dbPTM

H2B1_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	H2B1_RAT
UniProt AC	Q00715
Protein Name	Histone H2B type 1
Gene Name
Organism	Rattus norvegicus (Rat).
Sequence Length	125
Subcellular Localization	Nucleus. Chromosome.
Protein Description	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid (By similarity)..
Protein Sequence	MPEPAKSRPAPKKGSKKAVTKAQKKDGKERKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGERRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MPEPAKSRP ------CCCCCCCCC	57.17	-
6	Acetylation	--MPEPAKSRPAPKK --CCCCCCCCCCCCC	58.68	16283522
6	N6-crotonyl-L-lysine	--MPEPAKSRPAPKK --CCCCCCCCCCCCC	58.68	-
6	Butyrylation	--MPEPAKSRPAPKK --CCCCCCCCCCCCC	58.68	-
6	Lactoylation	--MPEPAKSRPAPKK --CCCCCCCCCCCCC	58.68	-
6	Other	--MPEPAKSRPAPKK --CCCCCCCCCCCCC	58.68	-
6	Crotonylation	--MPEPAKSRPAPKK --CCCCCCCCCCCCC	58.68	-
12	Crotonylation	AKSRPAPKKGSKKAV CCCCCCCCCCCHHHH	72.39	-
12	Other	AKSRPAPKKGSKKAV CCCCCCCCCCCHHHH	72.39	-
12	Lactoylation	AKSRPAPKKGSKKAV CCCCCCCCCCCHHHH	72.39	-
12	N6-crotonyl-L-lysine	AKSRPAPKKGSKKAV CCCCCCCCCCCHHHH	72.39	-
12	Acetylation	AKSRPAPKKGSKKAV CCCCCCCCCCCHHHH	72.39	25786129
13	Other	KSRPAPKKGSKKAVT CCCCCCCCCCHHHHH	68.32	-
13	Crotonylation	KSRPAPKKGSKKAVT CCCCCCCCCCHHHHH	68.32	-
13	N6-crotonyl-L-lysine	KSRPAPKKGSKKAVT CCCCCCCCCCHHHHH	68.32	-
13	Acetylation	KSRPAPKKGSKKAVT CCCCCCCCCCHHHHH	68.32	16283522
15	Phosphorylation	RPAPKKGSKKAVTKA CCCCCCCCHHHHHHH	39.60	-
16	N6-crotonyl-L-lysine	PAPKKGSKKAVTKAQ CCCCCCCHHHHHHHH	54.11	-
16	Acetylation	PAPKKGSKKAVTKAQ CCCCCCCHHHHHHHH	54.11	16283522
16	Crotonylation	PAPKKGSKKAVTKAQ CCCCCCCHHHHHHHH	54.11	-
16	Lactoylation	PAPKKGSKKAVTKAQ CCCCCCCHHHHHHHH	54.11	-
17	Glutarylation	APKKGSKKAVTKAQK CCCCCCHHHHHHHHH	50.19	-
17	Crotonylation	APKKGSKKAVTKAQK CCCCCCHHHHHHHHH	50.19	-
17	Acetylation	APKKGSKKAVTKAQK CCCCCCHHHHHHHHH	50.19	25786129
17	N6-crotonyl-L-lysine	APKKGSKKAVTKAQK CCCCCCHHHHHHHHH	50.19	-
17	Lactoylation	APKKGSKKAVTKAQK CCCCCCHHHHHHHHH	50.19	-
21	Lactoylation	GSKKAVTKAQKKDGK CCHHHHHHHHHHCCH	42.07	-
21	Butyrylation	GSKKAVTKAQKKDGK CCHHHHHHHHHHCCH	42.07	-
21	Other	GSKKAVTKAQKKDGK CCHHHHHHHHHHCCH	42.07	-
21	Acetylation	GSKKAVTKAQKKDGK CCHHHHHHHHHHCCH	42.07	16283522
21	N6-crotonyl-L-lysine	GSKKAVTKAQKKDGK CCHHHHHHHHHHCCH	42.07	-
21	Crotonylation	GSKKAVTKAQKKDGK CCHHHHHHHHHHCCH	42.07	-
24	Acetylation	KAVTKAQKKDGKERK HHHHHHHHHCCHHHH	58.66	25786129
24	Lactoylation	KAVTKAQKKDGKERK HHHHHHHHHCCHHHH	58.66	-
24	N6-crotonyl-L-lysine	KAVTKAQKKDGKERK HHHHHHHHHCCHHHH	58.66	-
24	Crotonylation	KAVTKAQKKDGKERK HHHHHHHHHCCHHHH	58.66	-
24	Other	KAVTKAQKKDGKERK HHHHHHHHHCCHHHH	58.66	-
25	Other	AVTKAQKKDGKERKR HHHHHHHHCCHHHHH	60.68	-
25	Acetylation	AVTKAQKKDGKERKR HHHHHHHHCCHHHHH	60.68	26302492
33	Phosphorylation	DGKERKRSRKESYSV CCHHHHHHHHHHHHH	51.22	23984901
35	Acetylation	KERKRSRKESYSVYV HHHHHHHHHHHHHHH	52.86	72631205
35	N6-crotonyl-L-lysine	KERKRSRKESYSVYV HHHHHHHHHHHHHHH	52.86	-
35	Glutarylation	KERKRSRKESYSVYV HHHHHHHHHHHHHHH	52.86	-
35	Crotonylation	KERKRSRKESYSVYV HHHHHHHHHHHHHHH	52.86	-
35	Succinylation	KERKRSRKESYSVYV HHHHHHHHHHHHHHH	52.86	26843850
35	Other	KERKRSRKESYSVYV HHHHHHHHHHHHHHH	52.86	-
37	Phosphorylation	RKRSRKESYSVYVYK HHHHHHHHHHHHHHH	26.24	27097102
38	Phosphorylation	KRSRKESYSVYVYKV HHHHHHHHHHHHHHH	11.84	27097102
39	Phosphorylation	RSRKESYSVYVYKVL HHHHHHHHHHHHHHH	19.09	27097102
41	Phosphorylation	RKESYSVYVYKVLKQ HHHHHHHHHHHHHHH	7.66	27097102
43	Phosphorylation	ESYSVYVYKVLKQVH HHHHHHHHHHHHHHC	4.25	-
44	Glutarylation	SYSVYVYKVLKQVHP HHHHHHHHHHHHHCC	30.92	-
44	Other	SYSVYVYKVLKQVHP HHHHHHHHHHHHHCC	30.92	-
44	Lactoylation	SYSVYVYKVLKQVHP HHHHHHHHHHHHHCC	30.92	-
44	Acetylation	SYSVYVYKVLKQVHP HHHHHHHHHHHHHCC	30.92	25786129
47	Other	VYVYKVLKQVHPDTG HHHHHHHHHHCCCCC	53.94	-
47	Glutarylation	VYVYKVLKQVHPDTG HHHHHHHHHHCCCCC	53.94	-
47	Acetylation	VYVYKVLKQVHPDTG HHHHHHHHHHCCCCC	53.94	25786129
47	Methylation	VYVYKVLKQVHPDTG HHHHHHHHHHCCCCC	53.94	-
47	Ubiquitination	VYVYKVLKQVHPDTG HHHHHHHHHHCCCCC	53.94	-
53	Phosphorylation	LKQVHPDTGISSKAM HHHHCCCCCCCHHHH	40.65	22673903
56	Phosphorylation	VHPDTGISSKAMGIM HCCCCCCCHHHHHHH	27.42	27097102
57	Phosphorylation	HPDTGISSKAMGIMN CCCCCCCHHHHHHHH	23.63	27097102
58	"N6,N6-dimethyllysine"	PDTGISSKAMGIMNS CCCCCCHHHHHHHHH	35.29	-
58	Methylation	PDTGISSKAMGIMNS CCCCCCHHHHHHHHH	35.29	-
58	Other	PDTGISSKAMGIMNS CCCCCCHHHHHHHHH	35.29	-
65	Phosphorylation	KAMGIMNSFVNDIFE HHHHHHHHHHHHHHH	17.12	22108457
79	Methylation	ERIAGERRLAHYNKR HHHHCHHHHHHCCCC	31.74	-
85	Other	RRLAHYNKRSTITSR HHHHHCCCCCCCCHH	40.33	-
85	Lactoylation	RRLAHYNKRSTITSR HHHHHCCCCCCCCHH	40.33	-
85	Methylation	RRLAHYNKRSTITSR HHHHHCCCCCCCCHH	40.33	-
85	Acetylation	RRLAHYNKRSTITSR HHHHHCCCCCCCCHH	40.33	25786129
85	"N6,N6,N6-trimethyllysine"	RRLAHYNKRSTITSR HHHHHCCCCCCCCHH	40.33	-
86	Methylation	RLAHYNKRSTITSRE HHHHCCCCCCCCHHH	35.08	-
92	Methylation	KRSTITSREIQTAVR CCCCCCHHHHHHHHH	36.07	-
108	Glutarylation	LLPGELAKHAVSEGT HCCHHHHHHHHHHHH	44.73	-
108	Lactoylation	LLPGELAKHAVSEGT HCCHHHHHHHHHHHH	44.73	-
108	Other	LLPGELAKHAVSEGT HCCHHHHHHHHHHHH	44.73	-
108	Ubiquitination	LLPGELAKHAVSEGT HCCHHHHHHHHHHHH	44.73	-
108	Methylation	LLPGELAKHAVSEGT HCCHHHHHHHHHHHH	44.73	-
108	Acetylation	LLPGELAKHAVSEGT HCCHHHHHHHHHHHH	44.73	25786129
112	Phosphorylation	ELAKHAVSEGTKAVT HHHHHHHHHHHHHHH	30.96	29779826
112	O-linked_Glycosylation	ELAKHAVSEGTKAVT HHHHHHHHHHHHHHH	30.96	-
115	Phosphorylation	KHAVSEGTKAVTKYT HHHHHHHHHHHHHHC	16.27	19346237
116	Other	HAVSEGTKAVTKYTS HHHHHHHHHHHHHCC	52.28	-
116	Acetylation	HAVSEGTKAVTKYTS HHHHHHHHHHHHHCC	52.28	22902405
116	Succinylation	HAVSEGTKAVTKYTS HHHHHHHHHHHHHCC	52.28	-
116	Glutarylation	HAVSEGTKAVTKYTS HHHHHHHHHHHHHCC	52.28	-
116	Lactoylation	HAVSEGTKAVTKYTS HHHHHHHHHHHHHCC	52.28	-
116	Ubiquitination	HAVSEGTKAVTKYTS HHHHHHHHHHHHHCC	52.28	-
116	Methylation	HAVSEGTKAVTKYTS HHHHHHHHHHHHHCC	52.28	-
119	Phosphorylation	SEGTKAVTKYTSSK- HHHHHHHHHHCCCC-	24.16	25575281
120	Other	EGTKAVTKYTSSK-- HHHHHHHHHCCCC--	39.67	-
120	Glutarylation	EGTKAVTKYTSSK-- HHHHHHHHHCCCC--	39.67	-
120	Lactoylation	EGTKAVTKYTSSK-- HHHHHHHHHCCCC--	39.67	-
120	Acetylation	EGTKAVTKYTSSK-- HHHHHHHHHCCCC--	39.67	25786129
120	Succinylation	EGTKAVTKYTSSK-- HHHHHHHHHCCCC--	39.67	-
120	Ubiquitination	EGTKAVTKYTSSK-- HHHHHHHHHCCCC--	39.67	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
15	S	Phosphorylation	Kinase	MST1	-	Uniprot
37	S	Phosphorylation	Kinase	AMPK	Q09137	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
4	K	Methylation	-
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci.
4	K	Methylation	-
Similarly, monoubiquitination at Lys-120 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.
4	K	ubiquitylation	-
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci.
4	K	ubiquitylation	-
Similarly, monoubiquitination at Lys-120 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.
15	S	Phosphorylation	-
Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation.
15	S	Phosphorylation	-
Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
35	K	Methylation	-
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci.
35	K	ubiquitylation	-
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci.
37	S	Phosphorylation	-
Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity).
79	K	Methylation	-
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci.
79	K	Methylation	-
Similarly, monoubiquitination at Lys-120 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.
79	K	ubiquitylation	-
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci.
79	K	ubiquitylation	-
Similarly, monoubiquitination at Lys-120 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.
112	S	ubiquitylation	-
GlcNAcylation at Ser-112 promotes monoubiquitination of Lys-120.
120	K	ubiquitylation	-
Similarly, monoubiquitination at Lys-120 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.
120	K	ubiquitylation	-
GlcNAcylation at Ser-112 promotes monoubiquitination of Lys-120.
120	K	Methylation	-
Similarly, monoubiquitination at Lys-120 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of H2B1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of H2B1_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of H2B1_RAT

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Inhibition of core histones acetylation by carcinogenic nickel(II)."; Golebiowski F., Kasprzak K.S.; Mol. Cell. Biochem. 279:133-139(2005). Cited for: ACETYLATION AT LYS-6; LYS-13; LYS-16 AND LYS-21.	16283522 [Abstract]

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