PSMD4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PSMD4_MOUSE
• UniProt AC: O35226
• Protein Name: 26S proteasome non-ATPase regulatory subunit 4
• Gene Name: Psmd4
• Organism: Mus musculus (Mouse).
• Sequence Length: 376
• Protein Description: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains..
• Protein Sequence: MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGTEDSDDALLKMTINQQEFGRPGLPDLSSMTEEEQIAYAMQMSLQGTEFSQESADMDASSAMDTSDPVKEEDDYDVMQDPEFLQSVLENLPGVDPNNAAIRSVMGALASQATKDGKNDKKEEEKK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
37	S-nitrosocysteine	QDAVNIVCHSKTRSN HHHHHEEEECCCCCC	2.38	-
37	Glutathionylation	QDAVNIVCHSKTRSN HHHHHEEEECCCCCC	2.38	24333276
37	S-nitrosylation	QDAVNIVCHSKTRSN HHHHHEEEECCCCCC	2.38	20925432
40	Ubiquitination	VNIVCHSKTRSNPEN HHEEEECCCCCCCCC	25.05	22790023
40 (in isoform 2)	Ubiquitination	-	25.05	22790023
41	Phosphorylation	NIVCHSKTRSNPENN HEEEECCCCCCCCCC	42.20	25293948
43	Phosphorylation	VCHSKTRSNPENNVG EEECCCCCCCCCCEE	63.07	25293948
53	Phosphorylation	ENNVGLITLANDCEV CCCEEEEEECCCCEE	25.51	25293948
74 (in isoform 2)	Ubiquitination	-	40.62	22790023
74	Ubiquitination	DTGRILSKLHTVQPK CCCHHHHHCCCCCCC	40.62	22790023
87	S-nitrosylation	PKGKITFCTGIRVAH CCCCEEEECHHHHHH	2.26	20925432
87	Glutathionylation	PKGKITFCTGIRVAH CCCCEEEECHHHHHH	2.26	24333276
87	S-nitrosocysteine	PKGKITFCTGIRVAH CCCCEEEECHHHHHH	2.26	-
122 (in isoform 2)	Ubiquitination	-	63.72	22790023
122	Ubiquitination	SPVEDNEKDLVKLAK CCCCCCHHHHHHHHH	63.72	-
122	Acetylation	SPVEDNEKDLVKLAK CCCCCCHHHHHHHHH	63.72	23236377
122	Malonylation	SPVEDNEKDLVKLAK CCCCCCHHHHHHHHH	63.72	26320211
126 (in isoform 2)	Ubiquitination	-	50.45	22790023
126	Ubiquitination	DNEKDLVKLAKRLKK CCHHHHHHHHHHHHH	50.45	-
126	Malonylation	DNEKDLVKLAKRLKK CCHHHHHHHHHHHHH	50.45	26320211
135 (in isoform 2)	Ubiquitination	-	48.25	22790023
135	Ubiquitination	AKRLKKEKVNVDIIN HHHHHHHCCCEEEEE	48.25	22790023
242 (in isoform 3)	Phosphorylation	-	24.24	25777480
242 (in isoform 2)	Phosphorylation	-	24.24	25777480
242 (in isoform 5)	Phosphorylation	-	24.24	25266776
242	Phosphorylation	ARRAAAASAAEAGIA HHHHHHHHHHHHCCC	24.24	25619855
250 (in isoform 2)	Phosphorylation	-	21.96	26824392
250 (in isoform 3)	Phosphorylation	-	21.96	26824392
250 (in isoform 5)	Phosphorylation	-	21.96	21149613
250	Phosphorylation	AAEAGIATPGTEDSD HHHHCCCCCCCCCCC	21.96	25521595
253 (in isoform 2)	Phosphorylation	-	37.51	26824392
253 (in isoform 3)	Phosphorylation	-	37.51	26824392
253 (in isoform 5)	Phosphorylation	-	37.51	21149613
253	Phosphorylation	AGIATPGTEDSDDAL HCCCCCCCCCCCHHH	37.51	22942356
256	Phosphorylation	ATPGTEDSDDALLKM CCCCCCCCCHHHHHH	31.03	25521595
259 (in isoform 2)	Phosphorylation	-	21.56	26824392
259 (in isoform 3)	Phosphorylation	-	21.56	26824392
262	Ubiquitination	DSDDALLKMTINQQE CCCHHHHHHHCCHHH	34.54	-
348 (in isoform 4)	Phosphorylation	-	35.82	25168779
353	Phosphorylation	PNNAAIRSVMGALAS CCHHHHHHHHHHHHH	15.44	29472430
360	Phosphorylation	SVMGALASQATKDGK HHHHHHHHHHHCCCC	22.62	29514104
364	Ubiquitination	ALASQATKDGKNDKK HHHHHHHCCCCCCCH	68.45	22790023
364	Acetylation	ALASQATKDGKNDKK HHHHHHHCCCCCCCH	68.45	23806337
364 (in isoform 2)	Ubiquitination	-	68.45	22790023
367 (in isoform 2)	Ubiquitination	-	72.59	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PSMD4_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PSMD4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PSMD4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NFE2_MOUSE	Nfe2	physical	10921894
UBC_MOUSE	Ubc	physical	10921894
PRS8_MOUSE	Psmc5	physical	21883213
PSB5_MOUSE	Psmb5	physical	21883213

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-250; THR-253 ANDSER-256, AND MASS SPECTROMETRY.
PubMed: 17242355

"Phosphoproteomic analysis of the developing mouse brain.";
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
Mol. Cell. Proteomics 3:1093-1101(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-250, AND MASSSPECTROMETRY.
PubMed: 15345747