dbPTM

ADDB_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ADDB_HUMAN
UniProt AC	P35612
Protein Name	Beta-adducin
Gene Name	ADD2
Organism	Homo sapiens (Human).
Sequence Length	726
Subcellular Localization	Cytoplasm, cytoskeleton. Cell membrane Peripheral membrane protein Cytoplasmic side.
Protein Description	Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit..
Protein Sequence	MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQLTDQELEEYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEEGTKKTETSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSEETVPEA ------CCCCCCCCC	44.11	-
2	Phosphorylation	------MSEETVPEA ------CCCCCCCCC	44.11	23025827
5	Phosphorylation	---MSEETVPEAASP ---CCCCCCCCCCCC	36.99	22199227
11 (in isoform 3)	Phosphorylation	-	45.01	-
11 (in isoform 2)	Phosphorylation	-	45.01	-
11	Phosphorylation	ETVPEAASPPPPQGQ CCCCCCCCCCCCCCC	45.01	28348404
14 (in isoform 9)	Phosphorylation	-	36.91	22210691
18 (in isoform 9)	Phosphorylation	-	25.03	22210691
20	Phosphorylation	PPPQGQPYFDRFSED CCCCCCCCHHCCCCC	15.38	28450419
25	Phosphorylation	QPYFDRFSEDDPEYM CCCHHCCCCCCHHHH	40.80	28450419
31	Phosphorylation	FSEDDPEYMRLRNRA CCCCCHHHHHHHHHH	7.91	24927040
36 (in isoform 9)	Phosphorylation	-	42.40	22210691
55	Phosphorylation	MEQKKRVTMILQSPS HHHHHHEEEECCCHH	11.52	21406692
60	Phosphorylation	RVTMILQSPSFREEL HEEEECCCHHHHHHH	21.04	29691806
62	Phosphorylation	TMILQSPSFREELEG EEECCCHHHHHHHHH	42.03	29691806
176	Phosphorylation	LISPKGVSCSEVTAS EECCCCCCCCCCCHH	22.16	25307156
178	Phosphorylation	SPKGVSCSEVTASSL CCCCCCCCCCCHHHC	27.82	23403867
184	Phosphorylation	CSEVTASSLIKVNIL CCCCCHHHCEEEEEC	31.48	24719451
344	Phosphorylation	HRPHEVGSVQWAGST CCCCCCCCCEECCCC	19.13	-
377	Phosphorylation	RMLDNLGYRTGYTYR HHHHHHCCCCCCCCC	14.25	43014423
392	Phosphorylation	HPFVQEKTKHKSEVE CCCCCCCCCCCCCCC	37.31	24719451
396	Phosphorylation	QEKTKHKSEVEIPAT CCCCCCCCCCCCCEE	46.74	21406692
403	Phosphorylation	SEVEIPATVTAFVFE CCCCCCEEEEEEEEC	17.03	21406692
405	Phosphorylation	VEIPATVTAFVFEED CCCCEEEEEEEECCC	15.26	21406692
436	Phosphorylation	EKTRWLNTPNTYLRV HHHHHCCCCCCEEEE	18.75	23025827
439	Phosphorylation	RWLNTPNTYLRVNVA HHCCCCCCEEEEEHH	25.74	29978859
440	Phosphorylation	WLNTPNTYLRVNVAD HCCCCCCEEEEEHHH	10.11	30804005
455	Phosphorylation	EVQRSMGSPRPKTTW HHHHHCCCCCCCCCC	14.75	27422710
464	Acetylation	RPKTTWMKADEVEKS CCCCCCEEHHHCEEC	44.22	7676393
471	Phosphorylation	KADEVEKSSSGMPIR EHHHCEECCCCCCEE	19.07	23403867
472	Phosphorylation	ADEVEKSSSGMPIRI HHHCEECCCCCCEEE	41.81	23403867
489	Phosphorylation	PNQFVPLYTDPQEVL CCCEEECCCCHHHHH	11.74	28796482
490	Phosphorylation	NQFVPLYTDPQEVLE CCEEECCCCHHHHHH	49.22	28796482
512	Phosphorylation	QNRQDVKSAGPQSQL HHHHHHHHHCHHHHH	38.00	31437679
522	Phosphorylation	PQSQLLASVIAEKSR HHHHHHHHHHHHHCC	17.64	23025827
528	Phosphorylation	ASVIAEKSRSPSTES HHHHHHHCCCCCHHH	29.51	20363803
528 (in isoform 2)	Phosphorylation	-	29.51	24260401
530 (in isoform 2)	Phosphorylation	-	30.23	24719451
530	Phosphorylation	VIAEKSRSPSTESQL HHHHHCCCCCHHHHH	30.23	28787133
532 (in isoform 3)	Phosphorylation	-	46.16	-
532	Phosphorylation	AEKSRSPSTESQLMS HHHCCCCCHHHHHHH	46.16	19664994
533	Phosphorylation	EKSRSPSTESQLMSK HHCCCCCHHHHHHHC	42.94	4076659
535	Phosphorylation	SRSPSTESQLMSKGD CCCCCHHHHHHHCCC	28.80	21082442
539	Phosphorylation	STESQLMSKGDEDTK CHHHHHHHCCCCCCC	41.92	20363803
543 (in isoform 8)	Phosphorylation	-	76.01	24260401
544 (in isoform 9)	Phosphorylation	-	64.89	24260401
545	Phosphorylation	MSKGDEDTKDDSEET HHCCCCCCCCCCCCC	34.03	23286773
545 (in isoform 8)	Phosphorylation	-	34.03	24719451
546 (in isoform 9)	Phosphorylation	-	72.38	24719451
549	Phosphorylation	DEDTKDDSEETVPNP CCCCCCCCCCCCCCH	47.68	23286773
552	Phosphorylation	TKDDSEETVPNPFSQ CCCCCCCCCCCHHHH	36.89	24076635
561	Phosphorylation	PNPFSQLTDQELEEY CCHHHHCCHHHHHHH	28.18	15345747
561 (in isoform 3)	Phosphorylation	-	28.18	-
568	Phosphorylation	TDQELEEYKKEVERK CHHHHHHHHHHHHHH	20.27	26471730
576	Acetylation	KKEVERKKLELDGEK HHHHHHHHCCCCCCC	54.11	20167786
583	Acetylation	KLELDGEKETAPEEP HCCCCCCCCCCCCCC	66.93	20167786
585	Phosphorylation	ELDGEKETAPEEPGS CCCCCCCCCCCCCCC	59.59	23927012
592	Phosphorylation	TAPEEPGSPAKSAPA CCCCCCCCCCCCCCC	32.19	23927012
595	Acetylation	EEPGSPAKSAPASPV CCCCCCCCCCCCCCC	51.00	7367709
596	Phosphorylation	EPGSPAKSAPASPVQ CCCCCCCCCCCCCCC	41.41	22617229
600	Phosphorylation	PAKSAPASPVQSPAK CCCCCCCCCCCCCCC	25.25	22617229
604	Phosphorylation	APASPVQSPAKEAET CCCCCCCCCCCCCCC	27.36	19413330
611	Phosphorylation	SPAKEAETKSPLVSP CCCCCCCCCCCCCCC	43.82	23898821
613	Phosphorylation	AKEAETKSPLVSPSK CCCCCCCCCCCCCCC	30.77	22617229
617	Phosphorylation	ETKSPLVSPSKSLEE CCCCCCCCCCCCCCC	31.01	22617229
619	Phosphorylation	KSPLVSPSKSLEEGT CCCCCCCCCCCCCCC	27.63	22617229
621	Phosphorylation	PLVSPSKSLEEGTKK CCCCCCCCCCCCCCC	45.40	28787133
626	Phosphorylation	SKSLEEGTKKTETSK CCCCCCCCCCCCCCC	32.42	28450419
631	Phosphorylation	EGTKKTETSKAATTE CCCCCCCCCCCCCCC	40.53	62150401
632	Phosphorylation	GTKKTETSKAATTEP CCCCCCCCCCCCCCC	17.67	62150407
657	O-linked_Glycosylation	NGREEEQTAEEILSK CCCCCHHHHHHHHHH	39.04	30379171
663	O-linked_Glycosylation	QTAEEILSKGLSQMT HHHHHHHHHHHHHHC	30.27	30379171
667	Phosphorylation	EILSKGLSQMTTSAD HHHHHHHHHHCCCCC	26.95	22210691
671	Phosphorylation	KGLSQMTTSADTDVD HHHHHHCCCCCCCCC	19.30	22210691
672	Phosphorylation	GLSQMTTSADTDVDT HHHHHCCCCCCCCCC	18.25	27732954
675	Phosphorylation	QMTTSADTDVDTSKD HHCCCCCCCCCCCCC	37.32	30576142
679	Phosphorylation	SADTDVDTSKDKTES CCCCCCCCCCCCCCC	37.21	22210691
681	Acetylation	DTDVDTSKDKTESVT CCCCCCCCCCCCCCC	66.39	7676407
683	Acetylation	DVDTSKDKTESVTSG CCCCCCCCCCCCCCC	58.91	7676415
684	Phosphorylation	VDTSKDKTESVTSGP CCCCCCCCCCCCCCC	43.56	28450419
686	Phosphorylation	TSKDKTESVTSGPMS CCCCCCCCCCCCCCC	35.61	28450419
688	Phosphorylation	KDKTESVTSGPMSPE CCCCCCCCCCCCCCC	36.93	23927012
689	Phosphorylation	DKTESVTSGPMSPEG CCCCCCCCCCCCCCC	38.45	23927012
693	Phosphorylation	SVTSGPMSPEGSPSK CCCCCCCCCCCCCCC	23.96	23927012
697	Phosphorylation	GPMSPEGSPSKSPSK CCCCCCCCCCCCCCC	25.08	23927012
699	Phosphorylation	MSPEGSPSKSPSKKK CCCCCCCCCCCCCCC	47.68	23927012
701	Phosphorylation	PEGSPSKSPSKKKKK CCCCCCCCCCCCCCC	38.45	19664994
703	Phosphorylation	GSPSKSPSKKKKKFR CCCCCCCCCCCCCCC	65.29	23927012
711	Phosphorylation	KKKKKFRTPSFLKKS CCCCCCCCHHHHHHH	26.81	30266825
713	Phosphorylation	KKKFRTPSFLKKSKK CCCCCCHHHHHHHHH	42.47	19664994
718	Phosphorylation	TPSFLKKSKKKEKVE CHHHHHHHHHHHHCC	48.39	20835925
726	Phosphorylation	KKKEKVES------- HHHHHCCC-------	48.81	9679146

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
55	T	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
55	T	Phosphorylation	Kinase	PKA	-	Uniprot
55	T	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
55	T	Phosphorylation	Kinase	PRKACA	P00517	GPS
489	Y	Phosphorylation	Kinase	FYN	P06241	PSP
592	S	Phosphorylation	Kinase	ERK2	P28482	PSP
596	S	Phosphorylation	Kinase	ERK2	P28482	PSP
600	S	Phosphorylation	Kinase	ERK2	P28482	PSP
604	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
613	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
617	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
693	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
701	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
703	S	Phosphorylation	Kinase	KPCA	P17252	PhosphoELM
703	S	Phosphorylation	Kinase	PRKCA	P05696	GPS
703	S	Phosphorylation	Kinase	PKC	-	Uniprot
713	S	Phosphorylation	Kinase	PKCA	P05696	PSP
713	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
713	S	Phosphorylation	Kinase	PKC	-	Uniprot
713	S	Phosphorylation	Kinase	PKA	-	Uniprot
713	S	Phosphorylation	Kinase	CHEK1	O14757	GPS
713	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
713	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
713	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
713	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
713	S	Phosphorylation	Kinase	PKCA	P04409	PSP
713	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
726	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
726	S	Phosphorylation	Kinase	PKCA	P04409	PSP
726	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
726	S	Phosphorylation	Kinase	PRKCA	P17252	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ADDB_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ADDB_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049
NUD12_HUMAN	NUDT12	physical	28514442
ADDA_HUMAN	ADD1	physical	28514442
ADDG_HUMAN	ADD3	physical	28514442
ROCK1_HUMAN	ROCK1	physical	28514442
ROCK2_HUMAN	ROCK2	physical	28514442
A16A1_HUMAN	ALDH16A1	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ADDB_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-592; SER-596; SER-600;SER-604; SER-693; SER-697 AND SER-699, AND MASS SPECTROMETRY.	19413330 [Abstract]
"Adducin regulation. Definition of the calmodulin-binding domain andsites of phosphorylation by protein kinases A and C."; Matsuoka Y., Hughes C.A., Bennett V.; J. Biol. Chem. 271:25157-25166(1996). Cited for: PHOSPHORYLATION AT THR-55; SER-703 AND SER-713, AND PARTIAL PROTEINSEQUENCE.	8810272 [Abstract]