCCCCCCCCHHCHHH	38.64	28102081
329	Phosphorylation	NFCSEQKTSGIINKF CCCCCHHCHHHHHHH	31.70	29978859
330	Phosphorylation	FCSEQKTSGIINKFC CCCCHHCHHHHHHHH	34.72	29978859
335	Acetylation	KTSGIINKFCSAKDS HCHHHHHHHHCCCCC	37.02	25953088
335	Ubiquitination	KTSGIINKFCSAKDS HCHHHHHHHHCCCCC	37.02	29967540
348	Phosphorylation	DSEHNEKYEDTFLES CCCCCHHHHHCCHHH	16.92	27642862
368	Sumoylation	KVEVVERKEHLHTDI EEEEEECHHHHCHHH	35.66	-
368	Sumoylation	KVEVVERKEHLHTDI EEEEEECHHHHCHHH	35.66	-
368	Ubiquitination	KVEVVERKEHLHTDI EEEEEECHHHHCHHH	35.66	29967540
377	Ubiquitination	HLHTDILKRGSEMDN HHCHHHHHHCHHCCC	55.86	29967540
380	Phosphorylation	TDILKRGSEMDNNCS HHHHHHCHHCCCCCC	33.51	27794612
387	Phosphorylation	SEMDNNCSPTRKDFT HHCCCCCCCCCCCCC	31.28	23401153
387 (in isoform 2)	Phosphorylation	-	31.28	29083192
389	Phosphorylation	MDNNCSPTRKDFTGE CCCCCCCCCCCCCCC	33.77	29255136
389 (in isoform 2)	Phosphorylation	-	33.77	29083192
391	Ubiquitination	NNCSPTRKDFTGEKI CCCCCCCCCCCCCCC	60.72	29967540
394	Phosphorylation	SPTRKDFTGEKIFQE CCCCCCCCCCCCCCC	55.34	23532336
394 (in isoform 2)	Phosphorylation	-	55.34	28634120
397	Ubiquitination	RKDFTGEKIFQEDTI CCCCCCCCCCCCCCC	50.60	-
397	Ubiquitination	RKDFTGEKIFQEDTI CCCCCCCCCCCCCCC	50.60	29967540
397 (in isoform 2)	Phosphorylation	-	50.60	24825855
414	Phosphorylation	TQIERRKTSLYFSSK HHHHHHHCCEEECHH	23.35	28450419
415	Phosphorylation	QIERRKTSLYFSSKY HHHHHHCCEEECHHC	24.90	28450419
417	Phosphorylation	ERRKTSLYFSSKYNK HHHHCCEEECHHCCH	10.94	29496907
419	Phosphorylation	RKTSLYFSSKYNKEA HHCCEEECHHCCHHH	16.62	29978859
421	Acetylation	TSLYFSSKYNKEALS CCEEECHHCCHHHCC	52.20	26051181
428	Phosphorylation	KYNKEALSPPRRKAF HCCHHHCCCCCHHHH	39.65	23401153
439	Phosphorylation	RKAFKKWTPPRSPFN HHHHHHCCCCCCCCC	32.09	21857030
463	Phosphorylation	PWKLLIATIFLNRTS HHHHHHHHHHHHCCC	12.79	20860994
474	Ubiquitination	NRTSGKMAIPVLWKF HCCCCCCHHHHHHHH	13.35	22817900
478	Ubiquitination	GKMAIPVLWKFLEKY CCCHHHHHHHHHHHC	3.27	21890473
478 (in isoform 2)	Ubiquitination	-	3.27	21890473
480	Ubiquitination	MAIPVLWKFLEKYPS CHHHHHHHHHHHCCC	35.59	22817900
484	Ubiquitination	VLWKFLEKYPSAEVA HHHHHHHHCCCHHHH	65.69	21890473
484	Ubiquitination	VLWKFLEKYPSAEVA HHHHHHHHCCCHHHH	65.69	-
484	Ubiquitination	VLWKFLEKYPSAEVA HHHHHHHHCCCHHHH	65.69	22817900
484 (in isoform 1)	Ubiquitination	-	65.69	21890473
484 (in isoform 3)	Ubiquitination	-	65.69	21890473
498	Ubiquitination	ARTADWRDVSELLKP HHCCCHHCHHHHHHH	44.00	29967540
504	Ubiquitination	RDVSELLKPLGLYDL HCHHHHHHHHCCCCC	51.31	-
504	Ubiquitination	RDVSELLKPLGLYDL HCHHHHHHHHCCCCC	51.31	29967540
512	Ubiquitination	PLGLYDLRAKTIVKF HHCCCCCCCCEEEEE	31.01	29967540
518	Ubiquitination	LRAKTIVKFSDEYLT CCCCEEEEECHHHHH	34.99	29967540
520	Ubiquitination	AKTIVKFSDEYLTKQ CCEEEEECHHHHHHC	24.66	29967540
523	Ubiquitination	IVKFSDEYLTKQWKY EEEECHHHHHHCCCC	25.35	29967540
526	Ubiquitination	FSDEYLTKQWKYPIE ECHHHHHHCCCCCEE	52.12	-
526	Ubiquitination	FSDEYLTKQWKYPIE ECHHHHHHCCCCCEE	52.12	29967540
529	Ubiquitination	EYLTKQWKYPIELHG HHHHHCCCCCEEECC	38.36	29967540
554	Ubiquitination	IFCVNEWKQVHPEDH EEEECCCEECCCCCC	35.94	-
556	Ubiquitination	CVNEWKQVHPEDHKL EECCCEECCCCCCCH	7.84	29967540
562	Ubiquitination	QVHPEDHKLNKYHDW ECCCCCCCHHHHHHH	67.11	29967540
570	Ubiquitination	LNKYHDWLWENHEKL HHHHHHHHHHCCHHH	5.25	29967540
576	Ubiquitination	WLWENHEKLSLS--- HHHHCCHHHCCC---	35.51	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
165	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
262	S	Phosphorylation	Kinase	PRKCA	P17252	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MBD4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MBD4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FADD_HUMAN	FADD	physical	12702765
SIN3A_HUMAN	SIN3A	physical	15899845
HDAC1_HUMAN	HDAC1	physical	15899845
TRI27_MOUSE	Trim27	physical	17049487
TRI27_HUMAN	TRIM27	physical	17049487
CDCA2_HUMAN	CDCA2	physical	21900206
HD_HUMAN	HTT	physical	23275563
KDM1A_HUMAN	KDM1A	physical	23455924
SUV91_HUMAN	SUV39H1	physical	23455924
UHRF1_HUMAN	UHRF1	physical	25358258
UBP7_HUMAN	USP7	physical	25358258
MLH1_HUMAN	MLH1	physical	25358258
UHRF1_MOUSE	Uhrf1	physical	25358258

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.