HIW_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HIW_DROME
• UniProt AC: Q9NB71
• Protein Name: E3 ubiquitin-protein ligase highwire {ECO:0000303|PubMed:10839352}
• Gene Name: hiw {ECO:0000303|PubMed:10839352, ECO:0000312|FlyBase:FBgn0030600}
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 5233
• Subcellular Localization: Cell junction, synapse . Cell projection, axon .
• Protein Description: Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues (By similarity). Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates (By similarity). Required in the presynaptic motoneuron to down-regulate the levels of wnd and restrain synaptic terminal growth at the neuromuscular junction (NMJ) together with Rae1 and Fsn. [PubMed: 10839352]
• Protein Sequence: MVGLLNPLKYGDHFYELYTNSTRRKMQHDRKVLTKRKSRKDKSAAAMAAAAAAAVLEGNAGAGGLVNPLDPPLVKEQLMILPSFPVAHIELDPNASKFAVFSAIRSTVLEAETRYSLFQEGGAPSGQSGSNQKKWSNNVNSACCMMCAHSCMISNLLDCSCHAHMAMHGAVGPGNAGPGGPVGVLVDGRIPAPVPVVVGGPGLPAEKRPRRDSANSDADSDTEEPTEREPVYATVPLIVGAGLRSLFELIADARHVHPLLCTKALKALLDVIQGQQPESFKLEPEELINPLYDLLLDLATMPAALNSATGAEANWSAMACAALLGLCIARGDTGKMLKAIAAMLMTPRQLSAQIVQLPVVLATLQHTVISAALNKPTRPDFHSHGVPHNSLIDEFPVKLPPLSTGAPITSPAMACDGVFVYLLYGGTLLKIGTGFGGSYKGHIYAQNEDFSHERSAWLGYSGGQLYFRRTCRRSAGDQLQMVGLDTLAIKAMSPLSMLHMREGLNYVLFTDDDSLHAICSNRDDTLVVKKLNLYHNSYNIDPPPFELPLQLARKKFRTLGYAAFEDELLNQYQIQRIQSAHNSFEPKLPAPCRDADVDVMGMACGKEFGLVRASNGRVYYYGKSAALGLKCVGRTPTLKLTELVISKAANIVHVAVGHDGIHALLVNDDGTVFFAGTARRGEDGDSSKNRRQPKAVKPKKMTKIDGHVVVHAACNNGTSAFVTKTGKLIMYGKDTAHCDAMGFVSELLEQHVTKVALGKAHCVALNAKGQLFSFGLNNKGQCGRIFNKLQPVKDVPPFASSSTAAACFASLLPLDKRLKLDFSTLCDYDDHNLVQGQCRVCVICRECTGYNVSCVSALNVPLDQRLAGSICPCGHGDAGCAKCGLCAACIALQDSDEAKTELKPPPSDVQQRQQRSKTLIMRRKERKGELETGAAGGGAATPTDLDKDPPRVAPLAPQLLQLTSSSPVVQVACGLHHTVVLTLAGEVYTFGSNQYGQLGSGDLQPVSGPVRVQVPGAISQVAAGSNHTVLLTSKGMVYTFGNYQKGQLGRLPSDYGLKPPPQDDDSPVGAGSDGGAGGSPTVAPVGMPGPERSQSPANVQPSGSKEMPPLLPVLTQRQKFLWNCSPGAVFGLGPCYGKKVTWIGANGDQTFIKIDESLITAQMLPKMHVVANKKTILLIPSIPLSFHTLSINRRDGSCTAHYRGQTNFVKLMQAQPEQQQPEINSNLDVAVTPVADSPAHASTSSLLAALTGTATAGVPINEQMSRSMHEARNQIFEEQLPEVGSSAAAAAVAAPGTPVSAGSVPRSRRGGKQGTSSPEPIPSPPQLAFTMDPTYNVLWVFDGAARKLRCHNVVASDINDSDANAATYRSLLSPELSLPDRVDSRVARSQASLNLLACLDILTSAQDNIPGCFEQPLLKQTQQTAETQAGEFQVVNRFDNFGGGWGYSGHSVEAIRFSADTDIVICGFGMFGGRGEYSCKLKLFDLGGDGGGYEKEGILISETKEVPYECGARSKHHILLPKPVSAVAGRWYLVWARIAGPSSDCGSCGQASVTTEDQVVFSFKSSKKANNGTDVNSGQIPAILYRLVTQDCKQTPAQMDADPVQRISRAFANSVSRECFESLVVLLSWSWDCFKLQLREERDRSRPLQLQQSLQYLGYVIKSCLRLLRKYTIEIYPQRNSSTSVATGGGSNAAHGSGVVTTAKSVQSKPNKDKNTPRVVGNAGVMAKYFGDPSTSVAPAMISSASSGGAPSTSASAAVAPGSGTPVTRKTNMENIQLAECIGNVRALLIGIFCDDIFKDIATDEGYELSLEILDECHLSFVACFDAFYPTSSLKWNCLCDLLAQMDRGALHSRLLSAILAGLCSPSVKLRATFSLLSAAGNERQSIISPSDNSGLPMLSSTDAHPYPVLVEQMIYRTQQEKSDFLSNSWTFKDVLVRLLDIIASPIRSRIEAIYSRSLGSLGYPGGKDCVNQGLIDNCCHLLARVLAEIVYQTAMGEYDKLFMPPRTLHSTGARFARCDVSRTWNTGNFGPDAIAFAVDRPGVAIAGAMVYSGSGSYDYQLELLYDNTADLQPQHKWETLESVSGSYDQDAVHNDLAEIKFDHPVHIKENARYALRLCSQGARTCSGDAGMPAVRGPCGAQFHFYACDLSFNGTTPARGQLPCILYYSTPMKQDGHSASGRTGDGSNVATHLEDRIMLLGPHEVSTRDTALQIAADITKKCTELLILARNAMAASCSPSDNSSNHTQTIDSEHNITPIEEHMDINWANNSRTAALPTAIDPQLSTARDLGKRIESFSKGLMETLKFDKRSTNPFEMEIEIGATEVEESADLRNGQSQSVSQSQSQSQSVPINGNERTADFEFAEQSAQQSMPQHLHSDSEEAPLEVAGMAAGGGVSVADGSGGVAGVGSQAAAVQLLEVFNLAASNMFHTLLPLVYAHIANLACSDPKSSVQILGLIKEILPHIAALNQLHVSKDQRQPEPAIFATQTSGSGNSNSSSTTSNHYCVVESDHPYKSASISSYRVEFPPCVQWLTIEFDPQCGTAQLEDYLLLSIPMRPASQAPPVPHVDDYLEQADNNVNGAGDRRRNTGGGIAGSGAAPNTHQRSASVQLTMASCCRSPGCGNAPGSAAAPSSMPLRSQDPNDREWIVVKKFNTASTWLHNVLILPGNCVEFSLETASLYAQDPHNNRYGFKCLVVGYDNPTSINASNSCLIRLEQELAYLGGMCSANLMKKELNLPDDKDVEDMSGIEETINTHHTLLSKGFALSEPQLTVHQALESYLPIGSQSNERQFLKDFISGAPGSSGARLAAWLQPESRLDPNKCELNTITEPLRYGWPSQVTVTIRDQYGDAVLVPELKVEIKAIPTGSGPNGSATGTGTSCTSVAEVSAPGPNLWMRRASRDTWGWGGMAPPPRINYEPTVKDKMVFKAITFMKPYANYSFEELRYASPVQTRVTELLNAKDMEDGTFSVQWTPSSVGAYCLAVTIDGIPLEEVYRVDVKEGILPPPTQRNSAQRRPQAPSKLRRFQARHSSGLRIRSHPTLQSEQVGVVRVGGVISFIDEIENDDGVWLRLSTESIRQHCTMGWYPTEAWCLQFNQHLARMLLQPVTDKEVNPVRKGVGAEEDVEEQPPVTPSASGEASPEPEPDPSPVLSPAKTKPGRFLSGHQSTNPFLYPAKHADLAEREAQVQEEREKEEEQVDDEDADDREPEQEALPAVELLPAHIGSAIAGVVGGGAIKLQALQKWFKGDAVDGPQPLTPSHSPPLAGVSVRELVRAMGGQDSPRGNGNRSQQEQDPEFSLASMRRPNYSASQTAALLSTPKHTPKRSAVVASETSGLEDELSLLQITTTTTGQGEQQSELQLATTSTASSASKRNPMGPIKRAMPPSFAESIRAVFAALLWHEGVVHDAMACASFLKFHPGLPKEGATVVTRRGESGDPRLQLSREQKAQQRHSVEVANAGNYLNIRPSTLETLTKSGNCSLHNRSKYRKNLLSGGGGAINSGDDTAQKLQALPEMVSVLPPALRCLVYLWEQICSGCVQIVQSNALEQREPRLLSPGSRDLNGDADTEGKEGKNSDQASAGEKDLGRKCKRKKKDDGSWCEICELFLPMPVTYHMRIAHPGCGKSAKGKGYNSVGIFCEGWAGNCGEGGKGASSWFLMCDPCRDRYLASCRSANNINSAARQLESSAAEGNELNLFGVKSTTLIANAEVYTTMRENATFLLELCSSSSSASGAAGSLAATSSSSKRSPQQMSVVAMPVVIEHQLGNSDLKPSTSRCSRMARLSGSKFCPGVGSGAFRKSFVGGPPTAPENVWLAPESFACLECLGTAGHEDLPYEMFGLGPNSNDNGYDRPLSEISYESCEPNNYDMLSGSLAPGTTAAASVGGGNLSKFHRSYSMGQGWASLAQHNHPPPHHPQQQHHQQQQMNLQLQQHQAPPVDGQPKVVYRRRNNSTSEGDGSLLICYPSEHLRRLVPQKLLASVSVMQTASGEGTGKDHATGTLGLDQSAGQNGGGNLLLTRPAMAFITQKHELDRLRAAMRRSLRIAACRIYALQALNWLLRSVTQGVCLHDLMWWFVSSLNPTGGHQPVERGEEASEPALEHPVAYTQISGRFAHLITQSLHVFLQSVADLTLHLPLGSPLQRVAIQCFGIRFRQADHQFLHSSHVFGNISKILSKSDEQNDAMAVSTILKPDCDVEHNQVHSVATGGSSGAGARLLCYTDLAGMFEVTVSSRPAMAESLTDNSTETFWESDEEDRNKCKIIELSLTKLNYACRYLLVHIDNSRDIQNKVLNVVFYAGQSLGDTNIIKSADVDPKACSWISAKICDDSCTHFRLELHGPENTLRVRQIKLLGLPIGGAVGSDDSSDHKHQPHLRLSHASRIQQQICEAETLRVFRLITGQVFGKLISNVSSDLVPPDSAGIGPPSGGAASTSLLADSLDLREHMVGILFSRSKLSHLQKQVIVHIVHAIRKEAQRAKEDWELANLAHVLKQSPQQQTAPALAASASCESTPERSRAPDTYCFEMLSMVLALSGSVVGRSYLSQQHGLLRDLLGLLHTGSDRVQRQVTALLRRILPEITPESFAELLGVQRLPPADYSIAHQSASDFDMSRLGLLDIFLAVIAKSLQLQVKVKTTVASTGPSGSGGVSGSSSGNGGAVLKAGQQEKTPAFVRLWSSLDLSVQQLRSRPPTGEPGTTDPFQFDALPPRKESKRNLNQRWFLNGVISTKQAESIISLIRDLASGKLSEKWSQITKAAIAESVLNLTRLEEIYRSPEHCTKTSTLWLALASLCVLERDHVEKLSSGQWSKLCDTRPLCSNHDDGETAAIIQCETCGSLCGDCDRFLHLNRKTRSHKRTVCKEEEEAIRVELHESCGRTKLFWLLALADSKTLKAMVEFRDGSHTIISGPQEAVGRCRFCGLTGNSGLLEIGNVCADAQCQEYAANSCLKTKPCGHACGGVTGERKCLPCLQHVCHTRENELAEELRDPKLTQDADDMCMICFVEALSCAPSIHLECGHVFHYHCCKAVLEKRWSGPRITFGFSLCPICKADIQHPLLSDILEPINGLKQDVKRKALMRIKYEGVVKDTDSKNVNMTQLAMDRYAYYVCFKCQKAYYGGEARCDAEIGEKFDPEELVCGGCSDVARAQMCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCDTCHDDFQRLTNIPKVKLPQCPAGPKAKQLLGDECPLHVMHPPTGEEFALGCGVCRNAQTF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
213	Phosphorylation	EKRPRRDSANSDADS HHCCCCCCCCCCCCC	27.77	22817900
216	Phosphorylation	PRRDSANSDADSDTE CCCCCCCCCCCCCCC	33.61	22817900
916	Phosphorylation	VQQRQQRSKTLIMRR HHHHHHHHCHHHHHH	26.17	22668510
1253	Phosphorylation	LLAALTGTATAGVPI HHHHHHCCCCCCCCC	18.66	22817900
1255	Phosphorylation	AALTGTATAGVPINE HHHHCCCCCCCCCCH	24.63	22817900
1265	Phosphorylation	VPINEQMSRSMHEAR CCCCHHHHHHHHHHH	22.39	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of HIW_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HIW_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HIW_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MK14B_DROME	p38b	genetic	23904612
FBSP1_DROME	Fsn	genetic	17697379
FMR1_DROME	Fmr1	genetic	23764288
BECN1_DROME	Atg6	genetic	19786572
MK14A_DROME	p38a	genetic	23904612
FAF_DROME	faf	genetic	11473321
JNK_DROME	bsk	genetic	16815332
JNK_DROME	bsk	genetic	23063437
JIP1_DROME	Aplip1	genetic	23904612
FBSP1_DROME	Fsn	physical	21874015
TBB1_DROME	betaTub56D	physical	21874015

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213 AND SER-216, ANDMASS SPECTROMETRY.
PubMed: 18327897