TBX21_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TBX21_HUMAN
• UniProt AC: Q9UL17
• Protein Name: T-box transcription factor TBX21
• Gene Name: TBX21
• Organism: Homo sapiens (Human).
• Sequence Length: 535
• Subcellular Localization: Nucleus .
• Protein Description: Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. [PubMed: 10761931 Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells]
• Protein Sequence: MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSRFLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVALNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENFESMYTSVDTSIPSPPGPNCQFLGGDHYSPLLPNQYPVPSRFYPDLPGQAKDVVPQAYWLGAPRDHSYEAEFRAVSMKPAFLPSAPGPTMSYYRGQEVLAPGAGWPVAPQYPPKMGPASWFRPMRTLPMEPGPGGSEGRGPEDQGPPLVWTEIAPIRPESSDSGLGEGDSKRRRVSPYPSSGDSSSPAGAPSPFDKEAEGQFYNYFPN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
53	Phosphorylation	DERRGGGSLGSPYPG CCCCCCCCCCCCCCC	32.60	-
58	Phosphorylation	GGSLGSPYPGGALVP CCCCCCCCCCCCEEC	19.06	22817900
77	Phosphorylation	RFLGAYAYPPRPQAA CCCCCEECCCCCCCC	10.90	-
118	Phosphorylation	PDPRAGLYPGPREDY CCCCCCCCCCCHHHC	12.77	29978859
125	Phosphorylation	YPGPREDYALPAGLE CCCCHHHCCCCCCCE	12.80	29978859
161	Ubiquitination	QTEMIITKQGRRMFP HCEEEEECCCCCEEC	40.11	-
220	Phosphorylation	SMPGNRLYVHPDSPN CCCCCEEEECCCCCC	8.09	21690296
225	Phosphorylation	RLYVHPDSPNTGAHW EEEECCCCCCCCCCC	25.22	-
251	Phosphorylation	LTNNKGASNNVTQMI ECCCCCCCCCEEEEE	37.29	-
255	Phosphorylation	KGASNNVTQMIVLQS CCCCCCEEEEEHHHH	17.97	-
262	Phosphorylation	TQMIVLQSLHKYQPR EEEEHHHHHHHCCCC	28.42	-
266	Phosphorylation	VLQSLHKYQPRLHIV HHHHHHHCCCCEEEE	17.01	21690296
303	Phosphorylation	ETQFIAVTAYQNAEI CCEEEEEEEECCCEE	15.60	-
305	Phosphorylation	QFIAVTAYQNAEITQ EEEEEEEECCCEEEE	7.92	21690296
314	Ubiquitination	NAEITQLKIDNNPFA CCEEEEEEECCCHHH	37.89	-
322	Ubiquitination	IDNNPFAKGFRENFE ECCCHHHHHHHHHHH	59.99	-
394	Phosphorylation	LGAPRDHSYEAEFRA CCCCCCCCHHHEEEE	29.02	30108239
395	Phosphorylation	GAPRDHSYEAEFRAV CCCCCCCHHHEEEEE	18.75	30108239
403	Phosphorylation	EAEFRAVSMKPAFLP HHEEEEEECCCCCCC	21.80	22210691
411	Phosphorylation	MKPAFLPSAPGPTMS CCCCCCCCCCCCCEE	49.60	22210691
421	Methylation	GPTMSYYRGQEVLAP CCCEEEECCCEEECC	31.89	115389257
438	Phosphorylation	GWPVAPQYPPKMGPA CCCCCCCCCCCCCCC	22.05	22210691
503	Phosphorylation	DSKRRRVSPYPSSGD CCCCCCCCCCCCCCC	19.53	23312004
505	Phosphorylation	KRRRVSPYPSSGDSS CCCCCCCCCCCCCCC	14.29	30301811
507	Phosphorylation	RRVSPYPSSGDSSSP CCCCCCCCCCCCCCC	40.90	30301811
508	Phosphorylation	RVSPYPSSGDSSSPA CCCCCCCCCCCCCCC	41.96	30301811
511	Phosphorylation	PYPSSGDSSSPAGAP CCCCCCCCCCCCCCC	35.92	30301811
512	Phosphorylation	YPSSGDSSSPAGAPS CCCCCCCCCCCCCCC	45.11	23312004
513	Phosphorylation	PSSGDSSSPAGAPSP CCCCCCCCCCCCCCC	23.79	30301811
519	Phosphorylation	SSPAGAPSPFDKEAE CCCCCCCCCCCHHHC	37.35	28348404
530	Phosphorylation	KEAEGQFYNYFPN-- HHHCCCCCCCCCC--	10.89	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
220	Y	Phosphorylation	Kinase	ABL	P00519	PSP
266	Y	Phosphorylation	Kinase	ABL	P00519	PSP
305	Y	Phosphorylation	Kinase	ABL	P00519	PSP
503	S	Phosphorylation	Kinase	RPS6KA3	P51812	GPS
507	S	Phosphorylation	Kinase	RPS6KA3	P51812	GPS
530	Y	Phosphorylation	Kinase	ITK	Q08881	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
53	S	Phosphorylation	Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1.	-
225	S	Phosphorylation	Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1.	-
303	T	Phosphorylation	Phosphorylation at Thr-303 is required for its interaction with NFATC2.	-
303	T	Phosphorylation	Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-303 (By similarity).	24845384
303	T	ubiquitylation	Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-303 (By similarity).	24845384
314	K	ubiquitylation	Ubiquitinated at Lys-314, leading to its degradation by the proteasome.	24845384
513	S	Phosphorylation	Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TBX21_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CBP_HUMAN	CREBBP	physical	17075044
EP300_HUMAN	EP300	physical	17075044
GATA3_HUMAN	GATA3	physical	17075044
SP1_HUMAN	SP1	physical	17075044
ZN490_HUMAN	ZNF490	physical	20211142
UBP10_HUMAN	USP10	physical	24845384
UBC_HUMAN	UBC	physical	24845384

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 208550: Asthma, with nasal polyps and aspirin intolerance (ANPAI)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.