dbPTM

GLI2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	GLI2_MOUSE
UniProt AC	Q0VGT2
Protein Name	Zinc finger protein GLI2 {ECO:0000305}
Gene Name	Gli2 {ECO:0000312\|MGI:MGI:95728}
Organism	Mus musculus (Mouse).
Sequence Length	1544
Subcellular Localization	Nucleus . Cytoplasm . Cell projection, cilium . STK36 promotes translocation to the nucleus (PubMed:10806483). In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus (PubMed:23034632).
Protein Description	Functions as transcription regulator in the hedgehog (Hh) pathway. [PubMed: 9006072 Functions as transcriptional activator]
Protein Sequence	METSAPAPALEKKEAKSGLLEDSSFPDPGKKACPLAVAAAVAAHGVPQQLLPAFHAPLPIDMRHQEGRYHYDPHSVHSVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEVVIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGLQLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALPGACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMNPGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPDDVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPSSNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPHLQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKPSHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQNAGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDTGRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDFDAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
84	Phosphorylation	HSVHGPPTLSGSPVI CCCCCCCCCCCCCCC	36.37	26060331
86	Phosphorylation	VHGPPTLSGSPVISD CCCCCCCCCCCCCCC	39.44	26060331
88	Phosphorylation	GPPTLSGSPVISDIS CCCCCCCCCCCCCCE	16.77	26060331
145	Phosphorylation	ISAARGLSPADVAHE HHHHCCCCHHHHHHH	22.36	29514104
230	Phosphorylation	LSRKRALSISPLSDA CCCCCCCCCCCCCHH	21.75	26824392
232	Phosphorylation	RKRALSISPLSDASL CCCCCCCCCCCHHHH	19.20	25338131
238	Phosphorylation	ISPLSDASLDLQRMI CCCCCHHHHHHHHHH	27.66	-
376	Phosphorylation	HKRSKVKTEAEGLRP EECCCCCCCCCCCCC	43.33	21149613
385	Phosphorylation	AEGLRPASPLGLTQE CCCCCCCCCCCCCHH	24.28	27180971
502	Phosphorylation	SRLENLKTHLRSHTG HHHHHHHHHHHHHCC	29.34	-
525	Phosphorylation	EGCNKAFSNASDRAK CCCCHHHCCHHHHHH	36.02	-
528	Phosphorylation	NKAFSNASDRAKHQN CHHHCCHHHHHHHCC	31.70	-
630	Sumoylation	CLHIKAIKTESSGLC CEEEEEEEECCCCCC	51.61	-
630	Sumoylation	CLHIKAIKTESSGLC CEEEEEEEECCCCCC	51.61	-
662	Phosphorylation	GSAPNNDSGMEMPGT CCCCCCCCCCCCCCC	42.01	-
707	Phosphorylation	LQLRKHMSTVHRFEQ HHHHHHHHHHHHHHH	27.31	19131326
708	Phosphorylation	QLRKHMSTVHRFEQL HHHHHHHHHHHHHHH	16.86	19131326
716	Sumoylation	VHRFEQLKREKLKSL HHHHHHHHHHHHHHH	59.20	-
716	Sumoylation	VHRFEQLKREKLKSL HHHHHHHHHHHHHHH	59.20	-
740	Acetylation	APHTRNTKLPPLPVN CCCCCCCCCCCCCCC	63.65	62154803
789	Phosphorylation	QLQERRDSSTSTMSS HHHHHHCCCCCCCHH	33.43	22549777
790	Phosphorylation	LQERRDSSTSTMSSA HHHHHCCCCCCCHHC	30.57	24719451
796	Phosphorylation	SSTSTMSSAYTVSRR CCCCCCHHCCEECCC	17.93	-
804	Phosphorylation	AYTVSRRSSGISPYF CCEECCCCCCCCCCC	31.63	29514104
805	Phosphorylation	YTVSRRSSGISPYFS CEECCCCCCCCCCCC	38.03	29895711
812	Phosphorylation	SGISPYFSSRRSSEA CCCCCCCCCCCCCCC	19.66	28059163
813	Phosphorylation	GISPYFSSRRSSEAS CCCCCCCCCCCCCCC	23.09	24759943
816	Phosphorylation	PYFSSRRSSEASPLG CCCCCCCCCCCCCCC	31.27	28507225
817	Phosphorylation	YFSSRRSSEASPLGG CCCCCCCCCCCCCCC	35.06	22549777
831	Phosphorylation	GLRPHNASSADSYDP CCCCCCCCCCCCCCC	31.23	-
847	Phosphorylation	STDASRRSSEASQCS CCCHHHHHCHHHHCC	31.27	25168779
848	Phosphorylation	TDASRRSSEASQCSG CCHHHHHCHHHHCCC	35.06	22549777
851	Phosphorylation	SRRSSEASQCSGGGP HHHHCHHHHCCCCCC	27.89	25168779
854	Phosphorylation	SSEASQCSGGGPGLL HCHHHHCCCCCCCCC	32.60	25168779
864	Phosphorylation	GPGLLNLTPAQQYNL CCCCCCCCHHHHHCH	18.59	25168779
885	Phosphorylation	ATGGPPPTPLPGLDR HHCCCCCCCCCCCCC	43.14	22028470
923	Phosphorylation	PLGPRRGSDGPTYSH CCCCCCCCCCCCCCC	37.27	28507225
956	Phosphorylation	NSSTRRASDPVRRPD CCCCCCCCCCCCCCC	40.41	22549777
975	Phosphorylation	PRVQRFHSTHNMNPG HCHHHCCCCCCCCCC	28.75	28507225
997	Phosphorylation	RRGLHVQSHPSVDSN CCCCCCCCCCCCCCC	35.96	-
1006	Phosphorylation	PSVDSNLTRNAYSPR CCCCCCCCCCCCCCC	26.57	22807455
1011	Phosphorylation	NLTRNAYSPRPPSIN CCCCCCCCCCCCCCC	16.42	18455992
1087	Phosphorylation	PENSKLPSPGLQGHR CCCCCCCCCCCCHHH	41.87	26824392
1118	Phosphorylation	GGCQLSYSPSSNLNK CCCCCEECCCCCCCC	18.22	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
230	S	Phosphorylation	Kinase	AKT1	P31750	PSP
385	S	Phosphorylation	Kinase	DYRK2	Q92630	PSP
385	S	Phosphorylation	Kinase	DYRK2	Q5U4C9	Uniprot
885	T	Phosphorylation	Kinase	MAPK1	P63085	GPS
997	S	Phosphorylation	Kinase	DYRK2	Q5U4C9	Uniprot
1011	S	Phosphorylation	Kinase	DYRK2	Q92630	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
740	K	Acetylation		-
Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of GLI2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FBW1A_MOUSE	Btrc	physical	16611981
FBW1B_HUMAN	FBXW11	physical	16651270
HDAC4_MOUSE	Hdac4	physical	22549777
PIAS1_MOUSE	Pias1	physical	22549777
SUMO2_MOUSE	Sumo2	physical	22549777
SPOP_MOUSE	Spop	physical	20463034
SUFU_MOUSE	Sufu	physical	20463034
TULP3_MOUSE	Tulp3	physical	19286674

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of GLI2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry."; Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.; Mol. Cell. Proteomics 8:904-912(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-707 AND THR-708, ANDMASS SPECTROMETRY.	19131326 [Abstract]

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