dbPTM

SMAD6_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SMAD6_MOUSE
UniProt AC	O35182
Protein Name	Mothers against decapentaplegic homolog 6
Gene Name	Smad6
Organism	Mus musculus (Mouse).
Sequence Length	495
Subcellular Localization	Nucleus.
Protein Description	Binds to regulatory elements in target promoter regions (By similarity). May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding (By similarity). Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes..
Protein Sequence	MFRSKRSGLVRRLWRSRVVPDREEGSGGGGGVDEDGSLGSRAEPAPRAREGGGCSRSEVRSVAPRRPRDAVGPRGAAIAGRRRRTGGLPRPVSESGAGAGGSPLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPRDSGDPQARQSPEPEEGGGPRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVLVPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFTAAADGPTVCCNPYHFSRLCGPESPPPPYSRLSPPDQYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLLQHADAAHGPYDPHSVRISFAKGWGPCYSRQFITSCPCWLEILLNNHR

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
57	Phosphorylation	EGGGCSRSEVRSVAP CCCCCCHHHHHHCCC	24.93	28059163
74	Dimethylation	PRDAVGPRGAAIAGR CCCCCCCCCHHHCCC	41.26	-
74	Methylation	PRDAVGPRGAAIAGR CCCCCCCCCHHHCCC	41.26	23747011
81	Methylation	RGAAIAGRRRRTGGL CCHHHCCCCCCCCCC	21.65	23747011
148	Phosphorylation	GDPQARQSPEPEEGG CCCCHHCCCCCCCCC	26.19	27087446
175	Phosphorylation	LLEQELKTVTYSLLK HHHHHHHHHHHHHHH	30.61	22817900
188	Phosphorylation	LKRLKERSLDTLLEA HHHHHHCCHHHHHHH	31.52	21082442
275	Phosphorylation	SRLCGPESPPPPYSR HHHCCCCCCCCCCCC	45.93	30635358
436	Phosphorylation	RKVPPGYSIKVFDFE EECCCCCEEEEEEEC	23.11	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
436	S	Phosphorylation	Kinase	PRKX	Q922R0	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Wwp1	Q8BZZ3	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
174	K	ubiquitylation		15221015
Monoubiquitinated at Lys-174 by the E2/E3 hybrid ubiquitin-protein ligase UBE2O, leading to reduced binding affinity for the activated BMP type I receptor ACVR1/ALK2, thereby enhancing BMP7 and regulating adipocyte differentiation (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SMAD6_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AXIN1_MOUSE	Axin1	physical	16601693
BMR1A_MOUSE	Bmpr1a	physical	20663871
BMPR2_MOUSE	Bmpr2	physical	20663871
ACVR1_MOUSE	Acvr1	physical	20663871
TBX6_MOUSE	Tbx6	physical	19561075
SMUF1_MOUSE	Smurf1	physical	19561075

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SMAD6_MOUSE

Related Literatures of Post-Translational Modification