RHG32_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RHG32_MOUSE
• UniProt AC: Q811P8
• Protein Name: Rho GTPase-activating protein 32
• Gene Name: Arhgap32
• Organism: Mus musculus (Mouse).
• Sequence Length: 2089
• Subcellular Localization: Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell projection, dendritic spine . Cytoplasm, cell cortex . Endosome membrane . Golgi apparatus membrane . Endoplasmic reticulum membrane . Membrane . Association to membrane
• Protein Description: GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity..
• Protein Sequence: METESETSSLGDDSVFWLDCEGVTQLTDGDEEEREESFRKMKSSIHSEEDDFVPELHRNVHPRERPDWEETLSAMARGADVPEIPGDLTLKSCGSTASTKVKHVKKLPFTKGHFPKMAECAHFHYENVEFGSIQLSLSEEQNEVMKNGCESKELVYLVQIACQGKSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLTELPRSDVLKDSPESVTQMLTAYLSRLSTIAGNKINCGPALTWMEIDNKGNHLLVHEESSINTPAVGAAHVIKRYTARAPDELTLEVGDIVSVIDMPPKVLSTWWRGKHGFQVGLFPGHCVELINQKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILKERVFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRHEFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSGKISAVMQEGAASLSRPKSLLVSSPSTKLLTLEEAQARTQAQVSSPIVTENKYIEVGEGPAALQGKFHTVIEFPLERKRPQNKMKKSPVGSWRSFFNLGKSSSVSKRKLQRNESEPSEMKAMALKGGRAEGTLRSAKSEESLTSLHAVDGDSKLFRPRRPRSSSDALSASFNGDVLGNRCNSYDNLPHDNESEEEVGLLHIPALVSPHSAEDVDLSPPDIGVASLDFDPMSFQCSPPKAESECLESGASFLDSLGYTRDKLSPSKKDAEAGGSQSQTPGSTASSEPVSPVQEKLSPFFTLDLSPTDDKSSKPSSFTEKVVYAFSPKIGRKLSKSPSMNISEPISVTLPPRVSEVIGTVSNTVAQNASPTSWDKSVEERDVINRSPTQLQLGKMKAGEREAQETCEPEAQPLEQGAAEEVELPGTEERPVLSSQSKAVPSGQSQTGAVTHDPPQDPVPVSSVSLIPPPPPPKNVARMLALALAESAQQASSQTLKRPGASQAGCTSYGDTAVVPSEEKLPSSYSSLTLDKTCFQTDRPAEQFHPQINGLGNCNQPLPEAAAMGGPTQSNTTDSGEQLHQVDLIGNSLHRNHISGDPEKARSTSAPLTDSEKSDDHGSFPEDHAGKSSVSTVSFLEQDQSPLHFSCGDQPLSYLGTSVDKPHHSSELTDKSPMPSTLPRDKAHHPLSGSPEENSSTATMAYMMATPARAEPSNSEASRVLAEQPSAADFVAATLQRTHRTNRPLPPPPSQRPAEQPPVVGQVQEAPSIGLNNSHKVQGTAPAPERPPESRAMGDPAPIFLSDGTAAAQCPMGASAPQPGLPEKVRESSRAPPLHLRAESFPGHSCGFAAPVPPTRTMESKMAAALHSSAADATSSSNYHSFVPSSASVDDVMPVPLPVSQPKHASQKIAYSSFARPDVTAEPFGPENCLHFNMTPNCQFRPQSVPPHHNKLEPHQVYGARSEPPASMGPRYNTYVAPGRNMSGHHSKPCSRVEYVSSLGSSVRNPCCPEDILPYPTIRRVQSLHAPPPSMIRSVPISRTEVPPDDEPAYCPRPVYQYKPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYSSSSSSYYSPEGALCDVDAYGTVQLRPLHRLSSRDFAFYNPRLQGKNVYNYAGLPPRPRANATGYFSGNDHNVVTMPPTADGKHTYTSWDLEDMEKYRMQSIRRESRARQKVKGPIMSQYDNMTPAVQEDLGGIYVIHLRSKSDPGKTGLLSVAEGKEGRHPAKAVSPEGDERFYRKHPESEFDRAHHHGGYGSTQAEKPSLPQKQSSLRNRKLHDMGCSLPEHRAHQEASHRQLCESKNGPPYPQGAGQLDYGSKGMPDTSEPSNYHNSGKYMTSGQGSLTLNHKEVRLPKDLDRPRARQPPGPEKHSRDCYKEEEHFSQSMVPPPKPERSHSLKLHHTQNLERDPSVLYQYQTHSKRQSSMTVVSQYDNLEDYHSLPQHQRGGFGGAGMGAYVPSGFVHPQSRTYATALGQGAFLPTELSLPHPDTQIHAE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
43	Phosphorylation	ESFRKMKSSIHSEED HHHHHHHHHHHCCCC	30.90	26643407
44	Phosphorylation	SFRKMKSSIHSEEDD HHHHHHHHHHCCCCC	20.85	26643407
47	Phosphorylation	KMKSSIHSEEDDFVP HHHHHHHCCCCCCHH	40.80	26643407
270	Phosphorylation	AAHVIKRYTARAPDE HHHHHHHHCCCCCCC	10.35	-
271	Phosphorylation	AHVIKRYTARAPDEL HHHHHHHCCCCCCCE	17.44	-
279	Phosphorylation	ARAPDELTLEVGDIV CCCCCCEEEEECCEE	20.87	-
287	Phosphorylation	LEVGDIVSVIDMPPK EEECCEEEEEECCHH	17.43	-
381	Phosphorylation	LGEHLLNSGFEVPQV HHHHHHHCCCCHHHH	44.84	22807455
459	Phosphorylation	ELPNPLLTYQLYEKF CCCCCCHHHHHHHHH	19.53	29514104
463	Phosphorylation	PLLTYQLYEKFSDAV CCHHHHHHHHHHHHH	11.10	29514104
543	Phosphorylation	ESACFSGTAAFMEVR HHHHHCCCHHHHHHH	17.39	22807455
572	Phosphorylation	VLFSGKISAVMQEGA HHHCCHHHHHHHCCH	20.49	29899451
581	Phosphorylation	VMQEGAASLSRPKSL HHHCCHHHCCCCCCE	26.75	29899451
583	Phosphorylation	QEGAASLSRPKSLLV HCCHHHCCCCCCEEE	44.00	29899451
587	Phosphorylation	ASLSRPKSLLVSSPS HHCCCCCCEEECCCC	29.62	26824392
591	Phosphorylation	RPKSLLVSSPSTKLL CCCCEEECCCCCCEE	36.18	29472430
592	Phosphorylation	PKSLLVSSPSTKLLT CCCEEECCCCCCEEE	18.52	25521595
594	Phosphorylation	SLLVSSPSTKLLTLE CEEECCCCCCEEEHH	39.87	25521595
595	Phosphorylation	LLVSSPSTKLLTLEE EEECCCCCCEEEHHH	29.15	29472430
612	Phosphorylation	ARTQAQVSSPIVTEN HHHHHHCCCCEEECC	20.51	25619855
613	Phosphorylation	RTQAQVSSPIVTENK HHHHHCCCCEEECCC	21.57	25619855
621	Phosphorylation	PIVTENKYIEVGEGP CEEECCCEEEECCCC	17.56	29514104
655	Phosphorylation	PQNKMKKSPVGSWRS CCCCCCCCCCCCHHH	21.21	26643407
659	Phosphorylation	MKKSPVGSWRSFFNL CCCCCCCCHHHHHHC	21.39	26643407
662	Phosphorylation	SPVGSWRSFFNLGKS CCCCCHHHHHHCCCC	28.12	26643407
682	Phosphorylation	RKLQRNESEPSEMKA HHHCCCCCCCHHHHH	59.36	25521595
685	Phosphorylation	QRNESEPSEMKAMAL CCCCCCCHHHHHHHH	46.39	22324799
700	Phosphorylation	KGGRAEGTLRSAKSE HCCCCCCCCCCCCCH	15.54	25338131
703	Phosphorylation	RAEGTLRSAKSEESL CCCCCCCCCCCHHHC	43.06	24925903
706	Phosphorylation	GTLRSAKSEESLTSL CCCCCCCCHHHCHHE	46.44	25521595
709	Phosphorylation	RSAKSEESLTSLHAV CCCCCHHHCHHEEEC	32.66	24925903
711	Phosphorylation	AKSEESLTSLHAVDG CCCHHHCHHEEECCC	38.22	25619855
712	Phosphorylation	KSEESLTSLHAVDGD CCHHHCHHEEECCCC	24.37	24925903
720	Phosphorylation	LHAVDGDSKLFRPRR EEECCCCCCCCCCCC	36.47	25619855
730	Phosphorylation	FRPRRPRSSSDALSA CCCCCCCCCCCCCHH	36.56	23527152
731	Phosphorylation	RPRRPRSSSDALSAS CCCCCCCCCCCCHHH	33.25	26643407
732	Phosphorylation	PRRPRSSSDALSASF CCCCCCCCCCCHHHC	28.31	21082442
736	Phosphorylation	RSSSDALSASFNGDV CCCCCCCHHHCCCCC	24.48	26643407
738	Phosphorylation	SSDALSASFNGDVLG CCCCCHHHCCCCCCC	18.83	19060867
821	Phosphorylation	SGASFLDSLGYTRDK HCHHHHHHCCCCCCC	26.33	26643407
824	Phosphorylation	SFLDSLGYTRDKLSP HHHHHCCCCCCCCCC	12.14	26643407
825	Phosphorylation	FLDSLGYTRDKLSPS HHHHCCCCCCCCCCC	30.50	26643407
830	Phosphorylation	GYTRDKLSPSKKDAE CCCCCCCCCCHHHHH	32.63	24719451
832	Phosphorylation	TRDKLSPSKKDAEAG CCCCCCCCHHHHHCC	49.30	26643407
841	Phosphorylation	KDAEAGGSQSQTPGS HHHHCCCCCCCCCCC	26.24	25619855
843	Phosphorylation	AEAGGSQSQTPGSTA HHCCCCCCCCCCCCC	37.41	25619855
845	Phosphorylation	AGGSQSQTPGSTASS CCCCCCCCCCCCCCC	34.56	23984901
848	Phosphorylation	SQSQTPGSTASSEPV CCCCCCCCCCCCCCC	23.23	25619855
849	Phosphorylation	QSQTPGSTASSEPVS CCCCCCCCCCCCCCC	36.15	25619855
851	Phosphorylation	QTPGSTASSEPVSPV CCCCCCCCCCCCCHH	35.01	25619855
852	Phosphorylation	TPGSTASSEPVSPVQ CCCCCCCCCCCCHHH	43.38	23375375
856	Phosphorylation	TASSEPVSPVQEKLS CCCCCCCCHHHHHCC	30.03	25521595
863	Phosphorylation	SPVQEKLSPFFTLDL CHHHHHCCCCEEEEC	30.63	25619855
867	Phosphorylation	EKLSPFFTLDLSPTD HHCCCCEEEECCCCC	21.53	25619855
871	Phosphorylation	PFFTLDLSPTDDKSS CCEEEECCCCCCCCC	25.97	25521595
873	Phosphorylation	FTLDLSPTDDKSSKP EEEECCCCCCCCCCC	54.15	25619855
877	Phosphorylation	LSPTDDKSSKPSSFT CCCCCCCCCCCCCCC	51.06	20415495
878	Phosphorylation	SPTDDKSSKPSSFTE CCCCCCCCCCCCCCC	55.09	20415495
881	Phosphorylation	DDKSSKPSSFTEKVV CCCCCCCCCCCCHHH	41.65	20415495
882	Phosphorylation	DKSSKPSSFTEKVVY CCCCCCCCCCCHHHE	44.72	20415495
889	Phosphorylation	SFTEKVVYAFSPKIG CCCCHHHEEECHHHC	12.62	25777480
892	Phosphorylation	EKVVYAFSPKIGRKL CHHHEEECHHHCCCC	19.67	25521595
902	Phosphorylation	IGRKLSKSPSMNISE HCCCCCCCCCCCCCC	20.87	25293948
904	Phosphorylation	RKLSKSPSMNISEPI CCCCCCCCCCCCCCE	31.39	26643407
908	Phosphorylation	KSPSMNISEPISVTL CCCCCCCCCCEEEEC	32.11	25293948
912	Phosphorylation	MNISEPISVTLPPRV CCCCCCEEEECCCCH	21.55	25293948
920	O-linked_Glycosylation	VTLPPRVSEVIGTVS EECCCCHHHHHCCCC	27.68	55413563
935	Phosphorylation	NTVAQNASPTSWDKS CHHHHCCCCCCCCCC	35.94	26643407
937	Phosphorylation	VAQNASPTSWDKSVE HHHCCCCCCCCCCHH	39.70	26643407
938	Phosphorylation	AQNASPTSWDKSVEE HHCCCCCCCCCCHHH	36.34	26643407
942	Phosphorylation	SPTSWDKSVEERDVI CCCCCCCCHHHHHHH	32.08	24925903
952	Phosphorylation	ERDVINRSPTQLQLG HHHHHCCCCCHHHHC	28.01	25521595
954	Phosphorylation	DVINRSPTQLQLGKM HHHCCCCCHHHHCCC	43.06	25521595
1027	O-linked_Glycosylation	PQDPVPVSSVSLIPP CCCCCCCCEEEECCC	20.96	22645316
1088	Phosphorylation	PSEEKLPSSYSSLTL CCCCCCCCCCCCCEE	52.96	29472430
1089	Phosphorylation	SEEKLPSSYSSLTLD CCCCCCCCCCCCEEC	27.39	29472430
1090	Phosphorylation	EEKLPSSYSSLTLDK CCCCCCCCCCCEECC	13.45	29472430
1091	Phosphorylation	EKLPSSYSSLTLDKT CCCCCCCCCCEECCH	22.33	29472430
1092	Phosphorylation	KLPSSYSSLTLDKTC CCCCCCCCCEECCHH	19.69	25521595
1094	Phosphorylation	PSSYSSLTLDKTCFQ CCCCCCCEECCHHHC	34.55	29472430
1169	Phosphorylation	DPEKARSTSAPLTDS CHHHHHCCCCCCCCC	24.29	29899451
1170	Phosphorylation	PEKARSTSAPLTDSE HHHHHCCCCCCCCCC	29.18	29899451
1174	Phosphorylation	RSTSAPLTDSEKSDD HCCCCCCCCCCCCCC	36.10	-
1176	Phosphorylation	TSAPLTDSEKSDDHG CCCCCCCCCCCCCCC	41.51	29899451
1179	Phosphorylation	PLTDSEKSDDHGSFP CCCCCCCCCCCCCCC	44.19	29899451
1184	Phosphorylation	EKSDDHGSFPEDHAG CCCCCCCCCCCCCCC	33.92	29899451
1206	Phosphorylation	SFLEQDQSPLHFSCG HHHCCCCCCCCCCCC	37.41	-
1242	Phosphorylation	DKSPMPSTLPRDKAH CCCCCCCCCCCCCCC	34.61	28059163
1253	Phosphorylation	DKAHHPLSGSPEENS CCCCCCCCCCCCCCC	41.49	29899451
1255	Phosphorylation	AHHPLSGSPEENSST CCCCCCCCCCCCCCH	26.26	29899451
1260	Phosphorylation	SGSPEENSSTATMAY CCCCCCCCCHHHHHH	32.14	-
1267	Phosphorylation	SSTATMAYMMATPAR CCHHHHHHHHHCCCC	4.07	29899451
1299	Phosphorylation	AADFVAATLQRTHRT HHHHHHHHHHHHHCC	17.43	26824392
1339	Phosphorylation	PSIGLNNSHKVQGTA CCCCCCCCCCCCCCC	24.50	22807455
1355	O-linked_Glycosylation	APERPPESRAMGDPA CCCCCCHHHCCCCCC	30.98	51504111
1355	Phosphorylation	APERPPESRAMGDPA CCCCCCHHHCCCCCC	30.98	-
1405	Phosphorylation	PLHLRAESFPGHSCG CCEEECCCCCCCCCC	35.44	26239621
1410	Phosphorylation	AESFPGHSCGFAAPV CCCCCCCCCCCCCCC	23.11	26239621
1422	O-linked_Glycosylation	APVPPTRTMESKMAA CCCCCCCCHHHHHHH	27.98	55413571
1453	Phosphorylation	SFVPSSASVDDVMPV HCCCCCCCCCCCCCC	28.20	22807455
1526	Asymmetric dimethylarginine	PHQVYGARSEPPASM CCCCCCCCCCCCHHC	36.87	-
1526	Methylation	PHQVYGARSEPPASM CCCCCCCCCCCCHHC	36.87	24129315
1536	Asymmetric dimethylarginine	PPASMGPRYNTYVAP CCHHCCCCCCCEECC	31.44	-
1536	Methylation	PPASMGPRYNTYVAP CCHHCCCCCCCEECC	31.44	24129315
1540	Phosphorylation	MGPRYNTYVAPGRNM CCCCCCCEECCCCCC	7.32	29514104
1560	Phosphorylation	KPCSRVEYVSSLGSS CCCCCCHHHHHCCCC	11.63	29514104
1563	Phosphorylation	SRVEYVSSLGSSVRN CCCHHHHHCCCCCCC	27.06	23970565
1588	Phosphorylation	PTIRRVQSLHAPPPS HHHHHHHCCCCCCHH	21.18	22324799
1599	Phosphorylation	PPPSMIRSVPISRTE CCHHHHCCCCCCCCC	22.71	-
1635	Phosphorylation	SSQARSDYHVTQLQP CCCCCCCCEEEEEEH	10.23	29514104
1662	Phosphorylation	PYSSSSSSYYSPEGA CCCCCCCCCCCCCCC	30.00	-
1676	Phosphorylation	ALCDVDAYGTVQLRP CCCCCCCCCEEEEEE	14.63	-
1688	Phosphorylation	LRPLHRLSSRDFAFY EEECCCCCCCCEEEE	24.23	26643407
1689	Phosphorylation	RPLHRLSSRDFAFYN EECCCCCCCCEEEEC	40.22	26643407
1698	Methylation	DFAFYNPRLQGKNVY CEEEECCCCCCCCCC	35.69	-
1799	Phosphorylation	VIHLRSKSDPGKTGL EEEEEECCCCCCCCC	50.73	29514104
1823	Phosphorylation	RHPAKAVSPEGDERF CCCCCCCCCCCCHHH	23.30	25521595
1876	Phosphorylation	KLHDMGCSLPEHRAH HHHHCCCCCHHHHHH	41.03	25521595
1923	Phosphorylation	DTSEPSNYHNSGKYM CCCCCCCCCCCCCCC	13.50	29514104
1929	Phosphorylation	NYHNSGKYMTSGQGS CCCCCCCCCCCCCCC	14.78	29899451
1936	Phosphorylation	YMTSGQGSLTLNHKE CCCCCCCCEEECCCE	15.42	29899451
2004	Phosphorylation	QNLERDPSVLYQYQT CCCCCCHHHHHEEEC	29.10	29899451
2007	Phosphorylation	ERDPSVLYQYQTHSK CCCHHHHHEEECCCC	11.74	29514104
2017	Phosphorylation	QTHSKRQSSMTVVSQ ECCCCCCCCEEEEEE	26.55	29899451
2018	Phosphorylation	THSKRQSSMTVVSQY CCCCCCCCEEEEEEC	15.33	22499769
2020	Phosphorylation	SKRQSSMTVVSQYDN CCCCCCEEEEEECCC	21.50	22499769
2023	O-linked_Glycosylation	QSSMTVVSQYDNLED CCCEEEEEECCCHHH	20.77	30455755
2023	Phosphorylation	QSSMTVVSQYDNLED CCCEEEEEECCCHHH	20.77	22499769
2025	Phosphorylation	SMTVVSQYDNLEDYH CEEEEEECCCHHHHH	10.17	22499769
2031	Phosphorylation	QYDNLEDYHSLPQHQ ECCCHHHHHCCCHHH	5.50	22499769
2033	Phosphorylation	DNLEDYHSLPQHQRG CCHHHHHCCCHHHCC	35.20	22499769
2039	Methylation	HSLPQHQRGGFGGAG HCCCHHHCCCCCCCC	45.11	24129315
2050	Phosphorylation	GGAGMGAYVPSGFVH CCCCCCCCCCCCCCC	13.73	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RHG32_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RHG32_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RHG32_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CTNB1_MOUSE	Ctnnb1	physical	12531901
DLG4_MOUSE	Dlg4	physical	12531901
CADH2_MOUSE	Cdh2	physical	12531901
NMDE2_MOUSE	Grin2b	physical	12531901
CDC42_MOUSE	Cdc42	physical	12531901
RAC1_MOUSE	Rac1	physical	12531901

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-706 AND SER-952, ANDMASS SPECTROMETRY.
PubMed: 19367708

"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations.";
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
Mol. Cell. Proteomics 6:283-293(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-856; SER-892 ANDSER-952, AND MASS SPECTROMETRY.
PubMed: 17114649