NFE4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NFE4_HUMAN
• UniProt AC: Q86UQ8
• Protein Name: Transcription factor NF-E4
• Gene Name: NFE4
• Organism: Homo sapiens (Human).
• Sequence Length: 179
• Subcellular Localization: Nucleus .
• Protein Description: Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter..
• Protein Sequence: MPRVVCWHTLKSLNGYKNLSSGAETREGLRSSSPVDLPLRPRKQATAAGQRKLLSLQLLLCACTSVTDLTYWGPAGHGATAPHRSLLAIHLHLVPASSAAMKATGPHNAQTQVNPQGHAPSAEDPTGTWTVSGPCKDHPHPFLSQSNPPTRISSALPLKTDSALEQTPQQLPSLHLSQG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
43	Acetylation	DLPLRPRKQATAAGQ CCCCCCCCCCCHHHH	47.14	15273251
43	Ubiquitination	DLPLRPRKQATAAGQ CCCCCCCCCCCHHHH	47.14	1527325
126	Phosphorylation	APSAEDPTGTWTVSG CCCCCCCCCCEEEEC	60.61	-
132	Phosphorylation	PTGTWTVSGPCKDHP CCCCEEEECCCCCCC	29.64	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NFE4_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
43	K	Acetylation	Acetylation at Lys-43 prevents ubiquitination.	15273251
43	K	Acetylation	Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor at the gamma-promoter.	15273251
43	K	ubiquitylation	Acetylation at Lys-43 prevents ubiquitination.	15273251
43	K	ubiquitylation	Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor at the gamma-promoter.	15273251

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NFE4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ANM5_HUMAN	PRMT5	physical	19234465
KAT2B_HUMAN	KAT2B	physical	15273251
HDAC1_HUMAN	HDAC1	physical	15273251
TFCP2_HUMAN	TFCP2	physical	16263792
WDR5_HUMAN	WDR5	physical	22689669
ANM5_HUMAN	PRMT5	physical	22689669
NUCL_HUMAN	NCL	physical	22689669
ICLN_HUMAN	CLNS1A	physical	22689669

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Site-specific acetylation of the fetal globin activator NF-E4prevents its ubiquitination and regulates its interaction with thehistone deacetylase, HDAC1.";
Zhao Q., Cumming H., Cerruti L., Cunningham J.M., Jane S.M.;
J. Biol. Chem. 279:41477-41486(2004).
Cited for: INTERACTION WITH HDAC1 AND PCAF, UBIQUITINATION, ACETYLATION ATLYS-43, AND MUTAGENESIS OF LYS-43.
PubMed: 15273251