dbPTM

TOP2B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TOP2B_MOUSE
UniProt AC	Q64511
Protein Name	DNA topoisomerase 2-beta
Gene Name	Top2b
Organism	Mus musculus (Mouse).
Sequence Length	1612
Subcellular Localization	Nucleus, nucleolus.
Protein Description	Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks..
Protein Sequence	MAKSSLAGSDGALTWVNNATKKEELETANKNDSTKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEITAMKADASRKLLKKKKGDPDTTVVKVEFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSTGKTNAKKVKKRNPWSDDESKSESDLEEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDAAAADDSNDLEELKVKASPITNDGEDEFVPSDGLDKDEYAFSSGKSKATPEKSSNDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDTASVFAPSFGLKQTDKLPSKTVAAKKGKPPSDTAPKAKRAPKQKKIVETINSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKPSKTASKKPKKTSFDQDSDVDIFPSDFTSEPPALPRTGRARKEVKYFAESDEEEDVDFAMFN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAKSSLAGS ------CCCCCCCCC	31.57	23806337
3	Acetylation	-----MAKSSLAGSD -----CCCCCCCCCC	53.06	23806337
4	Phosphorylation	----MAKSSLAGSDG ----CCCCCCCCCCC	37.33	26643407
5	Phosphorylation	---MAKSSLAGSDGA ---CCCCCCCCCCCC	22.87	26643407
9	Phosphorylation	AKSSLAGSDGALTWV CCCCCCCCCCCHHHC	27.54	26643407
14	Phosphorylation	AGSDGALTWVNNATK CCCCCCHHHCCCCCC	27.17	22802335
20	Phosphorylation	LTWVNNATKKEELET HHHCCCCCCHHHHHH	44.78	22802335
21	Acetylation	TWVNNATKKEELETA HHCCCCCCHHHHHHC	56.64	22826441
263	Malonylation	AGSCKGVKVMFNGKK HCCCCEEEEEECCEE	36.33	26320211
414	S-nitrosylation	FFKAASNCGIVESIL HHHHHHCCCHHHHHH	3.49	20925432
414	S-nitrosocysteine	FFKAASNCGIVESIL HHHHHHCCCHHHHHH	3.49	-
419	Phosphorylation	SNCGIVESILNWVKF HCCCHHHHHHHHHHH	22.83	17203969
529	Malonylation	IVGLQYKKSYDDAES HHCEEECCCCCCHHH	49.68	26320211
538	Acetylation	YDDAESLKTLRYGKI CCCHHHHHHCCCCEE	55.59	22826441
627	Phosphorylation	KYYKGLGTSTAKEAK EEECCCCCCCHHHHH	28.13	17203969
716	Phosphorylation	NKELILFSNSDNERS CCEEEEEECCCCCCC	31.79	28066266
718	Phosphorylation	ELILFSNSDNERSIP EEEEEECCCCCCCCC	40.38	28066266
764	Phosphorylation	AGSVAEMSAYHHGEQ HHHHHHHHHCHHHHH	19.72	22802335
766	Phosphorylation	SVAEMSAYHHGEQAL HHHHHHHCHHHHHHH	6.24	22802335
776	Phosphorylation	GEQALMMTIVNLAQN HHHHHHHHHHHHHHH	14.06	22802335
787	Phosphorylation	LAQNFVGSNNINLLQ HHHHCCCCCCCCEEE	22.77	22802335
969	Phosphorylation	LISDYKEYHTDTTVK HHHCHHHHCCCCEEE	13.08	28576409
1039	Phosphorylation	FDLRLSYYGLRKEWL HHHHHHHHHCCHHHH	13.30	29109428
1120	Phosphorylation	QNQHDDSSSDSGTPS CCCCCCCCCCCCCCC	45.20	29899451
1121	Phosphorylation	NQHDDSSSDSGTPSG CCCCCCCCCCCCCCC	39.48	29899451
1123	Phosphorylation	HDDSSSDSGTPSGPD CCCCCCCCCCCCCCC	46.65	29899451
1125	Phosphorylation	DSSSDSGTPSGPDFN CCCCCCCCCCCCCHH	20.45	29899451
1127	Phosphorylation	SSDSGTPSGPDFNYI CCCCCCCCCCCHHHH	63.90	27600695
1133	Phosphorylation	PSGPDFNYILNMSLW CCCCCHHHHHHHHHH	13.53	27600695
1168	Phosphorylation	VNDLKRKSPSDLWKE HHHHHHCCHHHHHHH	33.40	28066266
1170	Phosphorylation	DLKRKSPSDLWKEDL HHHHCCHHHHHHHHH	53.04	28066266
1200	Phosphorylation	EDILAGMSGKAIKGK HHHHHHCCCCHHCCC	35.90	28066266
1202	Acetylation	ILAGMSGKAIKGKVG HHHHCCCCHHCCCCC	39.36	23806337
1221	Phosphorylation	KKLQLEETMPSPYGR CEEECEECCCCCCCC	25.63	26643407
1224	Phosphorylation	QLEETMPSPYGRRIV ECEECCCCCCCCCCH	21.67	26745281
1226	Phosphorylation	EETMPSPYGRRIVPE EECCCCCCCCCCHHH	27.83	26745281
1267	Phosphorylation	VEFDEEFSGTPAEGT EEECCCCCCCCCCCC	45.07	25619855
1269	Phosphorylation	FDEEFSGTPAEGTGE ECCCCCCCCCCCCCC	20.37	25619855
1274	Phosphorylation	SGTPAEGTGEETLTP CCCCCCCCCCCCCCC	32.69	25293948
1278	Phosphorylation	AEGTGEETLTPSAPV CCCCCCCCCCCCCCC	31.46	25293948
1280	Phosphorylation	GTGEETLTPSAPVNK CCCCCCCCCCCCCCC	23.71	26824392
1282	Phosphorylation	GEETLTPSAPVNKGP CCCCCCCCCCCCCCC	39.71	25293948
1300	Phosphorylation	REKKEPGTRVRKTPT CCCCCCCCCCCCCCC	36.38	20531401
1304	Acetylation	EPGTRVRKTPTSTGK CCCCCCCCCCCCCCC	56.87	19852639
1305	Phosphorylation	PGTRVRKTPTSTGKT CCCCCCCCCCCCCCC	22.64	21183079
1308	Phosphorylation	RVRKTPTSTGKTNAK CCCCCCCCCCCCCCC	36.04	21183079
1311	Acetylation	KTPTSTGKTNAKKVK CCCCCCCCCCCCHHH	38.40	23806337
1315	Acetylation	STGKTNAKKVKKRNP CCCCCCCCHHHHCCC	61.23	19852651
1316	Acetylation	TGKTNAKKVKKRNPW CCCCCCCHHHHCCCC	57.80	19852663
1324	Phosphorylation	VKKRNPWSDDESKSE HHHCCCCCCCCCCCH	36.12	25521595
1328	Phosphorylation	NPWSDDESKSESDLE CCCCCCCCCCHHHHH	48.77	25521595
1330	Phosphorylation	WSDDESKSESDLEEA CCCCCCCCHHHHHHC	52.17	21082442
1332	Phosphorylation	DDESKSESDLEEAEP CCCCCCHHHHHHCCC	55.37	25521595
1346	Phosphorylation	PVVIPRDSLLRRAAA CEEECHHHHHHHHHH	30.50	-
1358	Phosphorylation	AAAERPKYTFDFSEE HHHHCCCEEECCCHH	18.47	24925903
1359	Phosphorylation	AAERPKYTFDFSEEE HHHCCCEEECCCHHC	23.89	24925903
1363	Phosphorylation	PKYTFDFSEEEDDDA CCEEECCCHHCCCCH	46.04	24925903
1376	Phosphorylation	DAAAADDSNDLEELK CHHCCCCCCCHHHHC	32.56	24925903
1387	Phosphorylation	EELKVKASPITNDGE HHHCCCEECCCCCCC	15.89	27087446
1390	Phosphorylation	KVKASPITNDGEDEF CCCEECCCCCCCCCC	30.55	25521595
1400	Phosphorylation	GEDEFVPSDGLDKDE CCCCCCCCCCCCCCH	38.72	27087446
1408	Phosphorylation	DGLDKDEYAFSSGKS CCCCCCHHHHCCCCC	24.07	21082442
1411	Phosphorylation	DKDEYAFSSGKSKAT CCCHHHHCCCCCCCC	29.97	23375375
1412	Phosphorylation	KDEYAFSSGKSKATP CCHHHHCCCCCCCCC	43.73	21082442
1422	Phosphorylation	SKATPEKSSNDKKSQ CCCCCCCCCCCCCCC	32.48	26745281
1423	Phosphorylation	KATPEKSSNDKKSQD CCCCCCCCCCCCCCC	61.18	26745281
1428	Phosphorylation	KSSNDKKSQDFGNLF CCCCCCCCCCCHHCC	40.50	28833060
1436	Phosphorylation	QDFGNLFSFPSYSQK CCCHHCCCCCCCCCC	39.14	28833060
1439	Phosphorylation	GNLFSFPSYSQKSED HHCCCCCCCCCCCCC	35.09	21082442
1440	Phosphorylation	NLFSFPSYSQKSEDD HCCCCCCCCCCCCCC	18.50	28833060
1441	Phosphorylation	LFSFPSYSQKSEDDS CCCCCCCCCCCCCCC	35.15	28833060
1444	Phosphorylation	FPSYSQKSEDDSAKF CCCCCCCCCCCCCCC	38.32	27087446
1448	Phosphorylation	SQKSEDDSAKFDSNE CCCCCCCCCCCCCCH	45.76	22942356
1453	Phosphorylation	DDSAKFDSNEEDTAS CCCCCCCCCHHHHHH	49.64	27087446
1458	Phosphorylation	FDSNEEDTASVFAPS CCCCHHHHHHHCCHH	24.90	17203969
1460	Phosphorylation	SNEEDTASVFAPSFG CCHHHHHHHCCHHHC	21.62	27087446
1465	Phosphorylation	TASVFAPSFGLKQTD HHHHCCHHHCCCCCC	28.63	25619855
1506	Phosphorylation	KQKKIVETINSDSDS CHHHHEEECCCCCCC	17.98	24925903
1509	Phosphorylation	KIVETINSDSDSEFG HHEEECCCCCCCCCC	34.34	27087446
1511	Phosphorylation	VETINSDSDSEFGIP EEECCCCCCCCCCCC	42.74	27087446
1513	Phosphorylation	TINSDSDSEFGIPKK ECCCCCCCCCCCCCC	38.48	24925903
1521	Phosphorylation	EFGIPKKTTTPKGKG CCCCCCCCCCCCCCC	41.87	24759943
1522	Phosphorylation	FGIPKKTTTPKGKGR CCCCCCCCCCCCCCC	50.40	24759943
1523	Phosphorylation	GIPKKTTTPKGKGRG CCCCCCCCCCCCCCC	28.38	25367039
1535	Acetylation	GRGAKKRKASGSENE CCCCCCCCCCCCCCC	56.64	7627767
1537	Phosphorylation	GAKKRKASGSENEGD CCCCCCCCCCCCCCC	45.98	27087446
1539	Phosphorylation	KKRKASGSENEGDYN CCCCCCCCCCCCCCC	34.45	27087446
1545	Phosphorylation	GSENEGDYNPGRKPS CCCCCCCCCCCCCCC	33.83	28833060
1552	Phosphorylation	YNPGRKPSKTASKKP CCCCCCCCCCCCCCC	45.75	26745281
1553	Acetylation	NPGRKPSKTASKKPK CCCCCCCCCCCCCCC	57.55	19855909
1557	Acetylation	KPSKTASKKPKKTSF CCCCCCCCCCCCCCC	71.30	19855921
1558	Acetylation	PSKTASKKPKKTSFD CCCCCCCCCCCCCCC	60.62	19855933
1562	Phosphorylation	ASKKPKKTSFDQDSD CCCCCCCCCCCCCCC	40.53	27087446
1563	Phosphorylation	SKKPKKTSFDQDSDV CCCCCCCCCCCCCCC	35.39	25521595
1568	Phosphorylation	KTSFDQDSDVDIFPS CCCCCCCCCCCCCCC	33.62	27087446
1575	Phosphorylation	SDVDIFPSDFTSEPP CCCCCCCCCCCCCCC	34.31	24925903
1578	Phosphorylation	DIFPSDFTSEPPALP CCCCCCCCCCCCCCC	36.85	25619855
1579	Phosphorylation	IFPSDFTSEPPALPR CCCCCCCCCCCCCCC	48.36	25619855
1596	Phosphorylation	RARKEVKYFAESDEE CCHHHCEEHHCCCCC	17.13	25619855
1600	Phosphorylation	EVKYFAESDEEEDVD HCEEHHCCCCCCCCC	47.06	24925903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of TOP2B_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of TOP2B_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TOP2B_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ZN451_HUMAN	ZNF451	physical	20360068
TOP2A_HUMAN	TOP2A	physical	20360068
ITPR1_HUMAN	ITPR1	physical	20360068
TOP2B_HUMAN	TOP2B	physical	20360068

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TOP2B_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry."; Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.; Mol. Cell. Proteomics 8:904-912(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1453; SER-1509 ANDSER-1511, AND MASS SPECTROMETRY.	19131326 [Abstract]
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1509, AND MASSSPECTROMETRY.	19144319 [Abstract]
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1324; SER-1328;SER-1330; SER-1332; SER-1387; SER-1509; SER-1511; SER-1537 ANDSER-1539, AND MASS SPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1387; SER-1453;SER-1509; SER-1511; SER-1513; SER-1537 AND SER-1539, AND MASSSPECTROMETRY.	17242355 [Abstract]
"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry."; Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; J. Proteome Res. 6:250-262(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419; THR-627; SER-1387;SER-1400; SER-1453 AND THR-1458, AND MASS SPECTROMETRY.	17203969 [Abstract]