SHPRH_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SHPRH_HUMAN
• UniProt AC: Q149N8
• Protein Name: E3 ubiquitin-protein ligase SHPRH
• Gene Name: SHPRH
• Organism: Homo sapiens (Human).
• Sequence Length: 1683
• Protein Description: E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'..
• Protein Sequence: MSSRRKRAPPVRVDEEKRQQLHWNMHEDRRNEPIIISDDDEQPCPGSDTSSAHYIILSDSLKEEVAHRDKKRCSKVVSFSKPIEKEETVGIFSPLSVKLNIVISPYHFDNSWKAFLGELTLQLLPAQSLIENFSERSITLMSSESSNQFLIYVHSKGEDVEKQKKEPMSICDKGILVESSFSGEMLEDLGWLQKKRRIKLYQKPEGNHIIKVGIYLLEAGLAKLDFLSDANSRMKKFNQLMKKVMEKLHNSIIPDVLEEDEDDPESEPEGQDIDELYHFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQECFRSSPATESALHFLWREIVTSEGLKLYYNPYTGCIIREYPNSGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYFGGKLKKTEIQNIEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDICDKTKKQAVGSPRKIQKETRKSGNKDTDSEYLPSDTSDDDDDPYYYYYKSRRNRSKLRKKLVPSTKKGKSQPFINPDSQGHCPATSDSGITDVAMSKSTCISEFNQEHETEDCAESLNHADSDVPPSNTMSPFNTSDYRFECICGELDQIDRKPRVQCLKCHLWQHAKCVNYDEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDVVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLIARHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALYPVQQTIHELQRKIHSNSPWWLNVIHRAIEFTIDEELVQRVRNEITSNYKQQTGKLSMSEKFRDCRGLQFLLTTQMEELNKCQKLVREAVKNLEGPPSRNVIESATVCHLRPARLPLNCCVFCKADELFTEYESKLFSNTVKGQTAIFEEMIEDEEGLVDDRAPTTTRGLWAISETERSMKAILSFAKSHRFDVEFVDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKRDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAERSHTNSSAKHSEASVLTVADLADLFTKETEELE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
37 (in isoform 2)	Phosphorylation	-	25.28	29978859
37	Phosphorylation	RNEPIIISDDDEQPC CCCCEEEECCCCCCC	25.28	23663014
47	Phosphorylation	DEQPCPGSDTSSAHY CCCCCCCCCCCCCEE	23.90	30576142
49	Phosphorylation	QPCPGSDTSSAHYII CCCCCCCCCCCEEEE	26.69	27251275
50	Phosphorylation	PCPGSDTSSAHYIIL CCCCCCCCCCEEEEE	30.44	27251275
51	Phosphorylation	CPGSDTSSAHYIILS CCCCCCCCCEEEEEC	22.58	27251275
54	Phosphorylation	SDTSSAHYIILSDSL CCCCCCEEEEECHHH	6.86	27251275
58	Phosphorylation	SAHYIILSDSLKEEV CCEEEEECHHHHHHH	18.10	27251275
60	Phosphorylation	HYIILSDSLKEEVAH EEEEECHHHHHHHHH	37.66	27251275
78	Phosphorylation	KRCSKVVSFSKPIEK HHCCEEEEECCCCCC	27.13	23186163
81	Acetylation	SKVVSFSKPIEKEET CEEEEECCCCCCCCC	48.26	22424773
81	Ubiquitination	SKVVSFSKPIEKEET CEEEEECCCCCCCCC	48.26	-
88	Phosphorylation	KPIEKEETVGIFSPL CCCCCCCCEEECCCC	25.73	25850435
93	Phosphorylation	EETVGIFSPLSVKLN CCCEEECCCCEEEEE	24.02	21815630
93 (in isoform 4)	Phosphorylation	-	24.02	-
96	Phosphorylation	VGIFSPLSVKLNIVI EEECCCCEEEEEEEE	22.16	25850435
215	Phosphorylation	HIIKVGIYLLEAGLA CEEEEEEEEHHHHHH	10.17	29978859
228	Phosphorylation	LAKLDFLSDANSRMK HHHHHHCCHHHHHHH	34.37	29978859
232	Phosphorylation	DFLSDANSRMKKFNQ HHCCHHHHHHHHHHH	35.16	29978859
251	Phosphorylation	VMEKLHNSIIPDVLE HHHHHHHCCCHHHHC	15.90	20873877
266 (in isoform 4)	Phosphorylation	-	64.93	-
266	Phosphorylation	EDEDDPESEPEGQDI CCCCCCCCCCCCCCH	64.93	25159151
277	Phosphorylation	GQDIDELYHFVKQTH CCCHHHHHHHHHHHH	7.37	20873877
351	Phosphorylation	LKLYYNPYTGCIIRE CEEEECCCCCEEEEE	16.48	24719451
352	Phosphorylation	KLYYNPYTGCIIREY EEEECCCCCEEEEEC	27.17	24719451
379	Acetylation	ADEMGLGKTVEVLAL HHCCCCCHHHHHHHH	55.21	7925301
420	Acetylation	PSHYFGGKLKKTEIQ CHHHCCCCCCCCEEC	58.47	25953088
422	Ubiquitination	HYFGGKLKKTEIQNI HHCCCCCCCCEECCC	62.22	-
423	Sumoylation	YFGGKLKKTEIQNIE HCCCCCCCCEECCCC	62.92	-
423	Ubiquitination	YFGGKLKKTEIQNIE HCCCCCCCCEECCCC	62.92	-
423	Sumoylation	YFGGKLKKTEIQNIE HCCCCCCCCEECCCC	62.92	-
434	Ubiquitination	QNIEFEPKEKVQCPP CCCCCCCCHHCCCCC	64.07	-
481	Phosphorylation	YDVQRNRSLLKRMLK HHHHHCHHHHHHHHH	41.42	24719451
512	Ubiquitination	SGTFTLGKNYKEEDI CCEEECCCCCCHHHC	61.28	-
530	Phosphorylation	TKKQAVGSPRKIQKE HHHHHCCCCHHHHHH	18.22	30266825
546	Phosphorylation	RKSGNKDTDSEYLPS HHCCCCCCCCCCCCC	42.93	30576142
555	Phosphorylation	SEYLPSDTSDDDDDP CCCCCCCCCCCCCCC	38.09	30576142
567	Phosphorylation	DDPYYYYYKSRRNRS CCCHHHHHHCCCCHH	6.08	26657352
569	Phosphorylation	PYYYYYKSRRNRSKL CHHHHHHCCCCHHHH	21.98	26657352
597	Phosphorylation	QPFINPDSQGHCPAT CCCCCCCCCCCCCCC	39.04	26714015
597 (in isoform 4)	Phosphorylation	-	39.04	-
635	Phosphorylation	ETEDCAESLNHADSD CCHHHHHHCCCCCCC	18.88	-
691	Phosphorylation	QHAKCVNYDEKNLKI HCCCCCCCCCCCCCC	12.70	-
787	Phosphorylation	NYVDIPHSNSEDGRR CCCCCCCCCCCCHHH	36.38	-
789	Phosphorylation	VDIPHSNSEDGRRLR CCCCCCCCCCHHHHH	39.83	-
801	Phosphorylation	RLRNQKRYMAIPSPL HHHCCCCCCCCCCCH	9.64	18083107
812 (in isoform 5)	Ubiquitination	-	31.42	21890473
830	Ubiquitination	MVECPTVKAAEMAQR HCCCCHHHHHHHHHH	44.34	-
839	Phosphorylation	AEMAQRLSGINRWCI HHHHHHHCCCCCCCC	39.55	-
894	Phosphorylation	KNPQHLYSFIAKILW CCHHHHHHHHHHHHH	19.28	24719451
934	Phosphorylation	SPVERHFYHRQHEVC CHHHHCCHHCCCCCC	7.03	18083107
951	"N6,N6-dimethyllysine"	DVVVKLRKISDWALK HHHHHHHHHHHHHHH	57.93	-
951	Methylation	DVVVKLRKISDWALK HHHHHHHHHHHHHHH	57.93	-
972	Phosphorylation	RTVTSILYPLLRLRQ HHHHHHHHHHHHHHH	7.08	-
997	Phosphorylation	EFLPLQKSTMTMEEL CCCCCCCCCCCHHHH	15.51	24043423
998	Phosphorylation	FLPLQKSTMTMEELL CCCCCCCCCCHHHHH	24.56	24043423
1000	Phosphorylation	PLQKSTMTMEELLTS CCCCCCCCHHHHHHH	22.86	24043423
1006	Phosphorylation	MTMEELLTSLQKKCG CCHHHHHHHHHHHHC	39.76	24043423
1007	Phosphorylation	TMEELLTSLQKKCGT CHHHHHHHHHHHHCC	28.86	24043423
1010	Ubiquitination	ELLTSLQKKCGTECE HHHHHHHHHHCCHHH	56.15	-
1011	Ubiquitination	LLTSLQKKCGTECEE HHHHHHHHHCCHHHH	25.57	-
1062	Ubiquitination	EEHKGKLKTDSLQRL HHHCCCCCHHHHHHH	54.49	-
1106	Phosphorylation	AKQLREHYMSKCNTE HHHHHHHHHHHHHHH	9.79	28348404
1108	Phosphorylation	QLREHYMSKCNTEVA HHHHHHHHHHHHHHH	27.22	28348404
1176	Acetylation	NYKQQTGKLSMSEKF CHHHHHCCCCHHHHH	40.55	25953088
1180	Phosphorylation	QTGKLSMSEKFRDCR HHCCCCHHHHHHHCC	33.97	23025827
1256	Ubiquitination	LFTEYESKLFSNTVK HHHHHHHHHHCCCCC	41.43	21890473
1256 (in isoform 1)	Ubiquitination	-	41.43	21890473
1260 (in isoform 4)	Ubiquitination	-	41.37	21890473
1300	Phosphorylation	AISETERSMKAILSF EEHHHHHHHHHHHHH	20.57	23403867
1306	Phosphorylation	RSMKAILSFAKSHRF HHHHHHHHHHHHCCC	19.62	20068231
1310 (in isoform 4)	Phosphorylation	-	19.23	-
1310	Phosphorylation	AILSFAKSHRFDVEF HHHHHHHHCCCEEEE	19.23	20068231
1350	Phosphorylation	MALRNRVSAVDELAM HHHHHCHHHHHHHHH	21.24	22210691
1359	Phosphorylation	VDELAMATERLRVRD HHHHHHHHHHHCCCC	14.46	22210691
1391	Ubiquitination	EVEQNRIKLLNDKAV HHHHHHHHHHCCHHH	43.62	-
1396	Ubiquitination	RIKLLNDKAVATSQL HHHHHCCHHHHHHHH	43.62	-
1400	Phosphorylation	LNDKAVATSQLQKKL HCCHHHHHHHHHHHH	15.48	26270265
1401	Phosphorylation	NDKAVATSQLQKKLG CCHHHHHHHHHHHHH	21.11	26270265
1405	Ubiquitination	VATSQLQKKLGQLLY HHHHHHHHHHHHHHH	59.57	-
1422	Ubiquitination	NLEKSQDKTSGGVNP CCCCCCCCCCCCCCC	36.63	-
1468	Phosphorylation	IEQYSVGSHRSSIKC HHHHCCCCCCCCCEE	17.36	25690035
1483	Phosphorylation	AICRQTTSHKEISYV EEEECCCCCCEEEEE	35.63	30576142
1488	Phosphorylation	TTSHKEISYVFTSEK CCCCCEEEEEEECCC	19.14	-
1492	Phosphorylation	KEISYVFTSEKANQE CEEEEEEECCCCCCC	26.40	30576142
1493	Phosphorylation	EISYVFTSEKANQEE EEEEEEECCCCCCCC	26.15	30576142
1495	Acetylation	SYVFTSEKANQEEDI EEEEECCCCCCCCCC	53.02	20167786
1562	Ubiquitination	FAQISRVKTFQENLS HHHHHHHHHHHHHHH	42.38	21890473
1562 (in isoform 1)	Ubiquitination	-	42.38	21890473
1566 (in isoform 4)	Ubiquitination	-	56.82	21890473
1572	Ubiquitination	QENLSAFKRDPQINI HHHHHHHCCCCCCEE	56.46	2189047
1572 (in isoform 1)	Ubiquitination	-	56.46	21890473
1576 (in isoform 4)	Ubiquitination	-	45.72	21890473
1647	Ubiquitination	ERMQAMLKTAERSHT HHHHHHHHHHHHHCC	32.38	-
1676	Phosphorylation	ADLADLFTKETEELE HHHHHHHHHCHHHCC	34.96	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SHPRH_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SHPRH_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SHPRH_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FDFT_HUMAN	FDFT1	physical	16169070
FBXW7_HUMAN	FBXW7	physical	16169070
RAD18_HUMAN	RAD18	physical	21396873
UB2V2_HUMAN	UBE2V2	physical	17108083
UBE2N_HUMAN	UBE2N	physical	17108083
UBE2A_HUMAN	UBE2A	physical	17108083
RAD18_HUMAN	RAD18	physical	17108083
PCNA_HUMAN	PCNA	physical	17108083
RAD18_HUMAN	RAD18	physical	25023518
RPA1_HUMAN	POLR1A	physical	28400511
UBF1_HUMAN	UBTF	physical	28400511
H31_HUMAN	HIST1H3A	physical	28400511

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.