ARBK1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ARBK1_MOUSE
• UniProt AC: Q99MK8
• Protein Name: Beta-adrenergic receptor kinase 1
• Gene Name: Grk2 {ECO:0000312|MGI:MGI:87940}
• Organism: Mus musculus (Mouse).
• Sequence Length: 689
• Subcellular Localization: Cytoplasm . Cell membrane .
• Protein Description: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity..
• Protein Sequence: MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEAKPLVEFYEEIKKYEKLETEEERVVRSREIFDSYIMKELLACSHPFSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKRPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIVHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLIQRGSANGL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
13	Phosphorylation	AVLADVSYLMAMEKS HHHHHHHHHHHHHHC	10.59	18369319
72	S-nitrosocysteine	YLLFRDFCLNHLEEA HHHHHHHHHHHHHHH	4.33	-
72	S-nitrosylation	YLLFRDFCLNHLEEA HHHHHHHHHHHHHHH	4.33	22178444
86	Phosphorylation	AKPLVEFYEEIKKYE HHHHHHHHHHHHHHH	10.11	18369319
92	Phosphorylation	FYEEIKKYEKLETEE HHHHHHHHHCCCCHH	17.19	18369319
120	S-nitrosocysteine	IMKELLACSHPFSKN HHHHHHHCCCCCCCC	3.76	-
120	Glutathionylation	IMKELLACSHPFSKN HHHHHHHCCCCCCCC	3.76	24333276
120	S-nitrosylation	IMKELLACSHPFSKN HHHHHHHCCCCCCCC	3.76	22178444
340	S-nitrosocysteine	ISDLGLACDFSKKRP HHHHCEECCCCCCCC	6.98	-
340	S-nitrosylation	ISDLGLACDFSKKRP HHHHCEECCCCCCCC	6.98	22178444
344	Ubiquitination	GLACDFSKKRPHASV CEECCCCCCCCCCCC	53.44	-
356	Phosphorylation	ASVGTHGYMAPEVLQ CCCCCCCCCCHHHHH	5.19	-
389	Phosphorylation	FKLLRGHSPFRQHKT HHHHHCCCHHHHCCC	28.67	-
439	S-nitrosylation	DVNRRLGCLGRGAQE HHHHHHCCCCCCCHH	4.27	22178444
439	S-nitrosocysteine	DVNRRLGCLGRGAQE HHHHHHCCCCCCCHH	4.27	-
487	Phosphorylation	ADAFDIGSFDEEDTK HHCCCCCCCCCCCCC	29.83	22817900
493	Phosphorylation	GSFDEEDTKGIKLLD CCCCCCCCCCEEEEC	34.15	30635358
501	Phosphorylation	KGIKLLDSDQELYRN CCEEEECCCHHHHHH	40.96	27357545
553	Phosphorylation	QLGHEEDYALGKDCI CCCCHHHHHCCCCEE	13.65	29514104
564	Phosphorylation	KDCIVHGYMSKMGNP CCEEHHHHHHHCCCC	5.37	25195567
619	S-nitrosocysteine	TQIKERKCLLLKIRG HHCCCCEEEEEEEEC	3.97	-
619	S-nitrosylation	TQIKERKCLLLKIRG HHCCCCEEEEEEEEC	3.97	22178444
670	Phosphorylation	KMKNKPRSPVVELSK HHCCCCCCCCEEHHC	30.49	25521595
676	Phosphorylation	RSPVVELSKVPLIQR CCCCEEHHCCCEEEC	20.36	27566939
685	Phosphorylation	VPLIQRGSANGL--- CCEEECCCCCCC---	22.28	25266776

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ARBK1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ARBK1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ARBK1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MDM2_MOUSE	Mdm2	physical	21081496
SCNNB_MOUSE	Scnn1b	physical	17544362
NEDD4_MOUSE	Nedd4	physical	17544362
GBB1_MOUSE	Gnb1	physical	7972043
GBG2_MOUSE	Gng2	physical	7972043

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.