NEP2_SCHPO - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NEP2_SCHPO
• UniProt AC: O13612
• Protein Name: NEDD8-specific protease 2
• Gene Name: nep2
• Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
• Sequence Length: 415
• Subcellular Localization: Cytoplasm . Nucleus .
• Protein Description: Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as the pcu1, pcu2 and pcu4 cullins and other proteins (By similarity). Has a role in meiosis..
• Protein Sequence: MRSNSIFTKEIDSEAVKKSSNLRPPSTGSSNSNGSDTASPKKKKKGFFRSLFGSSSSGSKSCGSPFTRIWLEYFEVSLRKNDVDHFRPGYWILDTNIDFFYEIMLRQVLLKRPKEESQQIYLLRPAMVFFLAQAPNPLEIESALPPAMFDASFIFLPINDTNECGIESGSHWSLLVVSVEKGLGWYYDSMSNGNTNDCNLAIKNLGILLKKEFRVRHMKTPQQINDCDCGLHVCENTRILMYRLLQKPYVPKVDMNLDHSVVDSVRLRKALMEVITSLLAAYGSKVPKPSETHTDPEKDKKIFSKICKTEELLELPTLSAVTSDSAQPHSLPASMPSSQPQSRSESLPLTHPNSEPNPKLDSQPNSSPVRRPSLIKVKTASTSVLPTSILQRPPSIVPRPETAAIQHTQQSIEIH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MRSNSIFTKEID ---CCCCCCCCCCCC	21.96	24763107
8	Phosphorylation	MRSNSIFTKEIDSEA CCCCCCCCCCCCHHH	26.54	24763107
26	Phosphorylation	SSNLRPPSTGSSNSN HCCCCCCCCCCCCCC	48.35	25720772
27	Phosphorylation	SNLRPPSTGSSNSNG CCCCCCCCCCCCCCC	47.02	25720772
29	Phosphorylation	LRPPSTGSSNSNGSD CCCCCCCCCCCCCCC	26.83	25720772
30	Phosphorylation	RPPSTGSSNSNGSDT CCCCCCCCCCCCCCC	45.69	25720772
32	Phosphorylation	PSTGSSNSNGSDTAS CCCCCCCCCCCCCCC	44.51	25720772
35	Phosphorylation	GSSNSNGSDTASPKK CCCCCCCCCCCCCCC	35.72	28889911
37	Phosphorylation	SNSNGSDTASPKKKK CCCCCCCCCCCCCCC	30.63	25720772
56	Phosphorylation	RSLFGSSSSGSKSCG HHHHCCCCCCCCCCC	40.18	21712547
57	Phosphorylation	SLFGSSSSGSKSCGS HHHCCCCCCCCCCCC	49.38	21712547
362	Phosphorylation	EPNPKLDSQPNSSPV CCCCCCCCCCCCCCC	58.95	29996109
366	Phosphorylation	KLDSQPNSSPVRRPS CCCCCCCCCCCCCCH	42.58	24763107
367	Phosphorylation	LDSQPNSSPVRRPSL CCCCCCCCCCCCCHH	33.77	28889911
373	Phosphorylation	SSPVRRPSLIKVKTA CCCCCCCHHEEEEEC	40.70	24763107

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NEP2_SCHPO !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NEP2_SCHPO !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NEP2_SCHPO !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CUL1_SCHPO	cul1	physical	15769255
UBL1_SCHPO	ned8	physical	23496905
UBLH1_SCHPO	uch1	genetic	23496905
UBLH2_SCHPO	uch2	genetic	23496905
NESP1_SCHPO	nep1	genetic	23496905
CSN5_SCHPO	csn5	genetic	23496905

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of fission yeast.";
Wilson-Grady J.T., Villen J., Gygi S.P.;
J. Proteome Res. 7:1088-1097(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35 AND SER-367, AND MASSSPECTROMETRY.
PubMed: 18257517