NGEF_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NGEF_HUMAN
• UniProt AC: Q8N5V2
• Protein Name: Ephexin-1
• Gene Name: NGEF
• Organism: Homo sapiens (Human).
• Sequence Length: 710
• Subcellular Localization: Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones (By similarity)..
• Protein Description: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity)..
• Protein Sequence: METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRMPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9 (in isoform 3)	Phosphorylation	-	6.80	28348404
9 (in isoform 2)	Phosphorylation	-	6.80	28348404
16	Phosphorylation	LEKTRRKSASDQWNT HHHHHHHCHHHHCCC	31.06	26657352
18	Phosphorylation	KTRRKSASDQWNTDN HHHHHCHHHHCCCCC	37.11	26657352
18 (in isoform 3)	Phosphorylation	-	37.11	28348404
18 (in isoform 2)	Phosphorylation	-	37.11	28348404
19 (in isoform 2)	Phosphorylation	-	58.17	24719451
19 (in isoform 3)	Phosphorylation	-	58.17	24719451
20 (in isoform 3)	Phosphorylation	-	32.59	28348404
20 (in isoform 2)	Phosphorylation	-	32.59	28348404
21 (in isoform 3)	Phosphorylation	-	18.06	28348404
21 (in isoform 2)	Phosphorylation	-	18.06	28348404
41 (in isoform 3)	Phosphorylation	-	27.51	-
41 (in isoform 2)	Phosphorylation	-	27.51	-
57 (in isoform 3)	Phosphorylation	-	4.61	24076635
57 (in isoform 2)	Phosphorylation	-	4.61	24076635
63	Phosphorylation	HIPIKRNSIFNRSIR CCCCCCCCCCCHHHH	32.37	23312004
149	Phosphorylation	EWPALADSPTTLTEA HCCHHCCCCCCHHHH	20.66	-
155 (in isoform 3)	Phosphorylation	-	49.14	24719451
179	Phosphorylation	IEQIGLLYQEYRDKS HHHHHHHHHHHCCHH	12.35	-
185	Ubiquitination	LYQEYRDKSTLQEIE HHHHHCCHHHHHHHH	36.02	-
186	Phosphorylation	YQEYRDKSTLQEIET HHHHCCHHHHHHHHH	38.34	29083192
187	Phosphorylation	QEYRDKSTLQEIETR HHHCCHHHHHHHHHH	37.90	29083192
193	Phosphorylation	STLQEIETRRQQDAE HHHHHHHHHHHHHHC	36.49	29083192
234	Ubiquitination	PASPPERKTLPQICL CCCCCCCCCCCHHHH	53.39	-
247	Phosphorylation	CLLSNPHSRFNLWQD HHCCCCCCCCCCCCC	39.56	24719451
283	Phosphorylation	EAMFELVTSEASYYK HHHHHHHHCCHHHHH	33.21	25850435
284	Phosphorylation	AMFELVTSEASYYKS HHHHHHHCCHHHHHH	24.56	25850435
287	Phosphorylation	ELVTSEASYYKSLNL HHHHCCHHHHHHHHH	25.58	25850435
288	Phosphorylation	LVTSEASYYKSLNLL HHHCCHHHHHHHHHH	23.20	25850435
289	Phosphorylation	VTSEASYYKSLNLLV HHCCHHHHHHHHHHH	7.51	25850435
291	Phosphorylation	SEASYYKSLNLLVSH CCHHHHHHHHHHHHH	13.33	24719451
297	Phosphorylation	KSLNLLVSHFMENER HHHHHHHHHHHCCHH	15.81	24719451
372 (in isoform 3)	Phosphorylation	-	9.90	24719451
464	Phosphorylation	GVRKMSRTEQMISIQ HHHHCCHHHHHHHHH	24.22	24719451
492	Ubiquitination	SHSRWLLKQGELQQM ECHHHHHHHCCHHHH	54.83	-
514 (in isoform 3)	Phosphorylation	-	7.16	24719451
537	Phosphorylation	RQIPGDKYQVFDSAP HCCCCCCCEEECCCC	18.04	-
578	Phosphorylation	NADDREATYMLKASS CCCHHHHHHHHHCCC	12.52	29083192
579	Phosphorylation	ADDREATYMLKASSQ CCHHHHHHHHHCCCH	13.90	29083192
584	Phosphorylation	ATYMLKASSQSEMKR HHHHHHCCCHHHHHH	27.59	29083192
585	Phosphorylation	TYMLKASSQSEMKRW HHHHHCCCHHHHHHH	42.45	29083192
587	Phosphorylation	MLKASSQSEMKRWMT HHHCCCHHHHHHHHH	41.42	29083192
594	Phosphorylation	SEMKRWMTSLAPNRR HHHHHHHHHHCCCCC	17.40	30622161
595	Phosphorylation	EMKRWMTSLAPNRRT HHHHHHHHHCCCCCH	13.36	30622161
602	Phosphorylation	SLAPNRRTKFVSFTS HHCCCCCHHEEEHHH	26.12	23312004
606	Phosphorylation	NRRTKFVSFTSRLLD CCCHHEEEHHHHHHC	26.41	30266825
608	Phosphorylation	RTKFVSFTSRLLDCP CHHEEEHHHHHHCCC	12.87	30266825
609	Phosphorylation	TKFVSFTSRLLDCPQ HHEEEHHHHHHCCCC	20.94	30266825
678	Phosphorylation	ILNPKIRSQNLKECF HHCHHHHCCCHHHHH	27.22	22798277
696	Phosphorylation	KMDDPQRSQNKDRRK CCCCHHHHCCHHHHH	32.62	22798277

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NGEF_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NGEF_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NGEF_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EPHA4_HUMAN	EPHA4	physical	11336673
KPCD_HUMAN	PRKCD	physical	28514442
KPCI_HUMAN	PRKCI	physical	28514442
MRS2_HUMAN	MRS2	physical	28514442
ACTY_HUMAN	ACTR1B	physical	28514442
ARP10_HUMAN	ACTR10	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.