NTRK2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NTRK2_MOUSE
• UniProt AC: P15209
• Protein Name: BDNF/NT-3 growth factors receptor
• Gene Name: Ntrk2 {ECO:0000312|MGI:MGI:97384}
• Organism: Mus musculus (Mouse).
• Sequence Length: 821
• Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Endosome membrane ; Single-pass type I membrane protein . Early endosome membrane . Internalized to endosomes upon ligand-binding.
• Protein Description: Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia..
• Protein Sequence: MSPWLKWHGPAMARLWGLCLLVLGFWRASLACPTSCKCSSARIWCTEPSPGIVAFPRLEPNSVDPENITEILIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAYKAFLKNSNLRHINFTRNKLTSLSRRHFRHLDLSDLILTGNPFTCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNMPLANLQIPNCGLPSARLAAPNLTVEEGKSVTLSCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKSNEIPSTDVADQSNREHLSVYAVVVIASVVGFCLLVMLLLLKLARHSKFGMKGPASVISNDDDSASPLHHISNGSNTPSSSEGGPDAVIIGMTKIPVIENPQYFGITNSQLKPDTFVQHIKRHNIVLKRELGEGAFGKVFLAECYNLCPEQDKILVAVKTLKDASDNARKDFHREAELLTNLQHEHIVKFYGVCVEGDPLIMVFEYMKHGDLNKFLRAHGPDAVLMAEGNPPTELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGENLLVKIGDFGMSRDVYSTDYYRVGGHTMLPIRWMPPESIMYRKFTTESDVWSLGVVLWEIFTYGKQPWYQLSNNEVIECITQGRVLQRPRTCPQEVYELMLGCWQREPHTRKNIKSIHTLLQNLAKASPVYLDILG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	S-nitrosylation	FWRASLACPTSCKCS HHHHHHCCCCCCCCC	4.60	24895380
36	S-nitrosylation	SLACPTSCKCSSARI HHCCCCCCCCCCCEE	5.92	24895380
38	S-nitrosylation	ACPTSCKCSSARIWC CCCCCCCCCCCEEEE	4.57	24895380
45	S-nitrosylation	CSSARIWCTEPSPGI CCCCEEEECCCCCCE	2.62	24895380
67	N-linked_Glycosylation	PNSVDPENITEILIA CCCCCCCCHHHHEEE	52.44	-
95	N-linked_Glycosylation	EAYVGLRNLTIVDSG HHHEECCCCEEECCC	46.72	-
121	N-linked_Glycosylation	NSNLRHINFTRNKLT CCCCCCCEECHHHHH	26.55	-
178	N-linked_Glycosylation	TQDLYCLNESSKNMP CHHEEEECCCCCCCC	43.47	-
205	N-linked_Glycosylation	SARLAAPNLTVEEGK HHHEECCCEEEECCC	43.55	-
241	N-linked_Glycosylation	NLVSKHMNETSHTQG HHHHHHCCCCCCCCC	49.46	-
254	N-linked_Glycosylation	QGSLRITNISSDDSG CCCEEEEECCCCCCC	29.76	-
280	N-linked_Glycosylation	GEDQDSVNLTVHFAP CCCCCCCEEEEEECC	33.67	-
325	N-linked_Glycosylation	FYNGAILNESKYICT HHCCCCCCCCCEEEE	45.44	-
338	N-linked_Glycosylation	CTKIHVTNHTEYHGC EEEEEECCCCCCCEE	38.71	-
350	N-linked_Glycosylation	HGCLQLDNPTHMNNG CEEEECCCCCCCCCC	52.54	-
411	N-linked_Glycosylation	TDIGDTTNKSNEIPS CCCCCCCCCCCCCCC	48.10	-
464 (in isoform 2)	Ubiquitination	-	63.22	-
468	Phosphorylation	FGMKGPASVISNDDD CCCCCCCEEECCCCC	24.10	22807455
471 (in isoform 2)	Ubiquitination	-	31.86	-
471	Phosphorylation	KGPASVISNDDDSAS CCCCEEECCCCCCCC	31.86	29899451
476	Phosphorylation	VISNDDDSASPLHHI EECCCCCCCCCCCCC	37.33	22817900
478	Phosphorylation	SNDDDSASPLHHISN CCCCCCCCCCCCCCC	31.76	17341134
484	Phosphorylation	ASPLHHISNGSNTPS CCCCCCCCCCCCCCC	30.68	19060867
487	Phosphorylation	LHHISNGSNTPSSSE CCCCCCCCCCCCCCC	41.61	19060867
489	Phosphorylation	HISNGSNTPSSSEGG CCCCCCCCCCCCCCC	26.67	20047950
491	Phosphorylation	SNGSNTPSSSEGGPD CCCCCCCCCCCCCCC	44.45	21082442
492	Phosphorylation	NGSNTPSSSEGGPDA CCCCCCCCCCCCCCC	33.34	22807455
515	Phosphorylation	PVIENPQYFGITNSQ CEECCCCCCCCCCCC	12.67	21562256
524	Ubiquitination	GITNSQLKPDTFVQH CCCCCCCCCCCHHHH	32.96	22790023
571	Ubiquitination	DKILVAVKTLKDASD CEEEEEEEEHHHCCH	38.10	22790023
701	Phosphorylation	FGMSRDVYSTDYYRV CCCCCCCEECCCEEE	15.33	9582017
702	Phosphorylation	GMSRDVYSTDYYRVG CCCCCCEECCCEEEC	18.04	22817900
703	Phosphorylation	MSRDVYSTDYYRVGG CCCCCEECCCEEECC	15.44	28066266
705	Phosphorylation	RDVYSTDYYRVGGHT CCCEECCCEEECCEE	8.02	9582017
706	Phosphorylation	DVYSTDYYRVGGHTM CCEECCCEEECCEEE	11.13	9582017
712	Phosphorylation	YYRVGGHTMLPIRWM CEEECCEEEEEEECC	24.55	22807455
726	Phosphorylation	MPPESIMYRKFTTES CCCHHHCCEEECCHH	14.69	-
782	Phosphorylation	RTCPQEVYELMLGCW CCCCHHHHHHHHHHH	11.55	8226808
800	Ubiquitination	PHTRKNIKSIHTLLQ CCHHHCHHHHHHHHH	53.39	22790023
801	Phosphorylation	HTRKNIKSIHTLLQN CHHHCHHHHHHHHHH	18.79	29899451
804	Phosphorylation	KNIKSIHTLLQNLAK HCHHHHHHHHHHHHH	27.74	29899451
816	Phosphorylation	LAKASPVYLDILG-- HHHHCCEEHHCCC--	11.31	10533983

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
478	S	Phosphorylation	Kinase	CDK5	P49615	PSP
515	Y	Phosphorylation	Kinase	NTRK2	P15209	PhosphoELM
701	Y	Phosphorylation	Kinase	NTRK2	P15209	PhosphoELM
705	Y	Phosphorylation	Kinase	NTRK2	P15209	PhosphoELM
706	Y	Phosphorylation	Kinase	NTRK2	P15209	PhosphoELM
816	Y	Phosphorylation	Kinase	NTRK2	P15209	PhosphoELM

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NTRK2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NTRK2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SQSTM_MOUSE	Sqstm1	physical	21574998
PLCG1_MOUSE	Plcg1	physical	23424281
GAB1_MOUSE	Gab1	physical	23424281
DLG4_MOUSE	Dlg4	physical	23424281
UCHL1_MOUSE	Uchl1	physical	28500221

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.