dbPTM

DBF4A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DBF4A_HUMAN
UniProt AC	Q9UBU7
Protein Name	Protein DBF4 homolog A
Gene Name	DBF4
Organism	Homo sapiens (Human).
Sequence Length	674
Subcellular Localization	Nucleus .
Protein Description	Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle..
Protein Sequence	MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	SGAMRIHSKGHFQGG CCCEEEECCCCCCCC	37.65	24719451
21	Sumoylation	FQGGIQVKNEKNRPS CCCCEEECCCCCCCC	42.91	-
21	Sumoylation	FQGGIQVKNEKNRPS CCCCEEECCCCCCCC	42.91	-
28	Phosphorylation	KNEKNRPSLKSLKTD CCCCCCCCHHHCCCC	45.23	29978859
30	Acetylation	EKNRPSLKSLKTDNR CCCCCCHHHCCCCCC	59.07	25953088
34	Phosphorylation	PSLKSLKTDNRPEKS CCHHHCCCCCCCCCC	43.74	22210691
52	Phosphorylation	PLWGKVFYLDLPSVT CCCCEEEEEECCCCC	11.42	29083192
57	Phosphorylation	VFYLDLPSVTISEKL EEEEECCCCCHHHHH	38.91	20873877
59	Phosphorylation	YLDLPSVTISEKLQK EEECCCCCHHHHHHH	24.11	20873877
61	Phosphorylation	DLPSVTISEKLQKDI ECCCCCHHHHHHHHH	21.22	29083192
81	Ubiquitination	RVEEFLSKDISYLIS HHHHHHCCCHHHHHC	62.32	-
84	Phosphorylation	EFLSKDISYLISNKK HHHCCCHHHHHCCCH	24.82	27135362
88	Phosphorylation	KDISYLISNKKEAKF CCHHHHHCCCHHHHH	39.75	27135362
98	Phosphorylation	KEAKFAQTLGRISPV HHHHHHHHHCCCCCC	27.96	23186163
103	Phosphorylation	AQTLGRISPVPSPES HHHHCCCCCCCCHHH	20.63	25849741
107	Phosphorylation	GRISPVPSPESAYTA CCCCCCCCHHHCCCC	41.35	20873877
110	Phosphorylation	SPVPSPESAYTAETT CCCCCHHHCCCCCCC	29.93	20873877
112	Phosphorylation	VPSPESAYTAETTSP CCCHHHCCCCCCCCC	18.76	20873877
113	Phosphorylation	PSPESAYTAETTSPH CCHHHCCCCCCCCCC	20.10	20873877
116	Phosphorylation	ESAYTAETTSPHPSH HHCCCCCCCCCCCCC	30.06	20873877
117	Phosphorylation	SAYTAETTSPHPSHD HCCCCCCCCCCCCCC	31.59	20873877
118	Phosphorylation	AYTAETTSPHPSHDG CCCCCCCCCCCCCCC	28.73	20873877
122	Phosphorylation	ETTSPHPSHDGSSFK CCCCCCCCCCCCCCC	31.04	20873877
126	Phosphorylation	PHPSHDGSSFKSPDT CCCCCCCCCCCCCCE	38.36	20873877
127	Phosphorylation	HPSHDGSSFKSPDTV CCCCCCCCCCCCCEE	42.07	20873877
130	Phosphorylation	HDGSSFKSPDTVCLS CCCCCCCCCCEEEEC	26.46	25159151
140	Ubiquitination	TVCLSRGKLLVEKAI EEEECCCCEEHHHHH	37.22	-
177	Phosphorylation	LHIDDIRYYIEQKKK EEHHHHHHHHHHHHC	14.77	-
187	Phosphorylation	EQKKKELYLLKKSST HHHHCEEEEEECCCC	15.87	30108239
194	Phosphorylation	YLLKKSSTSVRDGGK EEEECCCCCCEECCE	38.03	22817900
195	Phosphorylation	LLKKSSTSVRDGGKR EEECCCCCCEECCEE	19.66	22817900
201	Ubiquitination	TSVRDGGKRVGSGAQ CCCEECCEECCCCCC	49.82	-
205	Phosphorylation	DGGKRVGSGAQKTRT ECCEECCCCCCCCCC	28.26	29496963
210	O-linked_Glycosylation	VGSGAQKTRTGRLKK CCCCCCCCCCCCCCC	22.76	30379171
212	O-linked_Glycosylation	SGAQKTRTGRLKKPF CCCCCCCCCCCCCCE	31.70	30379171
259	Phosphorylation	PFDVDKPSSMQKQTQ CCCCCCCCHHCCCCE	44.15	25627689
260	Phosphorylation	FDVDKPSSMQKQTQV CCCCCCCHHCCCCEE	33.49	25159151
273	Phosphorylation	QVKLRIQTDGDKYGG EEEEEEECCCCCCCC	39.31	25159151
278	Phosphorylation	IQTDGDKYGGTSIQL EECCCCCCCCEEEEE	26.06	20873877
281	Phosphorylation	DGDKYGGTSIQLQLK CCCCCCCEEEEEEEH	20.49	20873877
282	Phosphorylation	GDKYGGTSIQLQLKE CCCCCCEEEEEEEHH	16.18	20873877
303	Phosphorylation	CECCLQKYEDLETHL HHHHHHHHHHHHHHH	11.17	-
312	Phosphorylation	DLETHLLSEQHRNFA HHHHHHHCHHHHHHH	41.38	25849741
341	Phosphorylation	LVFDFVEYEKDTPKK HHHHHHHCCCCCCCC	23.99	28450419
345	Phosphorylation	FVEYEKDTPKKKRIK HHHCCCCCCCCCCEE	48.34	30266825
353	Phosphorylation	PKKKRIKYSVGSLSP CCCCCEEEECCCCCC	13.25	28176443
354	Phosphorylation	KKKRIKYSVGSLSPV CCCCEEEECCCCCCC	18.26	25159151
357	Phosphorylation	RIKYSVGSLSPVSAS CEEEECCCCCCCCHH	24.42	30266825
359	Phosphorylation	KYSVGSLSPVSASVL EEECCCCCCCCHHHH	25.59	29255136
362	Phosphorylation	VGSLSPVSASVLKKT CCCCCCCCHHHHHHH	20.43	30266825
364	Phosphorylation	SLSPVSASVLKKTEQ CCCCCCHHHHHHHHC	22.07	30266825
381	Phosphorylation	KVELQHISQKDCQED HEEEEECCHHHCCCC	29.11	23401153
393	Ubiquitination	QEDDTTVKEQNFLYK CCCCCCHHHHCCEEC	52.03	-
408	Sumoylation	ETQETEKKLLFISEP CCHHHHHEEEEEECC	44.16	-
408	Sumoylation	ETQETEKKLLFISEP CCHHHHHEEEEEECC	44.16	-
413	Phosphorylation	EKKLLFISEPIPHPS HHEEEEEECCCCCCC	29.95	29255136
420	Phosphorylation	SEPIPHPSNELRGLN ECCCCCCCHHHHCHH	39.64	25159151
429	Ubiquitination	ELRGLNEKMSNKCSM HHHCHHHHHCCHHHH	46.70	-
431	Phosphorylation	RGLNEKMSNKCSMLS HCHHHHHCCHHHHHH	44.18	27732954
435	Phosphorylation	EKMSNKCSMLSTAED HHHCCHHHHHHCCHH	25.11	21815630
438	Phosphorylation	SNKCSMLSTAEDDIR CCHHHHHHCCHHHHH	19.55	28165663
439	Phosphorylation	NKCSMLSTAEDDIRQ CHHHHHHCCHHHHHH	30.06	21815630
449	Phosphorylation	DDIRQNFTQLPLHKN HHHHHHCCCCCCCCC	36.42	22123827
455	Ubiquitination	FTQLPLHKNKQECIL CCCCCCCCCHHEEEE	73.79	-
465	Phosphorylation	QECILDISEHTLSEN HEEEEECCCCCCCHH	24.00	27422710
468	Phosphorylation	ILDISEHTLSENDLE EEECCCCCCCHHHHH	28.11	20873877
470	Phosphorylation	DISEHTLSENDLEEL ECCCCCCCHHHHHHH	35.19	27422710
502	Phosphorylation	FSTDNSGSQPKQKSD CCCCCCCCCCCCCCC	43.03	22123827
508	Phosphorylation	GSQPKQKSDTVLFPA CCCCCCCCCEEEEEH	35.70	30266825
510	Phosphorylation	QPKQKSDTVLFPAKD CCCCCCCEEEEEHHH	26.63	30266825
535	Phosphorylation	THDSGLITINSSQEH ECCCCEEEECCCCCE	21.10	17525332
538	Phosphorylation	SGLITINSSQEHLTV CCEEEECCCCCEEEE	29.55	29523821
539	Phosphorylation	GLITINSSQEHLTVQ CEEEECCCCCEEEEE	34.79	17525332
544	Phosphorylation	NSSQEHLTVQAKAPF CCCCCEEEEEEECCC	16.69	29523821
553	Phosphorylation	QAKAPFHTPPEEPNE EEECCCCCCCCCCCC	40.70	23401153
568	Phosphorylation	CDFKNMDSLPSGKIH CCCCCCCCCCCCCHH	31.26	25159151
571	Phosphorylation	KNMDSLPSGKIHRKV CCCCCCCCCCHHCEE	58.99	29255136
573	Acetylation	MDSLPSGKIHRKVKI CCCCCCCCHHCEEEE	39.41	25953088
579	Sumoylation	GKIHRKVKIILGRNR CCHHCEEEEEECCCH	27.37	-
579	Sumoylation	GKIHRKVKIILGRNR CCHHCEEEEEECCCH	27.37	-
612	Phosphorylation	QEENRICSSPVQSLL HHHHCCCCCHHHHHH	34.99	20873877
613	Phosphorylation	EENRICSSPVQSLLD HHHCCCCCHHHHHHH	25.36	20873877
617	Phosphorylation	ICSSPVQSLLDLFQT CCCCHHHHHHHHHCC	31.00	27732954
624	Phosphorylation	SLLDLFQTSEEKSEF HHHHHHCCCHHHHHH	30.67	30177828
625	Phosphorylation	LLDLFQTSEEKSEFL HHHHHCCCHHHHHHC	32.73	20873877
629	Phosphorylation	FQTSEEKSEFLGFTS HCCCHHHHHHCCCCC	36.54	22777824
635	Phosphorylation	KSEFLGFTSYTEKSG HHHHCCCCCCCCCCC	21.01	28450419
636	Phosphorylation	SEFLGFTSYTEKSGI HHHCCCCCCCCCCCC	28.00	21815630
637	Phosphorylation	EFLGFTSYTEKSGIC HHCCCCCCCCCCCCC	18.83	28450419
638	Phosphorylation	FLGFTSYTEKSGICN HCCCCCCCCCCCCCE	36.38	28450419
664	Phosphorylation	NLLTAFFSSPSTSTF CCCHHHHCCCCCCCC	34.58	-
665	Phosphorylation	LLTAFFSSPSTSTFT CCHHHHCCCCCCCCC	20.08	-
667	Phosphorylation	TAFFSSPSTSTFTGF HHHHCCCCCCCCCCC	36.62	29997176
669	Phosphorylation	FFSSPSTSTFTGF-- HHCCCCCCCCCCC--	26.34	29997176

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
273	T	Phosphorylation	Kinase	CDC7	O00311	PSP
281	T	Phosphorylation	Kinase	CDC7	O00311	PSP
312	S	Phosphorylation	Kinase	CDC7	O00311	PSP
449	T	Phosphorylation	Kinase	ATM	Q13315	PSP
449	T	Phosphorylation	Kinase	ATR	Q13535	PSP
502	S	Phosphorylation	Kinase	ATM	Q13315	PSP
502	S	Phosphorylation	Kinase	ATR	Q13535	PSP
539	S	Phosphorylation	Kinase	ATM	Q13315	PSP
539	S	Phosphorylation	Kinase	ATR	Q13535	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of DBF4A_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DBF4A_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MCM2_HUMAN	MCM2	physical	12614612
ORC1_HUMAN	ORC1	physical	12614612
ORC2_HUMAN	ORC2	physical	12614612
ORC5_HUMAN	ORC5	physical	12614612
ORC6_HUMAN	ORC6	physical	12614612
MCM3_HUMAN	MCM3	physical	12614612
MCM4_HUMAN	MCM4	physical	12614612
MCM7_HUMAN	MCM7	physical	12614612
ORC4_HUMAN	ORC4	physical	12614612
CDC7_HUMAN	CDC7	physical	10523313
CDC7_HUMAN	CDC7	physical	10373557
CDC7_HUMAN	CDC7	physical	16912045
DBF4A_HUMAN	DBF4	physical	12968034
DAXX_HUMAN	DAXX	physical	12968034
MCM2_HUMAN	MCM2	physical	10846177
CDC7_HUMAN	CDC7	physical	10846177
DBF4A_HUMAN	DBF4	physical	10846177
MCM4_HUMAN	MCM4	physical	10846177
MCM6_HUMAN	MCM6	physical	10846177
MCM7_HUMAN	MCM7	physical	10846177
RAD18_HUMAN	RAD18	physical	24240236
HDGR2_HUMAN	HDGFRP2	physical	28514442
CDC7_HUMAN	CDC7	physical	28514442
NDK6_HUMAN	NME6	physical	28514442
EF1A2_HUMAN	EEF1A2	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DBF4A_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-273; THR-345; SER-359;SER-381; SER-413 AND SER-508, AND MASS SPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354 AND SER-359, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359 AND SER-381, ANDMASS SPECTROMETRY.	18691976 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381; THR-535 ANDSER-539, AND MASS SPECTROMETRY.	17525332 [Abstract]