BECN1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BECN1_MOUSE
• UniProt AC: O88597
• Protein Name: Beclin-1
• Gene Name: Becn1
• Organism: Mus musculus (Mouse).
• Sequence Length: 448
• Subcellular Localization: Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein
• Protein Description: Plays a central role in autophagy. [PubMed: 10604474]
• Protein Sequence: MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEEANSGEEPFIETRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQVRYAQIQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEGSKASS -------CCCCCCCC	13.69	-
7	Phosphorylation	-MEGSKASSSTMQVS -CCCCCCCCCCCEEE	28.67	29472430
8	Phosphorylation	MEGSKASSSTMQVSF CCCCCCCCCCCEEEE	34.39	29472430
9	Phosphorylation	EGSKASSSTMQVSFV CCCCCCCCCCEEEEE	25.96	29472430
10	Phosphorylation	GSKASSSTMQVSFVC CCCCCCCCCEEEEEE	17.36	29472430
14	Phosphorylation	SSSTMQVSFVCQRCS CCCCCEEEEEECCCC	8.62	-
28	Phosphorylation	SQPLKLDTSFKILDR CCCCCCCCCEEEECE	46.64	23984901
29	Phosphorylation	QPLKLDTSFKILDRV CCCCCCCCEEEECEE	24.98	26824392
56	Phosphorylation	AQAKPGETQEEEANS CCCCCCCCHHHHHHC	47.39	23140645
63	Phosphorylation	TQEEEANSGEEPFIE CHHHHHHCCCCCCEE	55.41	28066266
71	Phosphorylation	GEEPFIETRQDGVSR CCCCCEEECCCCCCC	28.90	29472430
88	Phosphorylation	IPPARMMSTESANSF CCCHHHCCCCCCCCE	21.49	26643407
89	Phosphorylation	PPARMMSTESANSFT CCHHHCCCCCCCCEE	18.96	26643407
91	Phosphorylation	ARMMSTESANSFTLI HHHCCCCCCCCEEEE	32.60	26643407
94	Phosphorylation	MSTESANSFTLIGEA CCCCCCCCEEEEEEC	21.23	26643407
102	Phosphorylation	FTLIGEASDGGTMEN EEEEEECCCCCCHHH	32.27	17242355
117	Phosphorylation	LSRRLKVTGDLFDIM HHHHHHHCCCHHHHH	24.32	-
175	Phosphorylation	EQMNEDDSEQLQREL HHHCCCCHHHHHHHH	39.29	20495213
392	Phosphorylation	ETRFCLPYRMDVEKG CCEEEEEEEEEEECC	12.96	-
428	Ubiquitination	EQWTKALKFMLTNLK HHHHHHHHHHHHHHH	33.32	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
14	S	Phosphorylation	Kinase	ULK1	O70405	PSP
14	S	Phosphorylation	Kinase	ULK2	Q9QY01	PSP
88	S	Phosphorylation	Kinase	AMPK	-	Uniprot
91	S	Phosphorylation	Kinase	AMPK	-	Uniprot
94	S	Phosphorylation	Kinase	AMPK	-	Uniprot
117	T	Phosphorylation	Kinase	DAPK1	Q80YE7	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
11	K	ubiquitylation	Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages (By similarity).	28445460
117	T	Phosphorylation	Phosphorylation at Thr-117 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy (By similarity).	23332761
400	K	ubiquitylation	'Lys-48'-linked poyubiquitination of Lys-400 leads to degradation.	28445460
400	K	ubiquitylation	Polyubiquitinated at Lys-400 with 'Lys-48'-linkages.	28445460

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BECN1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PRKN_MOUSE	Park2	physical	23737459
TCAM1_MOUSE	Ticam1	physical	18772134
MYD88_MOUSE	Myd88	physical	18772134
BCL2_MOUSE	Bcl2	physical	18772134
BCL2_MOUSE	Bcl2	physical	28347844

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.