dbPTM

BAD_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	BAD_MOUSE
UniProt AC	Q61337
Protein Name	Bcl2-associated agonist of cell death
Gene Name	Bad
Organism	Mus musculus (Mouse).
Sequence Length	204
Subcellular Localization	Mitochondrion outer membrane. Cytoplasm . Colocalizes with HIF3A isoform 2 in the cytoplasm (PubMed:21546903). Upon phosphorylation, locates to the cytoplasm.
Protein Description	Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways..
Protein Sequence	MGTPKQPSLAPAHALGLRKSDPGIRSLGSDAGGRRWRPAAQSMFQIPEFEPSEQEDASATDRGLGPSLTEDQPGPYLAPGLLGSNIHQQGRAATNSHHGGAGAMETRSRHSSYPAGTEEDEGMEEELSPFRGRSRSAPPNLWAAQRYGRELRRMSDEFEGSFKGLPRPKSAGTATQMRQSAGWTRIIQSWWDRNLGKGGSTPSQ

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
20	Phosphorylation	HALGLRKSDPGIRSL HHHCCCCCCCCHHHC	42.47	24719451
26	Phosphorylation	KSDPGIRSLGSDAGG CCCCCHHHCCCCCCC	34.85	-
29	Phosphorylation	PGIRSLGSDAGGRRW CCHHHCCCCCCCCCC	29.58	22871156
67	Phosphorylation	TDRGLGPSLTEDQPG CCCCCCCCCCCCCCC	46.23	-
76	Phosphorylation	TEDQPGPYLAPGLLG CCCCCCCCCCCCCCC	22.78	22817900
94	Phosphorylation	HQQGRAATNSHHGGA HHCCCHHCCCCCCCC	35.95	22817900
96	Phosphorylation	QGRAATNSHHGGAGA CCCHHCCCCCCCCCC	16.71	22817900
108	Phosphorylation	AGAMETRSRHSSYPA CCCCCCCCCCCCCCC	41.59	26239621
111	Phosphorylation	METRSRHSSYPAGTE CCCCCCCCCCCCCCC	30.38	27742792
112	Phosphorylation	ETRSRHSSYPAGTEE CCCCCCCCCCCCCCC	29.07	27087446
113	Phosphorylation	TRSRHSSYPAGTEED CCCCCCCCCCCCCCC	10.50	27742792
117	Phosphorylation	HSSYPAGTEEDEGME CCCCCCCCCCCCCCC	38.00	27742792
123	Oxidation	GTEEDEGMEEELSPF CCCCCCCCCCCCCCC	5.72	17242355
128	Phosphorylation	EGMEEELSPFRGRSR CCCCCCCCCCCCCCC	25.85	25168779
131	Asymmetric dimethylarginine	EEELSPFRGRSRSAP CCCCCCCCCCCCCCC	42.89	-
131	Methylation	EEELSPFRGRSRSAP CCCCCCCCCCCCCCC	42.89	-
133	Asymmetric dimethylarginine	ELSPFRGRSRSAPPN CCCCCCCCCCCCCCC	25.33	-
133	Methylation	ELSPFRGRSRSAPPN CCCCCCCCCCCCCCC	25.33	-
134	Phosphorylation	LSPFRGRSRSAPPNL CCCCCCCCCCCCCCH	33.15	27087446
136	Phosphorylation	PFRGRSRSAPPNLWA CCCCCCCCCCCCHHH	46.50	27087446
155	Phosphorylation	GRELRRMSDEFEGSF HHHHHHCCHHCCCCC	31.98	27087446
161	Phosphorylation	MSDEFEGSFKGLPRP CCHHCCCCCCCCCCC	19.31	25619855
170	Phosphorylation	KGLPRPKSAGTATQM CCCCCCCCCCHHHHH	34.31	25521595
173	Phosphorylation	PRPKSAGTATQMRQS CCCCCCCHHHHHHHH	26.67	26643407
175	Phosphorylation	PKSAGTATQMRQSAG CCCCCHHHHHHHHHC	23.80	26643407
180	Phosphorylation	TATQMRQSAGWTRII HHHHHHHHHCCHHHH	20.55	29514104
201	Phosphorylation	NLGKGGSTPSQ---- CCCCCCCCCCC----	30.18	16166315

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
26	S	Phosphorylation	Kinase	IKKB	O88351	PSP
94	T	Phosphorylation	Kinase	PIM3	P58750	PSP
96	S	Phosphorylation	Kinase	PIM3	P58750	PSP
112	S	Phosphorylation	Kinase	PIM3	Q86V86	PSP
112	S	Phosphorylation	Kinase	RPS6KA2	Q9WUT3	GPS
112	S	Phosphorylation	Kinase	RPS6KA3	P18654	GPS
112	S	Phosphorylation	Kinase	RPS6KA5	Q8C050	GPS
112	S	Phosphorylation	Kinase	MAPK8	Q91Y86	GPS
112	S	Phosphorylation	Kinase	PAK1	Q13153	PSP
112	S	Phosphorylation	Kinase	PAK1	O88643	Uniprot
112	S	Phosphorylation	Kinase	AKT1	P31749	PSP
112	S	Phosphorylation	Kinase	PAK4	Q8BTW9	Uniprot
112	S	Phosphorylation	Kinase	PAK5	Q8C015	Uniprot
112	S	Phosphorylation	Kinase	PIM1	P11309	PSP
112	S	Phosphorylation	Kinase	PIM1	P06803	PSP
112	S	Phosphorylation	Kinase	PIM2	Q9P1W9	PSP
112	S	Phosphorylation	Kinase	PIM2	Q62070	Uniprot
112	S	Phosphorylation	Kinase	PAK2	Q8CIN4	Uniprot
112	S	Phosphorylation	Kinase	PIM3	P58750	Uniprot
112	S	Phosphorylation	Kinase	RAF1	P04049	PSP
112	S	Phosphorylation	Kinase	RAF1	Q99N57	Uniprot
112	S	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
112	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
112	S	Phosphorylation	Kinase	RAF-FAMILY	-	GPS
112	S	Phosphorylation	Kinase	RSK-SUBFAMILY	-	GPS
112	S	Phosphorylation	Kinase	PKA	-	Uniprot
112	S	Phosphorylation	Kinase	PKB_GROUP	-	PhosphoELM
112	S	Phosphorylation	Kinase	RSK_GROUP	-	PhosphoELM
112	S	Phosphorylation	Kinase	P90RSK	P18653	PSP
112	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
112	S	Phosphorylation	Kinase	AKT1	P31750	PSP
112	S	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
112	S	Phosphorylation	Kinase	PKACA	P17612	PSP
112	S	Phosphorylation	Kinase	PKACA	P00517	PSP
117	T	Phosphorylation	Kinase	CSK21	Q60737	PhosphoELM
117	T	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
128	S	Phosphorylation	Kinase	MAPK8	Q91Y86	GPS
128	S	Phosphorylation	Kinase	CDK1	P06493	PSP
128	S	Phosphorylation	Kinase	CDK1	P11440	PSP
136	S	Phosphorylation	Kinase	PIM2	Q9P1W9	PSP
136	S	Phosphorylation	Kinase	AKT1	P31749	PSP
136	S	Phosphorylation	Kinase	PKACA	P17612	PSP
136	S	Phosphorylation	Kinase	PIM3	Q86V86	PSP
136	S	Phosphorylation	Kinase	PKACA	P00517	PSP
136	S	Phosphorylation	Kinase	PIM3	P58750	PSP
136	S	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
136	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
136	S	Phosphorylation	Kinase	RAF-FAMILY	-	GPS
136	S	Phosphorylation	Kinase	AKT1	P31750	PSP
136	S	Phosphorylation	Kinase	PKA	-	Uniprot
136	S	Phosphorylation	Kinase	PKB_GROUP	-	PhosphoELM
136	S	Phosphorylation	Kinase	PIM1	P06803	PSP
136	S	Phosphorylation	Kinase	PAK1	Q13153	PSP
136	S	Phosphorylation	Kinase	PRKCQ	Q02111	Uniprot
136	S	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
136	S	Phosphorylation	Kinase	PAK1	O88643	Uniprot
136	S	Phosphorylation	Kinase	PAK4	Q8BTW9	GPS
136	S	Phosphorylation	Kinase	RPS6KB1	Q8BSK8	Uniprot
136	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
136	S	Phosphorylation	Kinase	RPS6KA1	P18653	Uniprot
136	S	Phosphorylation	Kinase	PIM1	P11309	PSP
155	S	Phosphorylation	Kinase	PKA	-	Uniprot
155	S	Phosphorylation	Kinase	RPS6KA5	O75582	GPS
155	S	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
155	S	Phosphorylation	Kinase	RSK-SUBFAMILY	-	GPS
155	S	Phosphorylation	Kinase	RSK_GROUP	-	PhosphoELM
155	S	Phosphorylation	Kinase	RAF-FAMILY	-	GPS
155	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
155	S	Phosphorylation	Kinase	AKT1	P31750	PSP
155	S	Phosphorylation	Kinase	PIM2	Q9P1W9	PSP
155	S	Phosphorylation	Kinase	P90RSK	P18653	PSP
155	S	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
155	S	Phosphorylation	Kinase	PRKG1	P0C605	GPS
155	S	Phosphorylation	Kinase	PKG1	Q13976	PSP
155	S	Phosphorylation	Kinase	PIM3	P58750	PSP
155	S	Phosphorylation	Kinase	PKACA	P00517	PSP
155	S	Phosphorylation	Kinase	PIM1	P11309	PSP
155	S	Phosphorylation	Kinase	PIM3	Q86V86	PSP
155	S	Phosphorylation	Kinase	PKACA	P17612	PSP
155	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
170	S	Phosphorylation	Kinase	CAMK2G	Q13555	PSP
170	S	Phosphorylation	Kinase	PIM3	P58750	PSP
173	T	Phosphorylation	Kinase	PIM3	P58750	PSP
201	T	Phosphorylation	Kinase	MAPK8	Q91Y86	GPS
201	T	Phosphorylation	Kinase	JNK1	P45983	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
112	S	Phosphorylation	9381178
Phosphorylated on one or more of Ser-112, Ser-136, Ser-155 and Ser-170 in response to survival stimuli, which blocks its pro-apoptotic activity.
112	S	Phosphorylation	9381178
Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins.
131	R	Methylation	10611223
Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136.
131	R	Phosphorylation	10611223
Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136.
133	R	Methylation	10611223
Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136.
133	R	Phosphorylation	10611223
Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136.
136	S	Methylation	9381178
Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136.
136	S	Phosphorylation	9381178
Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136.
136	S	Phosphorylation	9381178
Phosphorylated on one or more of Ser-112, Ser-136, Ser-155 and Ser-170 in response to survival stimuli, which blocks its pro-apoptotic activity.
136	S	Phosphorylation	9381178
Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins.
136	S	Phosphorylation	9381178
Ser-136 is the major site of AKT/PKB phosphorylation, Ser-155 the major site of protein kinase A (CAPK) phosphorylation.
155	S	Phosphorylation	11717309
Phosphorylated on one or more of Ser-112, Ser-136, Ser-155 and Ser-170 in response to survival stimuli, which blocks its pro-apoptotic activity.
155	S	Phosphorylation	11717309
Ser-136 is the major site of AKT/PKB phosphorylation, Ser-155 the major site of protein kinase A (CAPK) phosphorylation.
155	S	Phosphorylation	11717309
This interaction then facilitates the phosphorylation at Ser-155, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival.
170	S	Phosphorylation	11717309
Phosphorylated on one or more of Ser-112, Ser-136, Ser-155 and Ser-170 in response to survival stimuli, which blocks its pro-apoptotic activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of BAD_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SODM_MOUSE	Sod2	physical	12931191
KAPCA_MOUSE	Prkaca	physical	12931191
WASF1_MOUSE	Wasf1	physical	12931191
HXK4_MOUSE	Gck	physical	12931191
BCL2_MOUSE	Bcl2	physical	21084607
B2CL1_MOUSE	Bcl2l1	physical	21084607

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of BAD_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, AND MASSSPECTROMETRY.	17242355 [Abstract]
"p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1regulates its mitochondrial localization, phosphorylation of BAD, andBcl-2 association."; Jin S., Zhuo Y., Guo W., Field J.; J. Biol. Chem. 280:24698-24705(2005). Cited for: PHOSPHORYLATION AT SER-112 BY RAF1.	15849194 [Abstract]
"The PIM-2 kinase phosphorylates BAD on serine 112 and reverses BAD-induced cell death."; Yan B., Zemskova M., Holder S., Chin V., Kraft A., Koskinen P.J.,Lilly M.; J. Biol. Chem. 278:45358-45367(2003). Cited for: PHOSPHORYLATION AT SER-112 BY PIM2.	12954615 [Abstract]
"Identification of a novel phosphorylation site, Ser-170, as aregulator of bad pro-apoptotic activity."; Dramsi S., Scheid M.P., Maiti A., Hojabrpour P., Chen X., Schubert K.,Goodlett D.R., Aebersold R., Duronio V.; J. Biol. Chem. 277:6399-6405(2002). Cited for: PHOSPHORYLATION AT SER-170, MUTAGENESIS OF SER-112; SER-155 ANDSER-170, AND MASS SPECTROMETRY.	11717309 [Abstract]
"p70S6 kinase signals cell survival as well as growth, inactivatingthe pro-apoptotic molecule BAD."; Harada H., Andersen J.S., Mann M., Terada N., Korsmeyer S.J.; Proc. Natl. Acad. Sci. U.S.A. 98:9666-9670(2001). Cited for: PHOSPHORYLATION AT SER-136.	11493700 [Abstract]
"Protein kinase C-theta mediates a selective T cell survival signalvia phosphorylation of BAD."; Villalba M., Bushway P., Altman A.; J. Immunol. 166:5955-5963(2001). Cited for: PHOSPHORYLATION AT SER-136.	11342610 [Abstract]
"p21-activated protein kinase gamma-PAK suppresses programmed celldeath of BALB3T3 fibroblasts."; Jakobi R., Moertl E., Koeppel M.A.; J. Biol. Chem. 276:16624-16634(2001). Cited for: PHOSPHORYLATION AT SER-112.	11278362 [Abstract]
"p21-activated kinase 1 phosphorylates the death agonist bad andprotects cells from apoptosis."; Schurmann A., Mooney A.F., Sanders L.C., Sells M.A., Wang H.G.,Reed J.C., Bokoch G.M.; Mol. Cell. Biol. 20:453-461(2000). Cited for: PHOSPHORYLATION AT SER-112 AND SER-136.	10611223 [Abstract]
"Rsk1 mediates a MEK-MAP kinase cell survival signal."; Shimamura A., Ballif B.A., Richards S.A., Blenis J.; Curr. Biol. 10:127-135(2000). Cited for: PHOSPHORYLATION AT SER-112 AND SER-136.	10679322 [Abstract]
"Interleukin-3-induced phosphorylation of BAD through the proteinkinase Akt."; Del Peso L., Gonzalez-Garcia M., Page C., Herrera R., Nunez G.; Science 278:687-689(1997). Cited for: PHOSPHORYLATION AT SER-112 AND SER-136, AND MUTAGENESIS OF SER-112 ANDSER-136.	9381178 [Abstract]

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