CRK1_SCHPO - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CRK1_SCHPO
• UniProt AC: Q12126
• Protein Name: Serine/threonine-protein kinase crk1
• Gene Name: crk1
• Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
• Sequence Length: 335
• Subcellular Localization: Cytoplasm . Nucleus .
• Protein Description: Protein kinase essential for cell proliferation, where it is required for completion of cytokinesis. Phosphorylates the C-terminal repeat domain (CTD) of RNA polymerase II..
• Protein Sequence: MDIEKSDKWTYVKERKVGEGTYAVVFLGRQKETNRRVAIKKIKVGQFKDGIDISALREIKFLRESRHDNVIELVDVFSTKSNLNIILEFLDSDLEMLIKDKFIVFQPAHIKSWMVMLLRGLHHIHSRFILHRDLKPNNLLISSDGVLKLADFGLSRDFGTPSHMSHQVITRWYRPPELFMGCRSYGTGVDMWSVGCIFAELMLRTPYLPGESDLDQLNVIFRALGTPEPEVIKSMQQLPNYVEMKHIPPPNGGMEALFSAAGHEEIDLLKMMLDYNPYRRPTAQQALEHHYFSALPKPTHPSLLPRKGGEEGIKHVSSDLQRQNNFPMRANIKFV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
160	Phosphorylation	GLSRDFGTPSHMSHQ CCCCCCCCCCHHCHH	22.93	29996109
162	Phosphorylation	SRDFGTPSHMSHQVI CCCCCCCCHHCHHHH	31.49	28889911
165	Phosphorylation	FGTPSHMSHQVITRW CCCCCHHCHHHHHCC	12.82	9857180
170	Phosphorylation	HMSHQVITRWYRPPE HHCHHHHHCCCCCHH	19.12	25720772
317	Phosphorylation	EEGIKHVSSDLQRQN HHHHHHHHHHHHHHC	20.18	29996109
318	Phosphorylation	EGIKHVSSDLQRQNN HHHHHHHHHHHHHCC	41.05	28889911

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
165	S	Phosphorylation	Kinase	CDK7	-	GPS
165	S	Phosphorylation	Kinase	CAK	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CRK1_SCHPO !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CRK1_SCHPO !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CSK1_SCHPO	csk1	genetic	9857180
CSK1_SCHPO	csk1	genetic	11226158
CDK1_SCHPO	cdc2	genetic	2474475
SEP1_SCHPO	sep1	genetic	15829570
CGM2_SCHPO	mcs2	physical	9857180
CGM2_SCHPO	mcs2	physical	10226032
CGM2_SCHPO	mcs2	physical	15555586
TFB3_SCHPO	pmh1	physical	15555586
RPB1_SCHPO	rpb1	physical	20605454
SPB1_SCHPO	spb1	physical	22508988
CDK9_SCHPO	cdk9	genetic	22876190
MTO1_SCHPO	SPBC30B4.06c	genetic	22681890
MCL1_SCHPO	mcl1	genetic	22681890
CDK9_SCHPO	cdk9	genetic	26275777
RPB1_SCHPO	rpb1	physical	25691663
CDK1_SCHPO	cdc2	physical	25691663

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of fission yeast.";
Wilson-Grady J.T., Villen J., Gygi S.P.;
J. Proteome Res. 7:1088-1097(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162; SER-165 ANDSER-318, AND MASS SPECTROMETRY.
PubMed: 18257517

"Fission yeast Csk1 is a CAK-activating kinase (CAKAK).";
Hermand D., Pihlak A., Westerling T., Damagnez V., Vandenhaute J.,Cottarel G., Makela T.P.;
EMBO J. 17:7230-7238(1998).
Cited for: PHOSPHORYLATION AT SER-165.
PubMed: 9857180