dbPTM

GDE_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	GDE_HUMAN
UniProt AC	P35573
Protein Name	Glycogen debranching enzyme
Gene Name	AGL
Organism	Homo sapiens (Human).
Sequence Length	1532
Subcellular Localization	Cytoplasm . Under glycogenolytic conditions localizes to the nucleus.
Protein Description	Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation..
Protein Sequence	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
17	Ubiquitination	LLLNEMEKLEKTLFR HHHHHHHHHHHHHHH	61.71	-
29	Phosphorylation	LFRLEQGYELQFRLG HHHHHCCEEEEEEEC	17.11	-
38	Phosphorylation	LQFRLGPTLQGKAVT EEEEECCCCCCEEEE	29.89	26437602
56	Phosphorylation	NYPFPGETFNREKFR CCCCCCCCCCHHHHH	31.93	26437602
61	Ubiquitination	GETFNREKFRSLDWE CCCCCHHHHHCCCCC	42.72	-
64	Phosphorylation	FNREKFRSLDWENPT CCHHHHHCCCCCCCC	33.54	10571954
102	Phosphorylation	FLQGNEKSGGGYIVV EEECCCCCCCCEEEE	36.17	28152594
106	Phosphorylation	NEKSGGGYIVVDPIL CCCCCCCEEEECCCE	8.20	28152594
126	Glutathionylation	NHVLPLDCVTLQTFL CCEEECHHHHHHHHH	3.12	22555962
151	Ubiquitination	ESRLRVAKESGYNMI HHHHHHHHHHCCCEE	49.94	-
155	Phosphorylation	RVAKESGYNMIHFTP HHHHHHCCCEEECCC	15.73	22468782
161	Phosphorylation	GYNMIHFTPLQTLGL CCCEEECCCCHHHCC	14.66	22468782
169	Phosphorylation	PLQTLGLSRSCYSLA CCHHHCCCHHHHHHH	21.98	24719451
171	Phosphorylation	QTLGLSRSCYSLANQ HHHCCCHHHHHHHHH	17.65	27251275
173	Phosphorylation	LGLSRSCYSLANQLE HCCCHHHHHHHHHHC	14.15	27251275
174	Phosphorylation	GLSRSCYSLANQLEL CCCHHHHHHHHHHCC	25.90	27251275
191	Ubiquitination	DFSRPNRKYTWNDVG CCCCCCCCCCHHHHH	54.39	-
192	Phosphorylation	FSRPNRKYTWNDVGQ CCCCCCCCCHHHHHH	17.47	22673903
193	Phosphorylation	SRPNRKYTWNDVGQL CCCCCCCCHHHHHHH	22.34	26437602
265	2-Hydroxyisobutyrylation	SCDVAEGKYKEKGIP CCCCCCCCCHHCCCC	44.66	-
265	Acetylation	SCDVAEGKYKEKGIP CCCCCCCCCHHCCCC	44.66	26051181
265	Ubiquitination	SCDVAEGKYKEKGIP CCCCCCCCCHHCCCC	44.66	-
283	Phosphorylation	ENDHHMNSIRKIIWE CCCHHHHHHHHHHHH	19.05	-
309 (in isoform 2)	Ubiquitination	-	12.60	21890473
310 (in isoform 3)	Ubiquitination	-	6.75	21890473
322	Methylation	RLLTQENRRVTKSDP HHHHHHCCCCCCCCC	33.42	-
326	Ubiquitination	QENRRVTKSDPNQHL HHCCCCCCCCCCCCE	50.43	21906983
326 (in isoform 1)	Ubiquitination	-	50.43	21890473
327	Phosphorylation	ENRRVTKSDPNQHLT HCCCCCCCCCCCCEE	49.27	26437602
334	Phosphorylation	SDPNQHLTIIQDPEY CCCCCCEEEECCHHH	17.57	26437602
383	Phosphorylation	KRMEELNSEKHRLIN HHHHHHCHHHHHHHH	61.72	26437602
385	Acetylation	MEELNSEKHRLINYH HHHHCHHHHHHHHHC	33.57	27452117
415	Ubiquitination	RLAGHGPKLGPVTRK HHCCCCCCCCCCCCC	71.34	-
420	Phosphorylation	GPKLGPVTRKHPLVT CCCCCCCCCCCCCEE	36.81	-
475 (in isoform 2)	Ubiquitination	-	28.13	21890473
476 (in isoform 3)	Ubiquitination	-	34.31	21890473
479	Phosphorylation	AEPGSEVYLRRELIC CCCCCEEEEEEEEEE	7.16	-
492	Ubiquitination	ICWGDSVKLRYGNKP EECCCCEEECCCCCC	31.06	21890473
492 (in isoform 1)	Ubiquitination	-	31.06	21890473
495	Phosphorylation	GDSVKLRYGNKPEDC CCCEEECCCCCCCCC	35.17	26437602
504	Phosphorylation	NKPEDCPYLWAHMKK CCCCCCCCHHHHHHH	22.62	26437602
510	Acetylation	PYLWAHMKKYTEITA CCHHHHHHHCCCCCE	32.53	24885255
519	Phosphorylation	YTEITATYFQGVRLD CCCCCEEEECCEECC	7.46	26437602
567 (in isoform 2)	Phosphorylation	-	6.11	-
568 (in isoform 3)	Phosphorylation	-	2.37	-
575	Phosphorylation	VTRLGISSLIREAMS HHHHHHHHHHHHHHH	26.37	24719451
582	Phosphorylation	SLIREAMSAYNSHEE HHHHHHHHHCCCCCC	34.35	28796482
584	Phosphorylation	IREAMSAYNSHEEGR HHHHHHHCCCCCCCC	15.19	23401153
586	Phosphorylation	EAMSAYNSHEEGRLV HHHHHCCCCCCCCEE	21.43	27155012
638	Phosphorylation	PIVHRSAYDALPSTT CCCCHHHHHCCCCCE	11.73	-
662 (in isoform 2)	Ubiquitination	-	43.09	21890473
663 (in isoform 3)	Ubiquitination	-	7.11	21890473
672	Phosphorylation	PHQISVVSEERFYTK CCCEEEEECHHHCCC	31.42	26437602
679	Ubiquitination	SEERFYTKWNPEALP ECHHHCCCCCCHHCC	33.33	21906983
679 (in isoform 1)	Ubiquitination	-	33.33	21890473
706	Phosphorylation	IAARCAISKLHQELG HHHHHHHHHHHHHHC	15.61	30622161
707	Ubiquitination	AARCAISKLHQELGA HHHHHHHHHHHHHCC	43.82	-
738	Phosphorylation	AVTRHSPSIHQSVVA EEECCCCCHHHHHHH	35.40	26437602
747	Phosphorylation	HQSVVAVSRTAFRNP HHHHHHEECCCCCCC	17.74	24719451
771	Ubiquitination	PQMCIPGKIEEVVLE CCCCCCCCHHHHHHH	41.40	-
816	Acetylation	HIQLNESKIVKQAGV HHCCCCCHHHHHCCC	45.39	18530417
816	Ubiquitination	HIQLNESKIVKQAGV HHCCCCCHHHHHCCC	45.39	-
819	2-Hydroxyisobutyrylation	LNESKIVKQAGVATK CCCCHHHHHCCCCCC	37.59	-
819	Acetylation	LNESKIVKQAGVATK CCCCHHHHHCCCCCC	37.59	25953088
819	Ubiquitination	LNESKIVKQAGVATK CCCCHHHHHCCCCCC	37.59	-
826	Acetylation	KQAGVATKGPNEYIQ HHCCCCCCCCCHHEE	63.73	18530425
858 (in isoform 2)	Ubiquitination	-	4.90	21890473
859 (in isoform 3)	Ubiquitination	-	5.03	21890473
871	Phosphorylation	RNHLTQFSPHFKSGS HHHHHHCCCCCCCCC	13.98	26437602
872 (in isoform 2)	Ubiquitination	-	37.92	-
875	Acetylation	TQFSPHFKSGSLAVD HHCCCCCCCCCEECC	51.37	25953088
875	Ubiquitination	TQFSPHFKSGSLAVD HHCCCCCCCCCEECC	51.37	21890473
875 (in isoform 1)	Ubiquitination	-	51.37	21890473
876	Phosphorylation	QFSPHFKSGSLAVDN HCCCCCCCCCEECCC	32.44	24275569
878	Phosphorylation	SPHFKSGSLAVDNAD CCCCCCCCEECCCCC	21.90	-
889	Ubiquitination	DNADPILKIPFASLA CCCCCCHHCCHHHHH	49.40	-
894	Phosphorylation	ILKIPFASLASRLTL CHHCCHHHHHHHCHH	25.50	26437602
897	Phosphorylation	IPFASLASRLTLAEL CCHHHHHHHCHHHHH	32.87	24275569
978	Phosphorylation	SNRLISRSGTIAEVG HHHHHCCCCCHHHHH	33.23	26437602
980	Phosphorylation	RLISRSGTIAEVGKW HHHCCCCCHHHHHHH	20.47	26437602
1027	Phosphorylation	DTAWKQMSSFVQNGS HHHHHHHHHHHHCCC	20.38	22673903
1028	Phosphorylation	TAWKQMSSFVQNGST HHHHHHHHHHHCCCH	24.88	26437602
1060	Phosphorylation	FPSLPILSPALMDVP CCCCCCCCHHHHCCC	14.62	-
1060 (in isoform 2)	Ubiquitination	-	14.62	21890473
1075	Ubiquitination	YRLNEITKEKEQCCV CHHHHHHHCHHHHHH	72.89	-
1077	Ubiquitination	LNEITKEKEQCCVSL HHHHHHCHHHHHHHH	55.42	22053931
1130	O-linked_Glycosylation	IILAFAGTLRHGLIP HHHHHHHHHHCCCCH	19.72	30379171
1130	Phosphorylation	IILAFAGTLRHGLIP HHHHHHHHHHCCCCH	19.72	21406692
1245	Phosphorylation	TAGVDEETGFVYGGN CEEECCCCCCEECCC	33.59	-
1262	Ubiquitination	NCGTWMDKMGESDRA CCCCHHHHCCHHHHH	32.47	-
1277	Phosphorylation	RNRGIPATPRDGSAV HHCCCCCCCCCCCCE	17.32	21406692
1290	Phosphorylation	AVEIVGLSKSAVRWL CEEEEECCHHHHHHH	20.73	-
1291	Ubiquitination	VEIVGLSKSAVRWLL EEEEECCHHHHHHHH	47.69	-
1314	Acetylation	PYHEVTVKRHGKAIK CCCEEEEEECCEEEE	29.34	25953088
1314	Malonylation	PYHEVTVKRHGKAIK CCCEEEEEECCEEEE	29.34	26320211
1330	Ubiquitination	SYDEWNRKIQDNFEK CHHHHHHHHHHHHHH	41.32	-
1337	Ubiquitination	KIQDNFEKLFHVSED HHHHHHHHHHCCCCC	52.92	-
1351	Ubiquitination	DPSDLNEKHPNLVHK CHHHCCCCCCCHHHH	64.39	-
1358	Ubiquitination	KHPNLVHKRGIYKDS CCCCHHHHCCCCCCC	44.85	-
1365	Phosphorylation	KRGIYKDSYGASSPW HCCCCCCCCCCCCCC	22.70	28258704
1399	Ubiquitination	FTTEKAWKALEIAEK CCCHHHHHHHHHHHH	48.21	-
1406	Acetylation	KALEIAEKKLLGPLG HHHHHHHHHCCCCCC	39.43	2402129
1425	Glutathionylation	DPDDMVYCGIYDNAL CCCCCEEEEECCCCC	1.49	22555962
1464	Phosphorylation	YFLRAKLYFSRLMGP HHHHHHHHHHHHHCC	9.98	-
1466	Phosphorylation	LRAKLYFSRLMGPET HHHHHHHHHHHCCCC	15.79	-
1474	O-linked_Glycosylation	RLMGPETTAKTIVLV HHHCCCCCHHHHHHH	25.21	30379171

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	NHLRC1	Q6VVB1	PMID:17908927

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of GDE_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of GDE_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LGUL_HUMAN	GLO1	physical	22939629
NNRD_HUMAN	CARKD	physical	26344197
CATA_HUMAN	CAT	physical	26344197
FKB1A_HUMAN	FKBP1A	physical	26344197
FKB1B_HUMAN	FKBP1B	physical	26344197
GSH1_HUMAN	GCLC	physical	26344197
GRHPR_HUMAN	GRHPR	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
232400	Glycogen storage disease 3 (GSD3)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of GDE_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer."; Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.; Cell 131:1190-1203(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-584, AND MASSSPECTROMETRY.	18083107 [Abstract]