DDIT4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DDIT4_HUMAN
• UniProt AC: Q9NX09
• Protein Name: DNA damage-inducible transcript 4 protein
• Gene Name: DDIT4
• Organism: Homo sapiens (Human).
• Sequence Length: 232
• Subcellular Localization: Mitochondrion. Cytoplasm, cytosol .
• Protein Description: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death..
• Protein Sequence: MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MPSLWDRFSS -----CCCHHHHCCC	40.57	21955146
9	Phosphorylation	PSLWDRFSSSSTSSS CCHHHHCCCCCCCCC	30.15	21955146
10	Phosphorylation	SLWDRFSSSSTSSSP CHHHHCCCCCCCCCC	26.12	21955146
11	Phosphorylation	LWDRFSSSSTSSSPS HHHHCCCCCCCCCCC	36.12	21955146
12	Phosphorylation	WDRFSSSSTSSSPSS HHHCCCCCCCCCCCC	33.97	21955146
13	Phosphorylation	DRFSSSSTSSSPSSL HHCCCCCCCCCCCCC	34.28	21955146
14	Phosphorylation	RFSSSSTSSSPSSLP HCCCCCCCCCCCCCC	30.66	21955146
15	Phosphorylation	FSSSSTSSSPSSLPR CCCCCCCCCCCCCCC	46.48	21955146
16	Phosphorylation	SSSSTSSSPSSLPRT CCCCCCCCCCCCCCC	28.68	21955146
18	Phosphorylation	SSTSSSPSSLPRTPT CCCCCCCCCCCCCCC	46.59	21955146
19	Phosphorylation	STSSSPSSLPRTPTP CCCCCCCCCCCCCCC	45.86	21955146
23	Phosphorylation	SPSSLPRTPTPDRPP CCCCCCCCCCCCCCC	29.58	24114839
25	Phosphorylation	SSLPRTPTPDRPPRS CCCCCCCCCCCCCCC	36.89	24114839
121	Phosphorylation	PARLLMPSQLVSQVG CHHHHCHHHHHHHHH	23.37	20166753
129	Ubiquitination	QLVSQVGKELLRLAY HHHHHHHHHHHHHHH	46.46	21906983
188	Ubiquitination	LDSRLWPKIQGLFSS CCCCHHHHHHHHHHC	35.76	21906983
218	Ubiquitination	STGFRVIKKKLYSSE HCCCHHHHHHCCCCC	40.78	-
219	Ubiquitination	TGFRVIKKKLYSSEQ CCCHHHHHHCCCCCH	36.57	-
220	Ubiquitination	GFRVIKKKLYSSEQL CCHHHHHHCCCCCHH	47.88	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	PRKN	O60260	PMID:25101677
-	K	Ubiquitination	E3 ubiquitin ligase	BTRC	Q9Y297	PMID:19557001
-	K	Ubiquitination	E3 ubiquitin ligase	FBXW11	Q9UKB1	PMID:31406304

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DDIT4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DDIT4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LRIF1_HUMAN	LRIF1	physical	16169070
CUL4A_HUMAN	CUL4A	physical	19557001
DDB1_HUMAN	DDB1	physical	19557001
FBW1A_HUMAN	BTRC	physical	19557001
PRKN_HUMAN	PARK2	physical	25101677
HUWE1_HUMAN	HUWE1	physical	25147182
NEDD4_HUMAN	NEDD4	physical	27494837

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.