dbPTM

NKRF_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NKRF_HUMAN
UniProt AC	O15226
Protein Name	NF-kappa-B-repressing factor
Gene Name	NKRF
Organism	Homo sapiens (Human).
Sequence Length	690
Subcellular Localization	Nucleus, nucleolus .
Protein Description	Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription..
Protein Sequence	MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	ILQMAEGIDIGEMPS HHHHHCCCCCCCCCC	2.34	24719451
11 (in isoform 2)	Phosphorylation	-	2.34	24719451
12	Phosphorylation	LQMAEGIDIGEMPSY HHHHCCCCCCCCCCC	54.41	24719451
12 (in isoform 2)	Phosphorylation	-	54.41	24719451
18	Phosphorylation	IDIGEMPSYDLVLSK CCCCCCCCCEEEECC	29.93	29978859
19	Phosphorylation	DIGEMPSYDLVLSKP CCCCCCCCEEEECCC	13.87	29978859
24	Phosphorylation	PSYDLVLSKPSKGQK CCCEEEECCCCCCCC	34.73	22210691
25	Acetylation	SYDLVLSKPSKGQKR CCEEEECCCCCCCCC	49.50	26051181
34	Phosphorylation	SKGQKRHLSTCDGQN CCCCCCCCCCCCCCC	5.42	27642862
35	Phosphorylation	KGQKRHLSTCDGQNP CCCCCCCCCCCCCCC	22.36	25159151
36	Phosphorylation	GQKRHLSTCDGQNPP CCCCCCCCCCCCCCC	22.45	23401153
40	Sumoylation	HLSTCDGQNPPKKQA CCCCCCCCCCCCCCC	46.11	-
40	Ubiquitination	HLSTCDGQNPPKKQA CCCCCCCCCCCCCCC	46.11	-
50	Acetylation	PKKQAGSKFHARPRF CCCCCCCCCCCCCCC	40.01	25953088
50	Phosphorylation	PKKQAGSKFHARPRF CCCCCCCCCCCCCCC	40.01	-
51	Phosphorylation	KKQAGSKFHARPRFE CCCCCCCCCCCCCCC	6.12	24719451
65	Phosphorylation	EPVHFVASSSKDERQ CCCEEECCCCCCCCC	29.82	25159151
66	Phosphorylation	PVHFVASSSKDERQE CCEEECCCCCCCCCC	31.50	30108239
67	Phosphorylation	VHFVASSSKDERQED CEEECCCCCCCCCCC	41.45	25159151
68	Sumoylation	HFVASSSKDERQEDP EEECCCCCCCCCCCC	66.61	-
68	Acetylation	HFVASSSKDERQEDP EEECCCCCCCCCCCC	66.61	25953088
68	Sumoylation	HFVASSSKDERQEDP EEECCCCCCCCCCCC	66.61	28112733
68	Ubiquitination	HFVASSSKDERQEDP EEECCCCCCCCCCCC	66.61	-
76	Phosphorylation	DERQEDPYGPQTKEV CCCCCCCCCCCHHHH	54.48	25159151
80	Phosphorylation	EDPYGPQTKEVNEQT CCCCCCCHHHHHHHH	32.10	-
82	Phosphorylation	PYGPQTKEVNEQTHF CCCCCHHHHHHHHCC	54.70	27251275
83	Sumoylation	YGPQTKEVNEQTHFA CCCCHHHHHHHHCCC	11.57	-
83	Ubiquitination	YGPQTKEVNEQTHFA CCCCHHHHHHHHCCC	11.57	-
87	Phosphorylation	TKEVNEQTHFASMPR HHHHHHHHCCCCCCC	16.87	23312004
91	Phosphorylation	NEQTHFASMPRDIYQ HHHHCCCCCCCCHHC	28.51	23312004
96	Sumoylation	FASMPRDIYQDYTQD CCCCCCCHHCCCCCC	3.34	-
96	Ubiquitination	FASMPRDIYQDYTQD CCCCCCCHHCCCCCC	3.34	-
129	Phosphorylation	LTKDQPVTANMYFDS EECCCCEEEEEECCC	20.99	30377224
133	Phosphorylation	QPVTANMYFDSGNPA CCEEEEEECCCCCCC	12.21	30377224
136	Phosphorylation	TANMYFDSGNPAPST EEEEECCCCCCCCCC	30.48	28348404
142	Phosphorylation	DSGNPAPSTTSQQAN CCCCCCCCCCCCCHH	46.56	28348404
143	Phosphorylation	SGNPAPSTTSQQANS CCCCCCCCCCCCHHC	29.43	28348404
144	Phosphorylation	GNPAPSTTSQQANSQ CCCCCCCCCCCHHCC	28.95	28348404
145	Phosphorylation	NPAPSTTSQQANSQS CCCCCCCCCCHHCCC	22.00	28348404
150	Phosphorylation	TTSQQANSQSTPEPS CCCCCHHCCCCCCCC	28.64	28348404
152	Phosphorylation	SQQANSQSTPEPSPS CCCHHCCCCCCCCCC	45.83	28348404
153	Phosphorylation	QQANSQSTPEPSPSQ CCHHCCCCCCCCCCC	24.84	28348404
157	Phosphorylation	SQSTPEPSPSQTFPE CCCCCCCCCCCCCCH	35.21	28348404
159	Phosphorylation	STPEPSPSQTFPESV CCCCCCCCCCCCHHH	46.30	28348404
186	Phosphorylation	ATIWKNLSNPEMTSG HHHHHHCCCCCCCCC	60.78	28464451
191	Phosphorylation	NLSNPEMTSGSDKIN HCCCCCCCCCCCCCC	28.42	28464451
191	Ubiquitination	NLSNPEMTSGSDKIN HCCCCCCCCCCCCCC	28.42	-
192	Phosphorylation	LSNPEMTSGSDKINY CCCCCCCCCCCCCCH	34.68	28464451
194	Phosphorylation	NPEMTSGSDKINYTY CCCCCCCCCCCCHHH	35.18	28464451
196	Ubiquitination	EMTSGSDKINYTYML CCCCCCCCCCHHHHH	34.25	-
198	Ubiquitination	TSGSDKINYTYMLTR CCCCCCCCHHHHHHH	28.91	-
204	Phosphorylation	INYTYMLTRCIQACK CCHHHHHHHHHHHHC	13.63	20068231
211	Ubiquitination	TRCIQACKTNPEYIY HHHHHHHCCCCHHEE	55.11	-
212	Phosphorylation	RCIQACKTNPEYIYA HHHHHHCCCCHHEEC	56.76	30576142
216	Phosphorylation	ACKTNPEYIYAPLKE HHCCCCHHEECCHHH	10.65	28509920
231	Phosphorylation	IPPADIPKNKKLLTD CCCCCCCCCCCCCCC	80.07	-
234	Ubiquitination	ADIPKNKKLLTDGYA CCCCCCCCCCCCCEE	59.11	-
237	Phosphorylation	PKNKKLLTDGYACEV CCCCCCCCCCEEEEE	37.50	21815630
237	Ubiquitination	PKNKKLLTDGYACEV CCCCCCCCCCEEEEE	37.50	-
240	Phosphorylation	KKLLTDGYACEVRCQ CCCCCCCEEEEEEEE	15.76	24732914
249	Ubiquitination	CEVRCQNIYLTTGYA EEEEEEEEEEECCCC	0.92	-
250	Phosphorylation	EVRCQNIYLTTGYAG EEEEEEEEEECCCCC	12.92	-
252	Phosphorylation	RCQNIYLTTGYAGSK EEEEEEEECCCCCCC	10.95	22817900
253	Phosphorylation	CQNIYLTTGYAGSKN EEEEEEECCCCCCCC	26.12	22817900
255	Phosphorylation	NIYLTTGYAGSKNGS EEEEECCCCCCCCCC	13.00	-
259	Ubiquitination	TTGYAGSKNGSRDRA ECCCCCCCCCCHHHH	65.49	-
267	Phosphorylation	NGSRDRATELAVKLL CCCHHHHHHHHHHHH	32.21	-
268	Phosphorylation	GSRDRATELAVKLLQ CCHHHHHHHHHHHHH	33.47	-
272	Acetylation	RATELAVKLLQKRIE HHHHHHHHHHHHHHH	37.56	26051181
274	Ubiquitination	TELAVKLLQKRIEVR HHHHHHHHHHHHHHH	4.71	-
287	Ubiquitination	VRVVRRKFKHTFGED HHHHHHHCCCCCCCC	7.13	-
288	Methylation	RVVRRKFKHTFGEDL HHHHHHCCCCCCCCE	44.78	115974049
291	Ubiquitination	RRKFKHTFGEDLVVC HHHCCCCCCCCEEEE	11.45	-
303	Methylation	VVCQIGMSSYEFPPA EEEEECCCCCCCCCC	25.26	-
325	Phosphorylation	VVLGKDASGQPIFNA EEEEECCCCCCCCCC	48.36	25159151
333	Phosphorylation	GQPIFNASAKHWTNF CCCCCCCCCCCCCCE	38.67	25159151
340	Phosphorylation	SAKHWTNFVITENAN CCCCCCCEEEECCCC	3.08	-
348	Phosphorylation	VITENANDAIGILNN EEECCCCHHHHHHCC	36.83	-
366	Phosphorylation	FNKMSIEYKYEMMPN CCCCCEEEEEEECCC	19.73	22817900
368	Phosphorylation	KMSIEYKYEMMPNRT CCCEEEEEEECCCCC	14.25	22817900
394	Acetylation	LAEGYGTKKTSKHAA HHCCCCCCCCCHHHH	50.09	26051181
407	Sumoylation	AAADEALKILQKTQP HHHHHHHHHHHHHCC	48.71	-
407	Acetylation	AAADEALKILQKTQP HHHHHHHHHHHHHCC	48.71	26051181
407	Ubiquitination	AAADEALKILQKTQP HHHHHHHHHHHHHCC	48.71	-
411	Ubiquitination	EALKILQKTQPTYPS HHHHHHHHHCCCCCC	45.76	-
413	Ubiquitination	LKILQKTQPTYPSVK HHHHHHHCCCCCCCC	35.06	-
421	Phosphorylation	PTYPSVKSSQCHTGS CCCCCCCHHCCCCCC	24.40	26552605
422	Phosphorylation	TYPSVKSSQCHTGSS CCCCCCHHCCCCCCC	30.98	29396449
422	Sumoylation	TYPSVKSSQCHTGSS CCCCCCHHCCCCCCC	30.98	-
422	Ubiquitination	TYPSVKSSQCHTGSS CCCCCCHHCCCCCCC	30.98	-
426	Phosphorylation	VKSSQCHTGSSPRGS CCHHCCCCCCCCCCC	46.51	30242111
426	Ubiquitination	VKSSQCHTGSSPRGS CCHHCCCCCCCCCCC	46.51	-
428	Phosphorylation	SSQCHTGSSPRGSGK HHCCCCCCCCCCCCC	38.02	25849741
429	Phosphorylation	SQCHTGSSPRGSGKK HCCCCCCCCCCCCCC	21.69	26329039
433	Phosphorylation	TGSSPRGSGKKKDIK CCCCCCCCCCCCCCC	49.03	29396449
435	Ubiquitination	SSPRGSGKKKDIKDL CCCCCCCCCCCCCCE	59.35	-
437	Phosphorylation	PRGSGKKKDIKDLVV CCCCCCCCCCCCEEE	69.08	17081983
444	Phosphorylation	KDIKDLVVYENSSNP CCCCCEEEEECCCCC	6.88	-
448	Phosphorylation	DLVVYENSSNPVCTL CEEEEECCCCCEEEE	21.39	29083192
449	Phosphorylation	LVVYENSSNPVCTLN EEEEECCCCCEEEEC	57.65	29083192
454	Phosphorylation	NSSNPVCTLNDTAQF CCCCCEEEECCCHHH	28.91	29083192
455	Sumoylation	SSNPVCTLNDTAQFN CCCCEEEECCCHHHH	4.64	-
458	Phosphorylation	PVCTLNDTAQFNRMT CEEEECCCHHHHHHH	22.68	29083192
464	Sulfoxidation	DTAQFNRMTVEYVYE CCHHHHHHHHHHHHH	5.30	28183972
486	Phosphorylation	KCKVILESEVIAEAV EEEEEEEEHHHHHHH	33.45	20068231
500	Sumoylation	VGVKKTVKYEAAGEA HCCCEEEEHHHCHHH	42.47	-
500	Acetylation	VGVKKTVKYEAAGEA HCCCEEEEHHHCHHH	42.47	26051181
500	Sumoylation	VGVKKTVKYEAAGEA HCCCEEEEHHHCHHH	42.47	25218447
501	Phosphorylation	GVKKTVKYEAAGEAV CCCEEEEHHHCHHHH	13.38	20068231
509	Acetylation	EAAGEAVKTLKKTQP HHCHHHHHHHHHCCC	56.01	26051181
510	Phosphorylation	AAGEAVKTLKKTQPT HCHHHHHHHHHCCCC	36.89	-
514	Phosphorylation	AVKTLKKTQPTVINN HHHHHHHCCCCHHHH	37.89	-
515	Sumoylation	VKTLKKTQPTVINNL HHHHHHCCCCHHHHH	40.31	-
517	Phosphorylation	TLKKTQPTVINNLKK HHHHCCCCHHHHHCC	24.97	-
523	Sumoylation	PTVINNLKKGAVEDV CCHHHHHCCCCHHHH	51.81	-
524	Sumoylation	TVINNLKKGAVEDVI CHHHHHCCCCHHHHH	56.09	-
524	Ubiquitination	TVINNLKKGAVEDVI CHHHHHCCCCHHHHH	56.09	-
528	Ubiquitination	NLKKGAVEDVISRNE HHCCCCHHHHHHHCH	46.13	-
532	Phosphorylation	GAVEDVISRNEIQGR CCHHHHHHHCHHCCC	28.97	20068231
538	Sumoylation	ISRNEIQGRSAEEAY HHHCHHCCCCHHHHH	31.04	-
538	Ubiquitination	ISRNEIQGRSAEEAY HHHCHHCCCCHHHHH	31.04	-
539	Sumoylation	SRNEIQGRSAEEAYK HHCHHCCCCHHHHHH	19.13	-
539	Ubiquitination	SRNEIQGRSAEEAYK HHCHHCCCCHHHHHH	19.13	-
546	Sumoylation	RSAEEAYKQQIKEDN CCHHHHHHHHHHHCC	42.65	-
546	Methylation	RSAEEAYKQQIKEDN CCHHHHHHHHHHHCC	42.65	110869363
550	Sumoylation	EAYKQQIKEDNIGNQ HHHHHHHHHCCHHHH	55.46	-
561	Sumoylation	IGNQLLRKMGWTGGG HHHHHHHHHCCCCCC	41.56	-
561	Methylation	IGNQLLRKMGWTGGG HHHHHHHHHCCCCCC	41.56	-
561	Sumoylation	IGNQLLRKMGWTGGG HHHHHHHHHCCCCCC	41.56	-
561	Ubiquitination	IGNQLLRKMGWTGGG HHHHHHHHHCCCCCC	41.56	-
565	Phosphorylation	LLRKMGWTGGGLGKS HHHHHCCCCCCCCCC	21.88	21406692
565	Sumoylation	LLRKMGWTGGGLGKS HHHHHCCCCCCCCCC	21.88	-
565	Ubiquitination	LLRKMGWTGGGLGKS HHHHHCCCCCCCCCC	21.88	-
571	Ubiquitination	WTGGGLGKSGEGIRE CCCCCCCCCCCCCCC	61.40	-
572	Phosphorylation	TGGGLGKSGEGIREP CCCCCCCCCCCCCCC	40.03	25159151
576	Sumoylation	LGKSGEGIREPISVK CCCCCCCCCCCCCHH	3.82	-
576	Ubiquitination	LGKSGEGIREPISVK CCCCCCCCCCCCCHH	3.82	-
580	Phosphorylation	GEGIREPISVKEQHK CCCCCCCCCHHHHHH	6.74	-
583	Sumoylation	IREPISVKEQHKREG CCCCCCHHHHHHHCC	44.88	-
587	Phosphorylation	ISVKEQHKREGLGLD CCHHHHHHHCCCCCC	52.41	27251275
598	Sumoylation	LGLDVERVNKIAKRD CCCCHHHHHHHHHHH	5.60	-
598	Ubiquitination	LGLDVERVNKIAKRD CCCCHHHHHHHHHHH	5.60	-
613	Phosphorylation	IEQIIRNYARSESHT HHHHHHHHHCCCCCC	8.11	28555341
616	Phosphorylation	IIRNYARSESHTDLT HHHHHHCCCCCCCCC	34.97	28450419
618	Phosphorylation	RNYARSESHTDLTFS HHHHCCCCCCCCCCC	32.69	25159151
620	Phosphorylation	YARSESHTDLTFSRE HHCCCCCCCCCCCCC	41.82	28387310
623	Phosphorylation	SESHTDLTFSRELTN CCCCCCCCCCCCCCH	23.52	20873877
625	Phosphorylation	SHTDLTFSRELTNDE CCCCCCCCCCCCHHH	21.43	20873877
631	Phosphorylation	FSRELTNDERKQIHQ CCCCCCHHHHHHHHH	52.41	-
633	Phosphorylation	RELTNDERKQIHQIA CCCCHHHHHHHHHHH	38.76	24719451
640	Phosphorylation	RKQIHQIAQKYGLKS HHHHHHHHHHHCCCC	8.01	-
642	Acetylation	QIHQIAQKYGLKSKS HHHHHHHHHCCCCCC	32.06	26051181
648	Acetylation	QKYGLKSKSHGVGHD HHHCCCCCCCCCCCC	44.90	12432303
649	Ubiquitination	KYGLKSKSHGVGHDR HHCCCCCCCCCCCCC	32.34	-
656	Methylation	SHGVGHDRYLVVGRK CCCCCCCCEEEECCH	22.47	115485071
657	Ubiquitination	HGVGHDRYLVVGRKR CCCCCCCEEEECCHH	15.20	-
666	Trimethylation	VVGRKRRKEDLLDQL EECCHHCHHHHHHHH	60.66	-
666	Methylation	VVGRKRRKEDLLDQL EECCHHCHHHHHHHH	60.66	-
666	Sumoylation	VVGRKRRKEDLLDQL EECCHHCHHHHHHHH	60.66	28112733
671	Methylation	RRKEDLLDQLKQEGQ HCHHHHHHHHHHCCC	60.09	-
674	Sumoylation	EDLLDQLKQEGQVGH HHHHHHHHHCCCCCE	40.44	-
674	Sumoylation	EDLLDQLKQEGQVGH HHHHHHHHHCCCCCE	40.44	28112733
681	Ubiquitination	KQEGQVGHYELVMPQ HHCCCCCEEEEECCC	17.65	-
682	Phosphorylation	QEGQVGHYELVMPQA HCCCCCEEEEECCCC	12.67	28796482
689	Sumoylation	YELVMPQAN------ EEEECCCCC------	20.16	-
689	Ubiquitination	YELVMPQAN------ EEEECCCCC------	20.16	-
697	Phosphorylation	N-------------- C--------------		27642862

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of NKRF_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NKRF_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NKRF_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TF65_HUMAN	RELA	physical	10562553
NFKB2_HUMAN	NFKB2	physical	10562553
NFKB1_HUMAN	NFKB1	physical	10562553
REL_HUMAN	REL	physical	10562553
DHX30_HUMAN	DHX30	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NKRF_HUMAN

Related Literatures of Post-Translational Modification