dbPTM

ADDG_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ADDG_HUMAN
UniProt AC	Q9UEY8
Protein Name	Gamma-adducin
Gene Name	ADD3
Organism	Homo sapiens (Human).
Sequence Length	706
Subcellular Localization	Cytoplasm, cytoskeleton. Cell membrane Peripheral membrane protein Cytoplasmic side.
Protein Description	Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. [PubMed: 23836506 Binds to calmodulin.]
Protein Sequence	MSSDASQGVITTPPPPSMPHKERYFDRINENDPEYIRERNMSPDLRQDFNMMEQRKRVTQILQSPAFREDLECLIQEQMKKGHNPTGLLALQQIADYIMANSFSGFSSPPLSLGMVTPINDLPGADTSSYVKGEKLTRCKLASLYRLVDLFGWAHLANTYISVRISKEQDHIIIIPRGLSFSEATASNLVKVNIIGEVVDQGSTNLKIDHTGFSPHAAIYSTRPDVKCVIHIHTLATAAVSSMKCGILPISQESLLLGDVAYYDYQGSLEEQEERIQLQKVLGPSCKVLVLRNHGVVALGETLEEAFHYIFNVQLACEIQVQALAGAGGVDNLHVLDFQKYKAFTYTVAASGGGGVNMGSHQKWKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTVPLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGGTPIKIEDPNQFVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFEDDDHGPPAPPNPFSHLTEGELEEYKRTIERKQQGLEDAEQELLSDDASSVSQIQSQTQSPQNVPEKLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITIKSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSSDASQGV ------CCCCCCCCC	36.60	20068231
2	Phosphorylation	------MSSDASQGV ------CCCCCCCCC	36.60	28464451
2 (in isoform 2)	Acetylation	-	36.60	-
3	Phosphorylation	-----MSSDASQGVI -----CCCCCCCCCC	35.58	26552605
6	Phosphorylation	--MSSDASQGVITTP --CCCCCCCCCCCCC	32.24	23401153
11	Phosphorylation	DASQGVITTPPPPSM CCCCCCCCCCCCCCC	30.73	23401153
11 (in isoform 2)	Phosphorylation	-	30.73	-
12	Phosphorylation	ASQGVITTPPPPSMP CCCCCCCCCCCCCCC	23.75	23401153
12 (in isoform 2)	Phosphorylation	-	23.75	-
17	Phosphorylation	ITTPPPPSMPHKERY CCCCCCCCCCCHHHH	51.20	23401153
21	Ubiquitination	PPPSMPHKERYFDRI CCCCCCCHHHHHHHC	37.71	-
24	Phosphorylation	SMPHKERYFDRINEN CCCCHHHHHHHCCCC	15.78	26074081
27	Methylation	HKERYFDRINENDPE CHHHHHHHCCCCCHH	24.59	-
35	Phosphorylation	INENDPEYIRERNMS CCCCCHHHHHHCCCC	15.63	55009
42	Phosphorylation	YIRERNMSPDLRQDF HHHHCCCCHHHHHHH	20.86	19664994
42 (in isoform 2)	Phosphorylation	-	20.86	-
59	Phosphorylation	MEQRKRVTQILQSPA HHHHHHHHHHHHCHH	17.58	46159761
64	Phosphorylation	RVTQILQSPAFREDL HHHHHHHCHHHHHHH	18.10	19664994
64 (in isoform 2)	Phosphorylation	-	18.10	-
86	Phosphorylation	MKKGHNPTGLLALQQ HHCCCCHHHHHHHHH	45.86	22210691
97	Phosphorylation	ALQQIADYIMANSFS HHHHHHHHHHHCCCC	5.42	22210691
102	Phosphorylation	ADYIMANSFSGFSSP HHHHHHCCCCCCCCC	15.97	22210691
104	Phosphorylation	YIMANSFSGFSSPPL HHHHCCCCCCCCCCC	38.46	22210691
107	Phosphorylation	ANSFSGFSSPPLSLG HCCCCCCCCCCCEEC	45.52	22210691
108	Phosphorylation	NSFSGFSSPPLSLGM CCCCCCCCCCCEECC	28.42	22210691
112	Phosphorylation	GFSSPPLSLGMVTPI CCCCCCCEECCCCCC	28.75	22210691
117	Phosphorylation	PLSLGMVTPINDLPG CCEECCCCCCCCCCC	15.01	22210691
140	Acetylation	GEKLTRCKLASLYRL CCCCHHHHHHHHHHH	43.74	25953088
159	O-linked_Glycosylation	GWAHLANTYISVRIS HHHHHHCCEEEEEEC	19.34	30379171
162	O-linked_Glycosylation	HLANTYISVRISKEQ HHHCCEEEEEECCCC	8.57	30379171
166	Phosphorylation	TYISVRISKEQDHII CEEEEEECCCCCEEE	21.41	22210691
180	Phosphorylation	IIIPRGLSFSEATAS EEEECCCCCCHHHHH	29.56	20068231
180 (in isoform 2)	Phosphorylation	-	29.56	-
182	Phosphorylation	IPRGLSFSEATASNL EECCCCCCHHHHHCC	23.97	28348404
185	Phosphorylation	GLSFSEATASNLVKV CCCCCHHHHHCCEEE	26.94	20068231
187	Phosphorylation	SFSEATASNLVKVNI CCCHHHHHCCEEEEE	26.75	20068231
187 (in isoform 2)	Phosphorylation	-	26.75	-
203	Phosphorylation	GEVVDQGSTNLKIDH EEEECCCCCCEEECC	14.43	22210691
204	Phosphorylation	EVVDQGSTNLKIDHT EEECCCCCCEEECCC	51.93	22210691
280	Ubiquitination	EERIQLQKVLGPSCK HHHHHHHHHHCCCCE	48.28	-
340	Methylation	LHVLDFQKYKAFTYT EEEECHHHEEEEEEE	49.03	-
351	Phosphorylation	FTYTVAASGGGGVNM EEEEEEECCCCCCCC	28.38	27080861
358	Sulfoxidation	SGGGGVNMGSHQKWK CCCCCCCCCCCCCEE	5.60	30846556
360	Phosphorylation	GGGVNMGSHQKWKVG CCCCCCCCCCCEEEC	16.39	46159743
375	Sulfoxidation	EIEFEGLMRTLDNLG EEEHHHHHHHHHHHC	4.53	30846556
383	Phosphorylation	RTLDNLGYRTGYAYR HHHHHHCCCCCCCCC	14.25	20068231
383 (in isoform 2)	Phosphorylation	-	14.25	-
389	Phosphorylation	GYRTGYAYRHPLIRE CCCCCCCCCCCCCCC	10.85	22817900
390	Methylation	YRTGYAYRHPLIREK CCCCCCCCCCCCCCC	19.44	-
402	Phosphorylation	REKPRHKSDVEIPAT CCCCCCCCCCCCCCE	40.54	30266825
402 (in isoform 2)	Phosphorylation	-	40.54	-
409	Phosphorylation	SDVEIPATVTAFSFE CCCCCCCEEEEEECC	17.03	30266825
411	Phosphorylation	VEIPATVTAFSFEDD CCCCCEEEEEECCCC	20.03	22115753
414	Phosphorylation	PATVTAFSFEDDTVP CCEEEEEECCCCCCC	25.84	21712546
419	Phosphorylation	AFSFEDDTVPLSPLK EEECCCCCCCCCHHH	36.02	21712546
423	Phosphorylation	EDDTVPLSPLKYMAQ CCCCCCCCHHHHHHH	23.32	25159151
423 (in isoform 2)	Phosphorylation	-	23.32	-
426	Ubiquitination	TVPLSPLKYMAQRQQ CCCCCHHHHHHHHHH	36.04	-
427	Phosphorylation	VPLSPLKYMAQRQQR CCCCHHHHHHHHHHH	12.83	26074081
436	Acetylation	AQRQQREKTRWLNSP HHHHHHHHHHHCCCC	45.65	7427887
442	Phosphorylation	EKTRWLNSPNTYMKV HHHHHCCCCCCEEEE	19.84	25159151
442 (in isoform 2)	Phosphorylation	-	19.84	-
445	Phosphorylation	RWLNSPNTYMKVNVP HHCCCCCCEEEEECC	28.51	22115753
446	Phosphorylation	WLNSPNTYMKVNVPE HCCCCCCEEEEECCH	11.11	22617229
448	Ubiquitination	NSPNTYMKVNVPEES CCCCCEEEEECCHHH	21.90	21890473
448	Ubiquitination	NSPNTYMKVNVPEES CCCCCEEEEECCHHH	21.90	21890473
448 (in isoform 1)	Ubiquitination	-	21.90	21890473
448 (in isoform 2)	Ubiquitination	-	21.90	21890473
455	Phosphorylation	KVNVPEESRNGETSP EEECCHHHCCCCCCC	29.41	25159151
460	Phosphorylation	EESRNGETSPRTKIT HHHCCCCCCCCCEEE	45.49	25159151
461	Phosphorylation	ESRNGETSPRTKITW HHCCCCCCCCCEEEE	14.21	28355574
461 (in isoform 2)	Phosphorylation	-	14.21	-
464	O-linked_Glycosylation	NGETSPRTKITWMKA CCCCCCCCEEEEEEE	30.13	30379171
464	Phosphorylation	NGETSPRTKITWMKA CCCCCCCCEEEEEEE	30.13	30576142
467	Phosphorylation	TSPRTKITWMKAEDS CCCCCEEEEEEECCC	22.51	46159755
474	Phosphorylation	TWMKAEDSSKVSGGT EEEEECCCCCCCCCC	24.46	28857561
478	Phosphorylation	AEDSSKVSGGTPIKI ECCCCCCCCCCCCCC	34.93	23401153
478 (in isoform 2)	Phosphorylation	-	34.93	-
481	Phosphorylation	SSKVSGGTPIKIEDP CCCCCCCCCCCCCCC	25.93	25159151
484	Sumoylation	VSGGTPIKIEDPNQF CCCCCCCCCCCCCCC	41.04	28112733
504	Ubiquitination	NPNEVLEKRNKIREQ CHHHHHHHHHHHHHH	58.05	-
514	Phosphorylation	KIREQNRYDLKTAGP HHHHHHCCCCCCCCC	32.93	28796482
565	Phosphorylation	TEGELEEYKRTIERK CHHHHHHHHHHHHHH	9.08	46159773
585	Phosphorylation	DAEQELLSDDASSVS HHHHHHHCCCHHHHH	46.17	28464451
589	Phosphorylation	ELLSDDASSVSQIQS HHHCCCHHHHHHHHH	37.96	26657352
590	Phosphorylation	LLSDDASSVSQIQSQ HHCCCHHHHHHHHHH	27.75	30576142
592	Phosphorylation	SDDASSVSQIQSQTQ CCCHHHHHHHHHHCC	24.42	26074081
596	Phosphorylation	SSVSQIQSQTQSPQN HHHHHHHHHCCCCCC	36.50	29496963
598	Phosphorylation	VSQIQSQTQSPQNVP HHHHHHHCCCCCCCC	35.94	28464451
600	Phosphorylation	QIQSQTQSPQNVPEK HHHHHCCCCCCCCHH	31.66	26657352
616	Phosphorylation	EENHELFSKSFISME HHHHHHHHHHCEECC	39.76	24719451
618	Phosphorylation	NHELFSKSFISMEVP HHHHHHHHCEECCCC	26.98	22199227
618 (in isoform 2)	Phosphorylation	-	26.98	-
621	Phosphorylation	LFSKSFISMEVPVMV HHHHHCEECCCCEEE	13.59	29802988
641 (in isoform 2)	Phosphorylation	-	36.80	-
645 (in isoform 2)	Phosphorylation	-	26.49	-
647	Phosphorylation	DELAKRVSRLSTSTT HHHHHHHHHHCCCCE	31.38	22617229
647 (in isoform 2)	Phosphorylation	-	31.38	-
649 (in isoform 2)	Phosphorylation	-	5.22	-
650	Phosphorylation	AKRVSRLSTSTTIEN HHHHHHHCCCCEEEE	21.14	25159151
651	Phosphorylation	KRVSRLSTSTTIENI HHHHHHCCCCEEEEE	34.38	25159151
651 (in isoform 2)	Phosphorylation	-	34.38	-
652	Phosphorylation	RVSRLSTSTTIENIE HHHHHCCCCEEEEEE	21.85	24706070
653	Phosphorylation	VSRLSTSTTIENIEI HHHHCCCCEEEEEEE	31.70	28122231
654	Phosphorylation	SRLSTSTTIENIEIT HHHCCCCEEEEEEEE	26.51	28348404
659 (in isoform 2)	Phosphorylation	-	45.74	-
661	Phosphorylation	TIENIEITIKSPEKI EEEEEEEEECCHHHH	15.01	22199227
661 (in isoform 2)	Phosphorylation	-	15.01	-
664	Phosphorylation	NIEITIKSPEKIEEV EEEEEECCHHHHHHH	34.15	23927012
673	Phosphorylation	EKIEEVLSPEGSPSK HHHHHHHCCCCCCCC	26.53	19664994
677	Phosphorylation	EVLSPEGSPSKSPSK HHHCCCCCCCCCCCH	25.08	19664994
679	Phosphorylation	LSPEGSPSKSPSKKK HCCCCCCCCCCCHHC	47.68	29255136
681	Phosphorylation	PEGSPSKSPSKKKKK CCCCCCCCCCHHCCC	38.45	19664994
683	Phosphorylation	GSPSKSPSKKKKKFR CCCCCCCCHHCCCCC	65.29	29255136
691	Phosphorylation	KKKKKFRTPSFLKKN HHCCCCCCHHHHHHC	26.81	30266825
693	Phosphorylation	KKKFRTPSFLKKNKK CCCCCCHHHHHHCCH	42.47	19664994

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
423	S	Phosphorylation	Kinase	CDK2	P24941	PSP
693	S	Phosphorylation	Kinase	CHEK1	O14757	GPS
693	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
693	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ADDG_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ADDG_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of ADDG_HUMAN !!

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ADDG_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402; SER-423; SER-673;SER-677 AND SER-681, AND MASS SPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-423; SER-442;SER-673; SER-677; SER-681 AND SER-693, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Phosphoproteome of resting human platelets."; Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,Schuetz C., Walter U., Gambaryan S., Sickmann A.; J. Proteome Res. 7:526-534(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-681, AND MASSSPECTROMETRY.	18088087 [Abstract]
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."; Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.; Anal. Sci. 24:161-166(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-681, AND MASSSPECTROMETRY.	18187866 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-673; SER-677 ANDSER-693, AND MASS SPECTROMETRY.	17081983 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-12, AND MASSSPECTROMETRY.	19413330 [Abstract]