PROF1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PROF1_MOUSE
• UniProt AC: P62962
• Protein Name: Profilin-1
• Gene Name: Pfn1
• Organism: Mus musculus (Mouse).
• Sequence Length: 140
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR (By similarity)..
• Protein Sequence: MAGWNAYIDSLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVSITPAEVGVLVGKDRSSFFVNGLTLGGQKCSVIRDSLLQDGEFTMDLRTKSTGGAPTFNVTVTMTAKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAGWNAYID ------CCCHHHHHH	31.54	-
7	Phosphorylation	-MAGWNAYIDSLMAD -CCCHHHHHHHHHCC	11.31	30387612
28	Phosphorylation	AIVGYKDSPSVWAAV EEEEECCCCCEEEEC	18.21	25521595
30	Phosphorylation	VGYKDSPSVWAAVPG EEECCCCCEEEECCC	33.95	25521595
38	Acetylation	VWAAVPGKTFVSITP EEEECCCCEEEEECH	32.49	22826441
38	Succinylation	VWAAVPGKTFVSITP EEEECCCCEEEEECH	32.49	23954790
39	Phosphorylation	WAAVPGKTFVSITPA EEECCCCEEEEECHH	34.87	23984901
42	Phosphorylation	VPGKTFVSITPAEVG CCCCEEEEECHHHEE	19.29	26643407
54	Malonylation	EVGVLVGKDRSSFFV HEEEEEECCCCCEEE	42.35	26073543
54	Ubiquitination	EVGVLVGKDRSSFFV HEEEEEECCCCCEEE	42.35	-
57	Phosphorylation	VLVGKDRSSFFVNGL EEEECCCCCEEECCE	41.45	19854140
58	Phosphorylation	LVGKDRSSFFVNGLT EEECCCCCEEECCEE	24.49	23984901
65	Phosphorylation	SFFVNGLTLGGQKCS CEEECCEEECCEEEE	25.42	22006019
70	Ubiquitination	GLTLGGQKCSVIRDS CEEECCEEEEEEEHH	31.58	22790023
71	Glutathionylation	LTLGGQKCSVIRDSL EEECCEEEEEEEHHH	2.78	24333276
77	Phosphorylation	KCSVIRDSLLQDGEF EEEEEEHHHCCCCEE	22.56	24719451
90	Phosphorylation	EFTMDLRTKSTGGAP EEEEEEEECCCCCCC	36.32	-
92	Phosphorylation	TMDLRTKSTGGAPTF EEEEEECCCCCCCEE	31.50	26643407
93	Phosphorylation	MDLRTKSTGGAPTFN EEEEECCCCCCCEEE	40.99	26643407
102	Phosphorylation	GAPTFNVTVTMTAKT CCCEEEEEEEECCCE	16.03	27357545
108	Acetylation	VTVTMTAKTLVLLMG EEEEECCCEEEEHHC	32.97	-
109	Phosphorylation	TVTMTAKTLVLLMGK EEEECCCEEEEHHCC	21.45	23882026
116	Ubiquitination	TLVLLMGKEGVHGGL EEEEHHCCCCCCCCC	37.01	22790023
126	Ubiquitination	VHGGLINKKCYEMAS CCCCCCCHHHHHHHH	36.91	22790023
126	Acetylation	VHGGLINKKCYEMAS CCCCCCCHHHHHHHH	36.91	23201123
127	Ubiquitination	HGGLINKKCYEMASH CCCCCCHHHHHHHHH	37.25	22790023
128	Glutathionylation	GGLINKKCYEMASHL CCCCCHHHHHHHHHH	3.57	24333276
128	S-nitrosylation	GGLINKKCYEMASHL CCCCCHHHHHHHHHH	3.57	22178444
128	S-nitrosocysteine	GGLINKKCYEMASHL CCCCCHHHHHHHHHH	3.57	-
129	Phosphorylation	GLINKKCYEMASHLR CCCCHHHHHHHHHHH	20.02	22499769
133	Phosphorylation	KKCYEMASHLRRSQY HHHHHHHHHHHHHCC	23.03	22499769
138	Phosphorylation	MASHLRRSQY----- HHHHHHHHCC-----	27.65	22479341

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
57	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
90	T	Phosphorylation	Kinase	PRKACA	P00517	GPS
92	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
93	T	Phosphorylation	Kinase	PRKACA	P00517	GPS
138	S	Phosphorylation	Kinase	ROCK1	P70335	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
138	S	Phosphorylation	Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PROF1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CLH1_MOUSE	Cltc	physical	9463375
TERA_MOUSE	Vcp	physical	9463375
HS71B_MOUSE	Hspa1b	physical	9463375
TBB5_MOUSE	Tubb5	physical	9463375
ACTB_MOUSE	Actb	physical	9463375
DYN1_MOUSE	Dnm1	physical	16319076
MYPOP_MOUSE	Mypop	physical	15615774
PCLO_MOUSE	Pclo	physical	10508862

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.