ROGF1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ROGF1_ARATH
• UniProt AC: Q93ZY2
• Protein Name: Rop guanine nucleotide exchange factor 1
• Gene Name: ROPGEF1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 548
• Subcellular Localization: Cytoplasm, cytosol. Cell membrane . Localizes to the apical region of the pollen tube plasma membrane to activate ARAC11/ROP1 and interacts with ARAC10/ROP11 on plasma membrane in guard cells.
• Protein Description: Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Acts downstream of PRK2 in the control of polarized pollen tube growth by activating ARAC11/ROP1. In association with ROPGEF4, acts as specific regulator of ARAC10/ROP11 function in ABA-mediated stomatal closure. May play a role in the Rac/Rop-signaling pathway that controls ROS-mediated root hair development..
• Protein Sequence: MGSLSSEEDDEVSSERCGSYSPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVAGRGFYFPAPVMLPVIGGKDVVWDDKQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLSLFSINGLNPQKAFSVQSSPHGSPFATPALSVASRSPRRAPPLYSVKRNGTREKGIVGETEKAWSYAGNLSSRRVTGVTPERD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
46	Phosphorylation	RFDGEGASSSIPSSP ECCCCCCCCCCCCCC	35.16	25561503
47	Phosphorylation	FDGEGASSSIPSSPR CCCCCCCCCCCCCCC	31.58	25561503
48	Phosphorylation	DGEGASSSIPSSPRV CCCCCCCCCCCCCCE	35.70	25561503
51	Phosphorylation	GASSSIPSSPRVVAG CCCCCCCCCCCEEEC	51.83	25561503
52	Phosphorylation	ASSSIPSSPRVVAGR CCCCCCCCCCEEECC	16.03	25561503
108	Phosphorylation	LLLGEDMSGGGKGVC HHHCCCCCCCCHHHH	46.75	24894044
235	Phosphorylation	CDKDSYNSPASVRQE CCCCCCCCCCCCCCC	17.38	30291188
460	Phosphorylation	DAMRRSISVTESLSL HHHHCCCCCCCCEEE	25.00	-
480	Phosphorylation	LNPQKAFSVQSSPHG CCHHCCEECCCCCCC	24.89	-
544	Phosphorylation	SRRVTGVTPERD--- CCCCCCCCCCCC---	22.30	25561503

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ROGF1_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
460	S	Phosphorylation	Phosphorylated at Ser-460 and Ser-480 by PRK2.	23024212
480	S	Phosphorylation	Phosphorylated at Ser-460 and Ser-480 by PRK2.	23024212

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ROGF1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAC5_ARATH	ARAC5	physical	15980860
RAC1_ARATH	ARAC1	physical	15980860
RAC2_ARATH	RAC2	physical	15980860
RAC8_ARATH	ROP10	physical	15980860
FERON_ARATH	FER	physical	20876100
RAC11_ARATH	ROP1	physical	16415208

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.