TF2H2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TF2H2_HUMAN
• UniProt AC: Q13888
• Protein Name: General transcription factor IIH subunit 2
• Gene Name: GTF2H2
• Organism: Homo sapiens (Human).
• Sequence Length: 395
• Subcellular Localization: Nucleus .
• Protein Description: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter..
• Protein Sequence: MDEEPERTKRWEGGYERTWEILKEDESGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRHLYVVVDGSRTMEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITSLKKAVDMTCHGEPSLYNSLSIAMQTLKHMPGHTSREVLIIFSSLTTCDPSNIYDLIKTLKAAKIRVSVIGLSAEVRVCTVLARETGGTYHVILDESHYKELLTHHVSPPPASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDGNTEPGLTLGGYFCPQCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEIPLEEYNGERFCYGCQGELKDQHVYVCAVCQNVFCVDCDVFVHDSLHCCPGCIHKIPAPSGV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Phosphorylation	TKRWEGGYERTWEIL HHCCCCCHHHHHHHH	17.07	-
23	Ubiquitination	ERTWEILKEDESGSL HHHHHHHEECCCCCC	69.64	21906983
31	Ubiquitination	EDESGSLKATIEDIL ECCCCCCEEEHHHHH	45.22	32015554
40	Ubiquitination	TIEDILFKAKRKRVF EHHHHHHHHHHHHHH	49.94	21906983
40	Methylation	TIEDILFKAKRKRVF EHHHHHHHHHHHHHH	49.94	-
42	Ubiquitination	EDILFKAKRKRVFEH HHHHHHHHHHHHHHH	60.07	22817900
44	Ubiquitination	ILFKAKRKRVFEHHG HHHHHHHHHHHHHHC	52.81	22817900
62	Phosphorylation	LGMMRHLYVVVDGSR EECCEEEEEEEECCC	5.76	29496907
70	Phosphorylation	VVVDGSRTMEDQDLK EEEECCCCCCCCCCC	26.98	22210691
77	Ubiquitination	TMEDQDLKPNRLTCT CCCCCCCCCCHHHHH	48.84	21906983
90	Phosphorylation	CTLKLLEYFVEEYFD HHHHHHHHHHHHHCC	16.94	-
95	Phosphorylation	LEYFVEEYFDQNPIS HHHHHHHHCCCCCCH	10.03	-
112	Ubiquitination	GIIVTKSKRAEKLTE EEEEECCHHHHHHHH	58.12	22817900
116	Ubiquitination	TKSKRAEKLTELSGN ECCHHHHHHHHHCCC	60.78	22817900
126	Ubiquitination	ELSGNPRKHITSLKK HHCCCCHHHHHHHHH	40.39	-
132	Ubiquitination	RKHITSLKKAVDMTC HHHHHHHHHHHHCCC	38.15	29967540
133	Ubiquitination	KHITSLKKAVDMTCH HHHHHHHHHHHCCCC	59.96	29967540
202	Phosphorylation	RVSVIGLSAEVRVCT EEEEEECCEEEEEEE	19.71	20068231
233	Phosphorylation	SHYKELLTHHVSPPP HHHHHHHHCCCCCCC	23.66	24114839
237	Phosphorylation	ELLTHHVSPPPASSS HHHHCCCCCCCCCCC	27.60	25159151
244	Phosphorylation	SPPPASSSSECSLIR CCCCCCCCCCCCEEE	27.88	24114839
245	Phosphorylation	PPPASSSSECSLIRM CCCCCCCCCCCEEEC	44.57	24114839
248	Phosphorylation	ASSSSECSLIRMGFP CCCCCCCCEEECCCC	23.84	24114839
297	Ubiquitination	FCPQCRAKYCELPVE ECHHHCCCCCCCCCE	30.95	33845483
306	Ubiquitination	CELPVECKICGLTLV CCCCCEEEECCCEEE	27.76	21963094

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TF2H2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TF2H2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TF2H2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TF2H5_HUMAN	GTF2H5	physical	15220921
ERCC2_HUMAN	ERCC2	physical	11062469
TF2H4_HUMAN	GTF2H4	physical	11062469
CDK7_HUMAN	CDK7	physical	11062469
CCNH_HUMAN	CCNH	physical	11062469
TF2H5_HUMAN	GTF2H5	physical	11062469
TF2H1_HUMAN	GTF2H1	physical	11062469
ERCC2_HUMAN	ERCC2	physical	12820975
ERCC8_HUMAN	ERCC8	physical	7664335
TF2H1_HUMAN	GTF2H1	physical	8194529
ERCC3_HUMAN	ERCC3	physical	8194529
TF2H4_HUMAN	GTF2H4	physical	22939629
TF2H3_HUMAN	GTF2H3	physical	22939629
SRA1_HUMAN	SRA1	physical	20398657
TF2H5_HUMAN	GTF2H5	physical	16669699
TF2H4_HUMAN	GTF2H4	physical	26344197
TF2H3_HUMAN	GTF2H3	physical	26340423
ERCC2_HUMAN	ERCC2	physical	26340423

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.