LOXE3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: LOXE3_HUMAN
• UniProt AC: Q9BYJ1
• Protein Name: Hydroperoxide isomerase ALOXE3
• Gene Name: ALOXE3
• Organism: Homo sapiens (Human).
• Sequence Length: 711
• Subcellular Localization: Cytoplasm .
• Protein Description: Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia..
• Protein Sequence: MAVYRLCVTTGPYLRAGTLDNISVTLVGTCGESPKQRLDRMGRDFAPGSVQKYKVRCTAELGELLLLRVHKERYAFFRKDSWYCSRICVTEPDGSVSHFPCYQWIEGYCTVELRPGTARTICQDSLPLLLDHRTRELRARQECYRWKIYAPGFPCMVDVNSFQEMESDKKFALTKTTTCVDQGDSSGNRYLPGFPMKIDIPSLMYMEPNVRYSATKTISLLFNAIPASLGMKLRGLLDRKGSWKKLDDMQNIFWCHKTFTTKYVTEHWCEDHFFGYQYLNGVNPVMLHCISSLPSKLPVTNDMVAPLLGQDTCLQTELERGNIFLADYWILAEAPTHCLNGRQQYVAAPLCLLWLSPQGALVPLAIQLSQTPGPDSPIFLPTDSEWDWLLAKTWVRNSEFLVHENNTHFLCTHLLCEAFAMATLRQLPLCHPIYKLLLPHTRYTLQVNTIARATLLNPEGLVDQVTSIGRQGLIYLMSTGLAHFTYTNFCLPDSLRARGVLAIPNYHYRDDGLKIWAAIESFVSEIVGYYYPSDASVQQDSELQAWTGEIFAQAFLGRESSGFPSRLCTPGEMVKFLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGTTTLKTYLDTLPEVNISCNNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRRSIAAFQSRLAQISRDIQERNQGLALPYTYLDPPLIENSVSI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
44 (in isoform 2)	Phosphorylation	-	38.03	-
44	Phosphorylation	RLDRMGRDFAPGSVQ HHHHCCCCCCCCCCC	38.03	-
52	Ubiquitination	FAPGSVQKYKVRCTA CCCCCCCCEEEEEEE	44.90	29967540
117	Phosphorylation	TVELRPGTARTICQD EEEECCCCHHHHHHC	18.69	10394743
134	Phosphorylation	PLLLDHRTRELRARQ HHHHCHHHHHHHHHH	25.69	10394753
169	Ubiquitination	FQEMESDKKFALTKT HHHHHCCCCEEEEEE	60.10	23000965
170	Ubiquitination	QEMESDKKFALTKTT HHHHCCCCEEEEEEE	40.64	23000965
175	Ubiquitination	DKKFALTKTTTCVDQ CCCEEEEEEEEEEEC	44.73	23000965
176	Phosphorylation	KKFALTKTTTCVDQG CCEEEEEEEEEEECC	23.31	50564823
184	Ubiquitination	TTCVDQGDSSGNRYL EEEEECCCCCCCCCC	33.72	29967540
295	Phosphorylation	HCISSLPSKLPVTND HHHHCCCCCCCCCCC	52.71	24719451
300	Phosphorylation	LPSKLPVTNDMVAPL CCCCCCCCCCCHHHH	24.46	24719451
301	Ubiquitination	PSKLPVTNDMVAPLL CCCCCCCCCCHHHHH	35.30	23000965
302	Ubiquitination	SKLPVTNDMVAPLLG CCCCCCCCCHHHHHC	24.46	23000965
307	Ubiquitination	TNDMVAPLLGQDTCL CCCCHHHHHCCCCHH	6.39	23000965
316	Phosphorylation	GQDTCLQTELERGNI CCCCHHHHHHHHCCE	29.59	24719451
407	Phosphorylation	FLVHENNTHFLCTHL EEEECCCCHHHHHHH	26.11	22468782
423	Phosphorylation	CEAFAMATLRQLPLC HHHHHHHHHHCCCCC	14.91	22468782
434	Phosphorylation	LPLCHPIYKLLLPHT CCCCCHHHHHHCCCC	10.47	46106903
578	Phosphorylation	GEMVKFLTAIIFNCS HHHHHHHHHHHHHCC	20.95	27251275
593	Phosphorylation	AQHAAVNSGQHDFGA HHCHHHHCCCCCCCC	32.98	27251275
607	Phosphorylation	AWMPNAPSSMRQPPP CCCCCCCCCCCCCCC	34.06	27251275
608	Phosphorylation	WMPNAPSSMRQPPPQ CCCCCCCCCCCCCCC	19.54	27251275
616	Phosphorylation	MRQPPPQTKGTTTLK CCCCCCCCCCCCCHH	36.90	27251275
617	Ubiquitination	RQPPPQTKGTTTLKT CCCCCCCCCCCCHHH	49.35	27667366
677	Phosphorylation	RSIAAFQSRLAQISR HHHHHHHHHHHHHHH	23.86	46106909
739	Phosphorylation	----------------------------------- -----------------------------------		27251275
749	Ubiquitination	--------------------------------------------- ---------------------------------------------		27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of LOXE3_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of LOXE3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of LOXE3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NBEA_HUMAN	NBEA	physical	28514442
ZFHX4_HUMAN	ZFHX4	physical	28514442
KTU_HUMAN	DNAAF2	physical	28514442
SRC_HUMAN	SRC	physical	28514442
HSP7C_HUMAN	HSPA8	physical	28514442
EDEM3_HUMAN	EDEM3	physical	28514442
HOOK1_HUMAN	HOOK1	physical	28514442
HERC4_HUMAN	HERC4	physical	28514442
CH60_HUMAN	HSPD1	physical	28514442

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.