IMPCT_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IMPCT_HUMAN
• UniProt AC: Q9P2X3
• Protein Name: Protein IMPACT
• Gene Name: IMPACT
• Organism: Homo sapiens (Human).
• Sequence Length: 320
• Subcellular Localization: Cytoplasm .
• Protein Description: Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth..
• Protein Sequence: MAEGDAGSDQRQNEEIEAMAAIYGEEWCVIDDCAKIFCIRISDDIDDPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSLEEIYIQNIGESILYLWVEKIRDVLIQKSQMTEPGPDVKKKTEEEDVECEDDLILACQPESSLKALDFDISETRTEVEVEELPPIDHGIPITDRRSTFQAHLAPVVCPKQVKMVLSKLYENKKIASATHNIYAYRIYCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNYTNSPEESSKALGKNKKVRKDKKRNEH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
51	Phosphorylation	DIDDPKWTLCLQVML CCCCCCEEEEEEEEC	17.45	24043423
62	Phosphorylation	QVMLPNEYPGTAPPI EEECCCCCCCCCCCE	17.38	24043423
65	Phosphorylation	LPNEYPGTAPPIYQL CCCCCCCCCCCEEEC	31.33	24043423
70	Phosphorylation	PGTAPPIYQLNAPWL CCCCCCEEECCCHHH	16.84	24043423
127	Ubiquitination	TEPGPDVKKKTEEED CCCCCCCCCCCCCCC	56.80	-
127 (in isoform 1)	Ubiquitination	-	56.80	21906983
150	Phosphorylation	LACQPESSLKALDFD EEECCHHHHHHCCCC	32.02	27251275
159	Phosphorylation	KALDFDISETRTEVE HHCCCCCCCCCEEEE	34.46	21815630
161	Phosphorylation	LDFDISETRTEVEVE CCCCCCCCCEEEEEE	35.78	21815630
200	Ubiquitination	VVCPKQVKMVLSKLY CCCHHHHHHHHHHHH	22.31	-
207	Phosphorylation	KMVLSKLYENKKIAS HHHHHHHHCCCCHHH	22.61	-
211	Ubiquitination	SKLYENKKIASATHN HHHHCCCCHHHCCCC	55.90	-
229	Ubiquitination	YRIYCEDKQTFLQDC EEEEECCHHEEECCC	29.01	-
229	Malonylation	YRIYCEDKQTFLQDC EEEEECCHHEEECCC	29.01	26320211
229	Acetylation	YRIYCEDKQTFLQDC EEEEECCHHEEECCC	29.01	26822725
292	Ubiquitination	ARNILVEKNYTNSPE HHHHHEECCCCCCHH	48.01	-
295	Phosphorylation	ILVEKNYTNSPEESS HHEECCCCCCHHHHH	38.32	29396449
297	Phosphorylation	VEKNYTNSPEESSKA EECCCCCCHHHHHHH	26.37	23663014
301	Phosphorylation	YTNSPEESSKALGKN CCCCHHHHHHHHCCC	35.16	23663014
302	Phosphorylation	TNSPEESSKALGKNK CCCHHHHHHHHCCCC	25.81	29396449

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF1	Q9HCE7	PMID:20804422

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of IMPCT_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IMPCT_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GCN1_YEAST	GCN1	physical	22404850
ACT_YEAST	ACT1	physical	22404850
RL39_YEAST	RPL39	physical	22404850
RS22A_YEAST	RPS22A	physical	22404850
RS22B_YEAST	RPS22B	physical	22404850
CRHBP_HUMAN	CRHBP	physical	28514442

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.