RNF4_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RNF4_RAT
• UniProt AC: O88846
• Protein Name: E3 ubiquitin-protein ligase RNF4
• Gene Name: Rnf4
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 194
• Subcellular Localization: Cytoplasm. Nucleus. Nucleus, PML body. Nucleus, nucleoplasm.
• Protein Description: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation..
• Protein Sequence: MSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Ubiquitination	STRNPQRKRRGGAVN CCCCHHHHCCCCCCC	41.00	-
94	Phosphorylation	LRQDHADSCVVSSDD CCHHCCCCEEECCCH	15.03	25575281
98	Phosphorylation	HADSCVVSSDDEELS CCCCEEECCCHHHHH	13.98	27097102
99	Phosphorylation	ADSCVVSSDDEELSK CCCEEECCCHHHHHC	37.15	27097102
105	Phosphorylation	SSDDEELSKDKDVYV CCCHHHHHCCCCEEE	41.93	25575281
111	Phosphorylation	LSKDKDVYVTTHTPR HHCCCCEEEEECCCC	11.38	25575281
113	Phosphorylation	KDKDVYVTTHTPRST CCCCEEEEECCCCCC	8.65	25575281
114	Phosphorylation	DKDVYVTTHTPRSTK CCCEEEEECCCCCCC	17.44	25575281
116	Phosphorylation	DVYVTTHTPRSTKDE CEEEEECCCCCCCCC	20.50	25575281

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Rnf4	O88846	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RNF4_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RNF4_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UB2D1_HUMAN	UBE2D1	physical	22842904
UBC_HUMAN	UBC	physical	22842904
RNF4_RAT	Rnf4	physical	22842904
H2A3_RAT	Hist3h2a	physical	11319220
H2B1_RAT	Hist1h2bl	physical	11319220
RNF4_RAT	Rnf4	physical	24882209
UB2D1_HUMAN	UBE2D1	physical	24882209

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.