dbPTM

NOS2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NOS2_MOUSE
UniProt AC	P29477
Protein Name	Nitric oxide synthase, inducible
Gene Name	Nos2
Organism	Mus musculus (Mouse).
Sequence Length	1144
Subcellular Localization
Protein Description	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. [PubMed: 7503239 In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2]
Protein Sequence	MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLHVTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	S-palmitoylation	-----MACPWKFLFK -----CCCCHHHHEE	6.68	15485846
13	Phosphorylation	KFLFKVKSYQSDLKE HHHEEHHHHHHCCHH	31.24	30387612
14	Phosphorylation	FLFKVKSYQSDLKEE HHEEHHHHHHCCHHH	13.30	30635358
16	Phosphorylation	FKVKSYQSDLKEEKD EEHHHHHHCCHHHHC	36.36	30635358
31	Phosphorylation	INNNVKKTPCAVLSP CCCCCCCCCEEEECC	20.20	30635358
37	Phosphorylation	KTPCAVLSPTIQDDP CCCEEEECCCCCCCC	17.25	30635358
39	Phosphorylation	PCAVLSPTIQDDPKS CEEEECCCCCCCCCC	28.09	30635358
46	Phosphorylation	TIQDDPKSHQNGSPQ CCCCCCCCCCCCCCC	35.38	30635358
51	Phosphorylation	PKSHQNGSPQLLTGT CCCCCCCCCCCCCCC	19.61	30635358
56	Phosphorylation	NGSPQLLTGTAQNVP CCCCCCCCCCCCCCC	40.87	30635358
86	Phosphorylation	VRIKNWGSGEILHDT EEEECCCCCCCCHHH	25.52	26370283
422	Methylation	AVLHSFQKQNVTIMD EHHHHHHHCCCEEEE	41.66	-
569	Phosphorylation	KVVCMDQYKASTLEE EEEEECCHHCCCCCH	12.31	-
708	Phosphorylation	QQYRLIQSPEPLDLN HHEEECCCCCCCCHH	25.00	19144319
716	Methylation	PEPLDLNRALSSIHA CCCCCHHHHHHHHHH	44.17	5168487
733	Phosphorylation	VFTMRLKSQQNLQSE HHEEEHHHHHCCCCC	41.41	22817900
903	Phosphorylation	ILKPRYYSISSSQDH CCCCCEEECCCCCCC	13.68	22817900
1041	Methylation	QVHTGYSRLPGKPKV EECCCCCCCCCCCEE	36.10	5168485
1049	Phosphorylation	LPGKPKVYVQDILQK CCCCCEEEHHHHHHH	9.87	22817900
1134	Phosphorylation	SYGAKKGSALEEPKA CCCCCCCCCCCCCCC	38.26	30635358

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
733	S	Phosphorylation	Kinase	SGK1	Q9WVC6	PSP
903	S	Phosphorylation	Kinase	SGK1	Q9WVC6	PSP
1049	Y	Phosphorylation	Kinase	SRC	P12931	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NOS2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NOS2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PGH2_HUMAN	PTGS2	physical	16373578
ACTN4_MOUSE	Actn4	physical	12960352
NOS2_MOUSE	Nos2	physical	12437348

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NOS2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-708, AND MASSSPECTROMETRY.	19144319 [Abstract]