TRUA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRUA_HUMAN
• UniProt AC: Q9Y606
• Protein Name: tRNA pseudouridine synthase A
• Gene Name: PUS1
• Organism: Homo sapiens (Human).
• Sequence Length: 427
• Subcellular Localization: Isoform 1: Mitochondrion .; Isoform 2: Nucleus .
• Protein Description: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 RNA..
• Protein Sequence: MGLQLRALLGAFGRWTLRLGPRPSCSPRMAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
26	Phosphorylation	LGPRPSCSPRMAGNA CCCCCCCCCCCCCCC	21.63	28555341
67	Acetylation	EDGEHPAKKLKSGGD CCCCCCHHHCCCCCC	64.19	25953088
110	Ubiquitination	NVGSSQFKTIEDDLV CCCCHHCCCHHHHHH	40.74	-
138	Phosphorylation	GEDMRKMSFQRCART CHHHHHHHHHHHHCC	22.66	24719451
145	Phosphorylation	SFQRCARTDKGVSAA HHHHHHCCCCCCCCC	24.44	29759185
147	Acetylation	QRCARTDKGVSAAGQ HHHHCCCCCCCCCCC	61.67	25953088
147	Malonylation	QRCARTDKGVSAAGQ HHHHCCCCCCCCCCC	61.67	26320211
150	Phosphorylation	ARTDKGVSAAGQVVS HCCCCCCCCCCCEEE	22.52	-
176	Phosphorylation	KINSHLPSHIRILGL HHHHCCCCCEEEECC	37.60	-
187	Phosphorylation	ILGLKRVTGGFNSKN EECCCCCCCCCCCCC	35.30	25599653
201	Phosphorylation	NRCDARTYCYLLPTF CCCCHHHHHHHHHHH	3.58	22210691
207	Phosphorylation	TYCYLLPTFAFAHKD HHHHHHHHHHHHCCC	27.80	22210691
221	Phosphorylation	DRDVQDETYRLSAET CCCCCCCHHCCCHHH	23.41	20068231
222	Phosphorylation	RDVQDETYRLSAETL CCCCCCHHCCCHHHH	13.91	22210691
239	Phosphorylation	VNRLLACYKGTHNFH HHHHHHHHCCCCCCC	13.57	22817900
249	Phosphorylation	THNFHNFTSQKGPQD CCCCCCCCCCCCCCC	35.25	28857561
250	Phosphorylation	HNFHNFTSQKGPQDP CCCCCCCCCCCCCCH	26.25	20873877
342	Acetylation	LERVHFEKYNQRFGN EEEEEHHHHHHHCCC	48.86	26822725
364	Ubiquitination	DWAQEEGKVAAFKEE HHHHHHCCEEEEEHH	31.79	-
369	Ubiquitination	EGKVAAFKEEHIYPT HCCEEEEEHHHCCCE	58.42	-
369	2-Hydroxyisobutyrylation	EGKVAAFKEEHIYPT HCCEEEEEHHHCCCE	58.42	-
380	Phosphorylation	IYPTIIGTERDERSM CCCEECCCCCCHHHH	19.89	24719451
392	Phosphorylation	RSMAQWLSTLPIHNF HHHHHHHHCCCCCCC	25.57	19664995
415	Phosphorylation	GTGAKVPSPLEGSEG CCCCCCCCCCCCCCC	45.14	30266825
420	Phosphorylation	VPSPLEGSEGDGDTD CCCCCCCCCCCCCCC	29.09	23927012
426	Phosphorylation	GSEGDGDTD------ CCCCCCCCC------	49.49	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TRUA_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TRUA_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRUA_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ARP3_HUMAN	ACTR3	physical	22863883
GDE_HUMAN	AGL	physical	22863883
ARPC2_HUMAN	ARPC2	physical	22863883
BCAT1_HUMAN	BCAT1	physical	22863883
SYFA_HUMAN	FARSA	physical	22863883
IDE_HUMAN	IDE	physical	22863883
NCBP1_HUMAN	NCBP1	physical	22863883
NRDC_HUMAN	NRD1	physical	22863883
NSUN2_HUMAN	NSUN2	physical	22863883
PFD1_HUMAN	PFDN1	physical	22863883
PFD5_HUMAN	PFDN5	physical	22863883
RFA1_HUMAN	RPA1	physical	22863883
SP100_HUMAN	SP100	physical	22863883
VPS29_HUMAN	VPS29	physical	22863883

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 600462: Myopathy with lactic acidosis and sideroblastic anemia 1 (MLASA1)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-415; SER-420 ANDTHR-426, AND MASS SPECTROMETRY.
PubMed: 19413330

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-420 AND THR-426, ANDMASS SPECTROMETRY.
PubMed: 18669648

"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-420 AND THR-426, ANDMASS SPECTROMETRY.
PubMed: 17081983

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-426, AND MASSSPECTROMETRY.
PubMed: 19690332

"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization.";
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
Nat. Biotechnol. 24:1285-1292(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-426, AND MASSSPECTROMETRY.
PubMed: 16964243

"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer.";
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.;
Cell 131:1190-1203(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, AND MASSSPECTROMETRY.
PubMed: 18083107