NDF1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NDF1_MOUSE
• UniProt AC: Q60867
• Protein Name: Neurogenic differentiation factor 1
• Gene Name: Neurod1
• Organism: Mus musculus (Mouse).
• Sequence Length: 357
• Subcellular Localization: Cytoplasm. Nucleus . In pancreatic islet cells, shuttles to the nucleus in response to glucose stimulation. Colocalizes with NR0B2 in the nucleus (By similarity)..
• Protein Description: Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis..
• Protein Sequence: MTKSYSESGLMGEPQPQGPPSWTDECLSSQDEEHEADKKEDELEAMNAEEDSLRNGGEEEEEDEDLEEEEEEEEEEEDQKPKRRGPKKKKMTKARLERFKLRRMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKSPDLVSFVQTLCKGLSQPTTNLVAGCLQLNPRTFLPEQNPDMPPHLPTASASFPVHPYSYQSPGLPSPPYGTMDSSHVFHVKPPPHAYSAALEPFFESPLTDCTSPSFDGPLSPPLSINGNFSFKHEPSAEFEKNYAFTMHYPAATLAGPQSHGSIFSSGAAAPRCEIPIDNIMSFDSHSHHERVMSAQLNAIFHD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
162	Phosphorylation	EILRSGKSPDLVSFV HHHHCCCCHHHHHHH	27.46	22817900
259	Phosphorylation	ALEPFFESPLTDCTS CCHHHHCCCCCCCCC	21.76	15797719
266	Phosphorylation	SPLTDCTSPSFDGPL CCCCCCCCCCCCCCC	25.52	15797719
274	Phosphorylation	PSFDGPLSPPLSING CCCCCCCCCCEEECC	27.83	15797719
336	Phosphorylation	IPIDNIMSFDSHSHH CCCCCCCCCCCCCHH	23.31	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
162	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
162	S	Phosphorylation	Kinase	MK01	P63085	PhosphoELM
259	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
259	S	Phosphorylation	Kinase	MK01	P63085	PhosphoELM
266	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
266	S	Phosphorylation	Kinase	MK01	P63085	PhosphoELM
274	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
274	S	Phosphorylation	Kinase	MK01	P63085	PhosphoELM
336	S	Phosphorylation	Kinase	CAMK2	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
266	S	Phosphorylation	Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells.	12810726
274	S	Phosphorylation	In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization.	12810726
274	S	Phosphorylation	Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells.	12810726
336	S	Phosphorylation	In activated neurons, phosphorylated on Ser-336; which promotes dendritic growth (By similarity).	15797719

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NDF1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MIXL1_MOUSE	Mixl1	physical	20211142
NDF1_MOUSE	Neurod1	physical	15701640
RBL1_MOUSE	Rbl1	physical	15701640
NR4A1_MOUSE	Nr4a1	physical	15701640
RB_MOUSE	Rb1	physical	15701640

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Context-dependent regulation of NeuroD activity and proteinaccumulation.";
Dufton C., Marcora E., Chae J.H., McCullough J., Eby J., Hausburg M.,Stein G.H., Khoo S., Cobb M.H., Lee J.E.;
Mol. Cell. Neurosci. 28:727-736(2005).
Cited for: FUNCTION, PHOSPHORYLATION AT SER-259; SER-266 AND SER-274, ANDMUTAGENESIS OF SER-259; SER-266 AND SER-274.
PubMed: 15797719

"Regulation of insulin gene transcription by ERK1 and ERK2 inpancreatic beta cells.";
Khoo S., Griffen S.C., Xia Y., Baer R.J., German M.S., Cobb M.H.;
J. Biol. Chem. 278:32969-32977(2003).
Cited for: FUNCTION, HETERODIMERIZATION, PHOSPHORYLATION AT SER-162; SER-259;SER-266 AND SER-274, TISSUE SPECIFICITY, AND MUTAGENESIS OF SER-162;SER-259; SER-266 AND SER-274.
PubMed: 12810726