DHX58_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DHX58_HUMAN
• UniProt AC: Q96C10
• Protein Name: Probable ATP-dependent RNA helicase DHX58
• Gene Name: DHX58
• Organism: Homo sapiens (Human).
• Sequence Length: 678
• Subcellular Localization: Cytoplasm .
• Protein Description: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate..
• Protein Sequence: MELRSYQWEVIMPALEGKNIIIWLPTGAGKTRAAAYVAKRHLETVDGAKVVVLVNRVHLVTQHGEEFRRMLDGRWTVTTLSGDMGPRAGFGHLARCHDLLICTAELLQMALTSPEEEEHVELTVFSLIVVDECHHTHKDTVYNVIMSQYLELKLQRAQPLPQVLGLTASPGTGGASKLDGAINHVLQLCANLDTWCIMSPQNCCPQLQEHSQQPCKQYNLCHRRSQDPFGDLLKKLMDQIHDHLEMPELSRKFGTQMYEQQVVKLSEAAALAGLQEQRVYALHLRRYNDALLIHDTVRAVDALAALQDFYHREHVTKTQILCAERRLLALFDDRKNELAHLATHGPENPKLEMLEKILQRQFSSSNSPRGIIFTRTRQSAHSLLLWLQQQQGLQTVDIRAQLLIGAGNSSQSTHMTQRDQQEVIQKFQDGTLNLLVATSVAEEGLDIPHCNVVVRYGLLTNEISMVQARGRARADQSVYAFVATEGSRELKRELINEALETLMEQAVAAVQKMDQAEYQAKIRDLQQAALTKRAAQAAQRENQRQQFPVEHVQLLCINCMVAVGHGSDLRKVEGTHHVNVNPNFSNYYNVSRDPVVINKVFKDWKPGGVISCRNCGEVWGLQMIYKSVKLPVLKVRSMLLETPQGRIQAKKWSRVPFSVPDFDFLQHCAENLSDLSLD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
18	Acetylation	IMPALEGKNIIIWLP EEHHHCCCEEEEEEE	35.28	7429273
76	Phosphorylation	RMLDGRWTVTTLSGD HHHCCCEEEEEECCC	13.45	30576142
79	Phosphorylation	DGRWTVTTLSGDMGP CCCEEEEEECCCCCC	18.00	30576142
225	Phosphorylation	YNLCHRRSQDPFGDL HCCCCCCCCCHHHHH	38.60	27499020
310	Phosphorylation	LAALQDFYHREHVTK HHHHHHHHHHHCCCH	14.30	22817900
335	Acetylation	LALFDDRKNELAHLA HHHHCCCHHHHHHHH	62.86	7677899
350	Acetylation	THGPENPKLEMLEKI HHCCCCHHHHHHHHH	69.53	7677909
477	Phosphorylation	GRARADQSVYAFVAT CCCCCCCCEEEEEEC	20.04	25348954
479	Phosphorylation	ARADQSVYAFVATEG CCCCCCEEEEEECCC	10.28	25348954
484	Phosphorylation	SVYAFVATEGSRELK CEEEEEECCCCHHHH	35.26	25348954
518	Phosphorylation	QKMDQAEYQAKIRDL HCCCHHHHHHHHHHH	19.45	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of DHX58_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DHX58_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DHX58_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AGO2_HUMAN	AGO2	physical	21903422
DICER_HUMAN	DICER1	physical	21903422
E2AK2_HUMAN	EIF2AK2	physical	21903422
STAU1_HUMAN	STAU1	physical	21903422
STAU2_HUMAN	STAU2	physical	21903422
CARF_HUMAN	CDKN2AIP	physical	21903422
DHX30_HUMAN	DHX30	physical	21903422
IF6_HUMAN	EIF6	physical	21903422
NKRF_HUMAN	NKRF	physical	21903422
XRN2_HUMAN	XRN2	physical	21903422
A4_HUMAN	APP	physical	21832049

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.