AXIN1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AXIN1_RAT
• UniProt AC: O70239
• Protein Name: Axin-1
• Gene Name: Axin1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 827
• Subcellular Localization: Cytoplasm . Nucleus . Cell membrane . Membrane . On UV irradiation, translocates to the nucleus and colocalizes with DAAX (By similarity). MACF1 is required for its translocation to cell membrane (By similarity).
• Protein Description: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (By similarity). Also facilitates the phosphorylation of APC by GSK3B (By similarity). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (By similarity). Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity)..
• Protein Sequence: MNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDSVPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHAPKLGLKLDSAGLHHHRHVHHHVHHNSARPKEQMEAEAARRVQSSFSWGPETHGHAKPRSYSESTGTNPSAGDLAFGGKASAPSKRNTKKAESGKNASAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGMRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAILPVFEEKIIGKVEKVD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
64	Phosphorylation	STATPRRSDLDLGYE CCCCCCHHHCCCCCC	43.75	28689409
70	Phosphorylation	RSDLDLGYEPEGSAS HHHCCCCCCCCCCCC	35.29	28689409
75	Phosphorylation	LGYEPEGSASPTPPY CCCCCCCCCCCCCCH	24.74	22108457
77	Phosphorylation	YEPEGSASPTPPYLR CCCCCCCCCCCCHHH	30.88	28689409
79	Phosphorylation	PEGSASPTPPYLRWA CCCCCCCCCCHHHHH	34.11	23984901
82	Phosphorylation	SASPTPPYLRWAESL CCCCCCCHHHHHHHH	15.31	28689409
160	Phosphorylation	NGIVSRQTKPATKSF CCCCCCCCCCCCHHH	37.57	21738781
164	Phosphorylation	SRQTKPATKSFIKDC CCCCCCCCHHHHHHH	35.43	21738781
166	Phosphorylation	QTKPATKSFIKDCVM CCCCCCHHHHHHHHH	28.05	21738781
217	Phosphorylation	YTRTGSESPKVCSDQ EEECCCCCCCEECCC	31.55	-
222	Phosphorylation	SESPKVCSDQSSGSG CCCCCEECCCCCCCC	41.49	25575281
225	Phosphorylation	PKVCSDQSSGSGTGK CCEECCCCCCCCCCC	41.48	25575281
226	Phosphorylation	KVCSDQSSGSGTGKG CEECCCCCCCCCCCC	31.20	25575281
228	Phosphorylation	CSDQSSGSGTGKGMS ECCCCCCCCCCCCCC	34.82	25575281
230	Phosphorylation	DQSSGSGTGKGMSGY CCCCCCCCCCCCCCC	37.10	25575281
275	Phosphorylation	TQKLLLETAAPRAPS HHHHHHHHCCCCCCC	28.26	16641100
282	Phosphorylation	TAAPRAPSSRRYNEG HCCCCCCCCCCCCCC	35.18	16641100
283	Phosphorylation	AAPRAPSSRRYNEGR CCCCCCCCCCCCCCC	21.51	16641100
317	Phosphorylation	YALAPATSANDSEQQ CEECCCCCCCHHHHH	27.47	-
321	Phosphorylation	PATSANDSEQQSLSS CCCCCCHHHHHHCCC	36.79	-
325	Phosphorylation	ANDSEQQSLSSDADT CCHHHHHHCCCCCCC	29.88	-
468	Phosphorylation	AHEENPESILDEHVQ HHHHCHHHHHHHHHH	29.70	-
480	Phosphorylation	HVQRVMRTPGCQSPG HHHHHHCCCCCCCCC	13.11	27097102
485	Phosphorylation	MRTPGCQSPGPGHRS HCCCCCCCCCCCCCC	35.58	27097102
492	Phosphorylation	SPGPGHRSPDSGHVA CCCCCCCCCCCCCEE	27.64	27097102
495	Phosphorylation	PGHRSPDSGHVAKTA CCCCCCCCCCEEEEE	33.89	27097102
509	Phosphorylation	AVLGGTASGHGKHAP EECCCCCCCCCCCCC	31.03	-
578	Phosphorylation	KPRSYSESTGTNPSA CCCCCCCCCCCCCCC	26.62	-
617	Phosphorylation	NASAEVPSTTEDAEK CCCCCCCCCCCHHHH	53.81	28689409
618	Phosphorylation	ASAEVPSTTEDAEKN CCCCCCCCCCHHHHH	28.30	28689409
619	Phosphorylation	SAEVPSTTEDAEKNQ CCCCCCCCCHHHHHH	35.88	28689409
787	Phosphorylation	TKKGSYRYYFKKVSD HHCCCEEEEEEEECC	12.67	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
75	S	Phosphorylation	Kinase	CK1	-	Uniprot
77	S	Phosphorylation	Kinase	CK1	-	Uniprot
217	S	Phosphorylation	Kinase	CK1	-	Uniprot
468	S	Phosphorylation	Kinase	CK1	-	Uniprot
480	T	Phosphorylation	Kinase	GSK3-BETA	P18266	Uniprot
485	S	Phosphorylation	Kinase	GSK3-BETA	P18266	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
858	K	Sumoylation	Sumoylation at Lys-858 and Lys-861 prevents ubiquitination and degradation.	-
858	K	ubiquitylation	Sumoylation at Lys-858 and Lys-861 prevents ubiquitination and degradation.	-
861	K	Sumoylation	Sumoylation at Lys-858 and Lys-861 prevents ubiquitination and degradation.	-
861	K	ubiquitylation	Sumoylation at Lys-858 and Lys-861 prevents ubiquitination and degradation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AXIN1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AXIN1_RAT	Axin1	physical	10330181
DVL3_HUMAN	DVL3	physical	10330181
GSK3B_HUMAN	GSK3B	physical	10330181
CTNB1_HUMAN	CTNNB1	physical	10330181
DVL1_HUMAN	DVL1	physical	10330181
APC_HUMAN	APC	physical	10330181
DVL1_RAT	Dvl1	physical	11113207

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.