CASP3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CASP3_MOUSE
• UniProt AC: P70677
• Protein Name: Caspase-3
• Gene Name: Casp3
• Organism: Mus musculus (Mouse).
• Sequence Length: 277
• Subcellular Localization: Cytoplasm.
• Protein Description: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes..
• Protein Sequence: MENNKTSVDSKSINNFEVKTIHGSKSVDSGIYLDSSYKMDYPEMGICIIINNKNFHKSTGMSSRSGTDVDAANLRETFMGLKYQVRNKNDLTREDILELMDSVSKEDHSKRSSFVCVILSHGDEGVIYGTNGPVELKKLTSFFRGDYCRSLTGKPKLFIIQACRGTELDCGIETDSGTDEEMACQKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCSMLKLYAHKLEFMHILTRVNRKVATEFESFSLDSTFHAKKQIPCIVSMLTKELYFYH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MENNKTSV -------CCCCCCCC	12.88	-
6	Phosphorylation	--MENNKTSVDSKSI --CCCCCCCCCCCCC	35.79	26745281
7	Phosphorylation	-MENNKTSVDSKSIN -CCCCCCCCCCCCCC	26.07	29514104
10	Phosphorylation	NNKTSVDSKSINNFE CCCCCCCCCCCCCEE	26.58	23984901
11	Acetylation	NKTSVDSKSINNFEV CCCCCCCCCCCCEEE	51.39	23806337
12	Phosphorylation	KTSVDSKSINNFEVK CCCCCCCCCCCEEEE	34.20	23684622
19	Ubiquitination	SINNFEVKTIHGSKS CCCCEEEEEEECCCC	34.27	22790023
19	Acetylation	SINNFEVKTIHGSKS CCCCEEEEEEECCCC	34.27	22826441
20	Phosphorylation	INNFEVKTIHGSKSV CCCEEEEEEECCCCC	24.31	-
25	Ubiquitination	VKTIHGSKSVDSGIY EEEEECCCCCCCCEE	60.12	22790023
25	Acetylation	VKTIHGSKSVDSGIY EEEEECCCCCCCCEE	60.12	23806337
26	Phosphorylation	KTIHGSKSVDSGIYL EEEECCCCCCCCEEE	32.62	25521595
29	Phosphorylation	HGSKSVDSGIYLDSS ECCCCCCCCEEECCC	25.57	25521595
57	Ubiquitination	INNKNFHKSTGMSSR ECCCCCCCCCCCCCC	45.64	22790023
65	Phosphorylation	STGMSSRSGTDVDAA CCCCCCCCCCCCCHH	48.25	24759943
82	Ubiquitination	RETFMGLKYQVRNKN HHHHHHHHEEECCCC	28.10	22790023
82	Acetylation	RETFMGLKYQVRNKN HHHHHHHHEEECCCC	28.10	22826441
154	Acetylation	YCRSLTGKPKLFIIQ CHHHHCCCCEEEEEE	33.47	22826441
156	Acetylation	RSLTGKPKLFIIQAC HHHCCCCEEEEEEEC	60.56	22826441
163	S-nitrosylation	KLFIIQACRGTELDC EEEEEEECCCCCCCC	2.06	-
163	Glutathionylation	KLFIIQACRGTELDC EEEEEEECCCCCCCC	2.06	24333276
163	S-nitrosocysteine	KLFIIQACRGTELDC EEEEEEECCCCCCCC	2.06	-
166	Phosphorylation	IIQACRGTELDCGIE EEEECCCCCCCCEEE	17.64	26525534
174	Phosphorylation	ELDCGIETDSGTDEE CCCCEEECCCCCCHH	33.28	26525534
176	Phosphorylation	DCGIETDSGTDEEMA CCEEECCCCCCHHHH	51.52	26525534
178	Phosphorylation	GIETDSGTDEEMACQ EEECCCCCCHHHHHC	44.16	26525534
229	Acetylation	MLKLYAHKLEFMHIL HHHHHHHHHHHHHHH	41.35	22826441
245	Phosphorylation	RVNRKVATEFESFSL HHHHHHHHCCCCCCC	44.16	22871156
255	Phosphorylation	ESFSLDSTFHAKKQI CCCCCCCCCCHHCHH	21.07	28576409
260	Acetylation	DSTFHAKKQIPCIVS CCCCCHHCHHHHHHH	55.00	22826441

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CASP3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CASP3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CASP3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EAA2_MOUSE	Slc1a2	physical	17823119
PSME3_MOUSE	Psme3	physical	11859414
FEM1B_HUMAN	FEM1B	physical	10542291
DFFA_MOUSE	Dffa	physical	15572351
ROCK1_MOUSE	Rock1	physical	18723680
GDIR1_MOUSE	Arhgdia	physical	18723680
BID_MOUSE	Bid	physical	18723680
CTNB1_MOUSE	Ctnnb1	physical	18723680
DFFB_MOUSE	Dffb	physical	15572351
IL16_MOUSE	Il16	physical	10479680

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteomic analysis of the developing mouse brain.";
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
Mol. Cell. Proteomics 3:1093-1101(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, AND MASSSPECTROMETRY.
PubMed: 15345747