dbPTM

ARH_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ARH_HUMAN
UniProt AC	Q5SW96
Protein Name	Low density lipoprotein receptor adapter protein 1 {ECO:0000305}
Gene Name	LDLRAP1 {ECO:0000312\|HGNC:HGNC:18640}
Organism	Homo sapiens (Human).
Sequence Length	308
Subcellular Localization	Cytoplasm .
Protein Description	Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression (By similarity)..
Protein Sequence	MDALKSAGRALIRSPSLAKQSWGGGGRHRKLPENWTDTRETLLEGMLFSLKYLGMTLVEQPKGEELSAAAIKRIVATAKASGKKLQKVTLKVSPRGIILTDNLTNQLIENVSIYRISYCTADKMHDKVFAYIAQSQHNQSLECHAFLCTKRKMAQAVTLTVAQAFKVAFEFWQVSKEEKEKRDKASQEGGDVLGARQDCTPSLKSLVATGNLLDLEETAKAPLSTVSANTTNMDEVPRPQALSGSSVVWELDDGLDEAFSRLAQSRTNPQVLDTGLTAQDMHYAQCLSPVDWDKPDSSGTEQDDLFSF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDALKSAG -------CHHHHHHH	6.49	22814378
5	Methylation	---MDALKSAGRALI ---CHHHHHHHHHHH	39.72	115972471
5	Ubiquitination	---MDALKSAGRALI ---CHHHHHHHHHHH	39.72	-
14	Phosphorylation	AGRALIRSPSLAKQS HHHHHHCCHHHHCCC	16.31	29255136
16	Phosphorylation	RALIRSPSLAKQSWG HHHHCCHHHHCCCCC	42.05	29255136
19	Ubiquitination	IRSPSLAKQSWGGGG HCCHHHHCCCCCCCC	50.59	-
49	Phosphorylation	LLEGMLFSLKYLGMT HHHHHHHHHHHHCCE	21.71	24719451
67	Phosphorylation	QPKGEELSAAAIKRI CCCCHHCCHHHHHHH	21.09	-
72	Ubiquitination	ELSAAAIKRIVATAK HCCHHHHHHHHHHHH	31.20	-
79	Ubiquitination	KRIVATAKASGKKLQ HHHHHHHHHCCCCEE	38.44	-
81	Phosphorylation	IVATAKASGKKLQKV HHHHHHHCCCCEEEE	50.90	30576142
93	Phosphorylation	QKVTLKVSPRGIILT EEEEEEECCCEEEEE	13.80	23917254
184	Ubiquitination	EEKEKRDKASQEGGD HHHHHHHHHHHHCCC	54.71	21906983
186	Phosphorylation	KEKRDKASQEGGDVL HHHHHHHHHHCCCCC	34.21	17525332
202	Phosphorylation	ARQDCTPSLKSLVAT CCCCCCHHHHHHHHH	30.21	-
205	Phosphorylation	DCTPSLKSLVATGNL CCCHHHHHHHHHCCC	33.11	28348404
274	Phosphorylation	TNPQVLDTGLTAQDM CCCCHHCCCCCHHHC	29.90	-
277	Phosphorylation	QVLDTGLTAQDMHYA CHHCCCCCHHHCCHH	24.50	27251275
283	Phosphorylation	LTAQDMHYAQCLSPV CCHHHCCHHHHCCCC	7.54	27642862
288	Phosphorylation	MHYAQCLSPVDWDKP CCHHHHCCCCCCCCC	30.42	26074081
297	Phosphorylation	VDWDKPDSSGTEQDD CCCCCCCCCCCCCCC	38.91	22210691

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ARH_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ARH_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ARH_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LRP2_HUMAN	LRP2	physical	14528014
CLH1_HUMAN	CLTC	physical	12221107
AP2B1_HUMAN	AP2B1	physical	12221107
LDLR_HUMAN	LDLR	physical	12221107
KLC1_HUMAN	KLC1	physical	21915095
STN1_HUMAN	OBFC1	physical	25416956
NEUL_HUMAN	NLN	physical	26186194
CHK1_HUMAN	CHEK1	physical	26186194
CC85C_HUMAN	CCDC85C	physical	26186194
DCA16_HUMAN	DCAF16	physical	26186194
NEUL_HUMAN	NLN	physical	28514442
CC85C_HUMAN	CCDC85C	physical	28514442
CHK1_HUMAN	CHEK1	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
603813	Hypercholesterolemia, autosomal recessive (ARH)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ARH_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, AND MASSSPECTROMETRY.	18669648 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND MASSSPECTROMETRY.	17525332 [Abstract]